2. GPCR/G Protein Neuronal Signaling Anti-infection
  3. Adrenergic Receptor Monoamine Oxidase Parasite
  4. Amitraz

Amitraz is a non-systemic acaricide and insecticide with alpha-adrenergic agonist activity that interacts with octopamine receptors in the central nervous system and inhibits monoamine oxidase and prostaglandin synthesis.

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Amitraz Chemical Structure

Amitraz Chemical Structure

CAS No. : 33089-61-1

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Other Forms of Amitraz:

Amitraz Related Antibodies

Top Publications Citing Use of Products

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α adrenergic receptor β adrenergic receptor

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MAO-A MAO-B

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Amitraz is a non-systemic acaricide and insecticide with alpha-adrenergic agonist activity that interacts with octopamine receptors in the central nervous system and inhibits monoamine oxidase and prostaglandin synthesis[1][2].

IC50 & Target

Mite

 

In Vitro

Amitraz (25-150 μM, 10 days) inhibits the activity of IRE/CTVM19 cells (from Ixodes ricinus) and modulates the expression of their ABC transporters[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Amitraz Related Antibodies

Real Time qPCR[1]

Cell Line: IRE/CTVM19 cells
Concentration: 25, 50, 100, 150 μM
Incubation Time: 10 days
Result: Resulted in a significant decrease in cell viability of IRE/CTVM19 cells compared to the control group (150 μM).

Real Time qPCR[1]

Cell Line: IRE/CTVM19 cells
Concentration: 25, 50, 100, 150 μM
Incubation Time: 10 days
Result: Induced a dose-dependent downregulation of ABCB1 gene expression and upregulates ABCB10 gene expression at 50 μM.
In Vivo

Amitraz (0-30 mg/kg, p.o., once daily for 19 weeks) exerts dose-dependent toxic effects on maternal and fetal development in pregnant female Sprague-Dawley rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Pregnant female sprague-Dawley rats (10 weeks old)[2]
Dosage: 0, 3, 10, 30 mg/kg
Administration: Oral gavage (p.o.), once daily for 19 days
Result: Exhibited significant maternal toxicity and developmental toxicity, including increased fetal mortality, reduced fetal weight, and abnormalities in external, visceral, and skeletal development (30 mg/kg/day).
Caused mild maternal toxicity and minimal developmental toxicity (10 mg/kg/day).
Did not induce maternal or developmental toxicity (3 mg/kg/day).
Molecular Weight

293.41

Formula

C19H23N3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

CN(/C=N/C1=CC=C(C)C=C1C)/C=N/C2=CC=C(C)C=C2C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (340.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ mg/mL

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4082 mL 17.0410 mL 34.0820 mL
5 mM 0.6816 mL 3.4082 mL 6.8164 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.52 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (8.52 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4082 mL 17.0410 mL 34.0820 mL 85.2050 mL
5 mM 0.6816 mL 3.4082 mL 6.8164 mL 17.0410 mL
10 mM 0.3408 mL 1.7041 mL 3.4082 mL 8.5205 mL
15 mM 0.2272 mL 1.1361 mL 2.2721 mL 5.6803 mL
20 mM 0.1704 mL 0.8521 mL 1.7041 mL 4.2603 mL
25 mM 0.1363 mL 0.6816 mL 1.3633 mL 3.4082 mL
30 mM 0.1136 mL 0.5680 mL 1.1361 mL 2.8402 mL
40 mM 0.0852 mL 0.4260 mL 0.8521 mL 2.1301 mL
50 mM 0.0682 mL 0.3408 mL 0.6816 mL 1.7041 mL
60 mM 0.0568 mL 0.2840 mL 0.5680 mL 1.4201 mL
80 mM 0.0426 mL 0.2130 mL 0.4260 mL 1.0651 mL
100 mM 0.0341 mL 0.1704 mL 0.3408 mL 0.8521 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Amitraz Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amitraz33089-61-1BTS-27419BTS27419BTS 27419Adrenergic ReceptorMonoamine OxidaseParasiteBeta ReceptorMAOIRE/CTVM19 cellsAnti-infectionMaternal toxicityDevelopmental toxicityInhibitorinhibitorinhibit

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