2. Apoptosis Metabolic Enzyme/Protease
  3. MDM-2/p53 HIF/HIF Prolyl-Hydroxylase
  4. Amifostine

Amifostine  (Synonyms: WR2721)

Cat. No.: HY-B0639 Purity: ≥98.0%
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Amifostine (WR2721) is a broad-spectrum cytoprotective agent and a radioprotector. Amifostine selectively protects normal tissues from damage caused by radiation and chemotherapy. Amifostine is potent hypoxia-inducible factor-α1 (HIF-α1) and p53 inducer. Amifostine protects cells from damage by scavenging oxygen-derived free radicals. Amifostine reduces renal toxicity and has antiangiogenic action.

For research use only. We do not sell to patients.

Amifostine Chemical Structure

Amifostine Chemical Structure

CAS No. : 20537-88-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 72 In-stock
Solution
10 mM * 1 mL in Water USD 72 In-stock
Solid
5 mg USD 41 In-stock
10 mg USD 65 In-stock
50 mg USD 272 In-stock
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Based on 7 publication(s) in Google Scholar

Other Forms of Amifostine:

Amifostine Related Antibodies

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Description

Amifostine (WR2721) is a broad-spectrum cytoprotective agent and a radioprotector. Amifostine selectively protects normal tissues from damage caused by radiation and chemotherapy. Amifostine is potent hypoxia-inducible factor-α1 (HIF-α1) and p53 inducer. Amifostine protects cells from damage by scavenging oxygen-derived free radicals. Amifostine reduces renal toxicity and has antiangiogenic action[1][2][3][4].

In Vitro

Amifostine (0.78125-100 μM, 24 h) reduces tert-Butyl hydroperoxide (TBHP)-induced cell damage in a dose-dependent manner and significantly reduces H9c2 cells apoptosis at a concentration of 100 μM[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Amifostine Related Antibodies
In Vivo

Amifostine (i.v., 400 mg/kg, 4 h) has a protective effect against myocardial I/R injury in male C57BL/6 mice[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice with myocardial I/R injury[5]
Dosage: 400 mg/kg
Administration: Intravenous injection; 4 hours
Result: Attenuated cardiomyocyte apoptosis and reduced the production of I/R-induced ROS.
Significantly reduced the expression of cleaved caspase 3 and Bax while enhanced the expression of SOD1, SOD2 and Bcl2.
Significantly increased SOD activity and reduced MDA levels.
Clinical Trial
Molecular Weight

214.22

Formula

C5H15N2O3PS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OP(SCCNCCCN)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (466.81 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6681 mL 23.3405 mL 46.6810 mL
5 mM 0.9336 mL 4.6681 mL 9.3362 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (466.81 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.6681 mL 23.3405 mL 46.6810 mL 116.7025 mL
5 mM 0.9336 mL 4.6681 mL 9.3362 mL 23.3405 mL
10 mM 0.4668 mL 2.3340 mL 4.6681 mL 11.6702 mL
15 mM 0.3112 mL 1.5560 mL 3.1121 mL 7.7802 mL
20 mM 0.2334 mL 1.1670 mL 2.3340 mL 5.8351 mL
25 mM 0.1867 mL 0.9336 mL 1.8672 mL 4.6681 mL
30 mM 0.1556 mL 0.7780 mL 1.5560 mL 3.8901 mL
40 mM 0.1167 mL 0.5835 mL 1.1670 mL 2.9176 mL
50 mM 0.0934 mL 0.4668 mL 0.9336 mL 2.3340 mL
60 mM 0.0778 mL 0.3890 mL 0.7780 mL 1.9450 mL
80 mM 0.0584 mL 0.2918 mL 0.5835 mL 1.4588 mL
100 mM 0.0467 mL 0.2334 mL 0.4668 mL 1.1670 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Amifostine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amifostine20537-88-6WR2721WR 2721WR-2721MDM-2/p53HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHBroad-spectrumradioprotectorcytoprotectivethiolantiangiogenicHIF-α1p53DNA-damageInhibitorinhibitorinhibit

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