2. Autophagy
  3. Atg8/LC3 Autophagy
  4. Autophagy inducer 3

Autophagy inducer 3 has autophagy induced activity. Autophagy inducer 3 possesses robust autophagic cell death in diverse cancer cells sparing normal counterpart. Autophagy inducer 3 induces lethal autophagy by formation of characteristic autophagic vacuoles, LC3 puncta formation, upregulation of signature autophagy markers like Beclin and Atg family proteins.

For research use only. We do not sell to patients.

Autophagy inducer 3 Chemical Structure

Autophagy inducer 3 Chemical Structure

CAS No. : 2691054-63-2

Size Price Stock Quantity
5 mg USD 220 In-stock
10 mg USD 360 In-stock
25 mg USD 720 In-stock
50 mg USD 1150 In-stock
100 mg USD 1850 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Autophagy inducer 3 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Autophagy inducer 3 has autophagy induced activity. Autophagy inducer 3 possesses robust autophagic cell death in diverse cancer cells sparing normal counterpart. Autophagy inducer 3 induces lethal autophagy by formation of characteristic autophagic vacuoles, LC3 puncta formation, upregulation of signature autophagy markers like Beclin and Atg family proteins[1].

IC50 & Target

Autophagy[1]
In Vitro

Autophagy inducer 3 (compound 26b) (10 μM; 48 hours) effectively inhibits cell growth in diverse breast, lung and colon cancer cell lines[1].
Autophagy inducer 3 (0-10 μM; 48 hours) exhibits potent antiproliferative activity in COLO-205, LOVO, HT-29, DLD-1, SW48 and SW-620 with IC50s of 2.03 μM, 3.33 μM, 4.15 μM, 4.46 μM, 3.14 μM, 1.86 μM, respectively; and shows low cytotoxicity in normal human colon fibroblast CCD-18Co with IC50 over 10 μM[1].
Autophagy inducer 3 (7.5 μM; 18 hours) promotes non apoptotic cell death in DLD-1 cells by cellular granularity through vacuole formation and increase Annexin-V positive cells as well as PARP cleavage; and robustly induces the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Autophagy inducer 3 Related Antibodies

Cell Proliferation Assay

Cell Line: MCF-7, MDA-MB-231, DLD-1, HT-29, A549 and NCI-H358[1]
Concentration: 10 μM
Incubation Time: 48 hours
Result: Effectively inhibited cell growth at 10 μM dose in diverse cancer cell lines with the inhibition rates of 89.28~97.66%.

Cell Autophagy Assay

Cell Line: DLD-1[1]
Concentration: 7.5 μM
Incubation Time: 18 hours
Result: Promoted intracytoplasmic vacuole accumulation in colon cancer (DLD-1) cells; and robustly induced the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7.
Molecular Weight

377.60

Formula

C24H43NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCC[C@H](O)[C@@H](N)CC1=CC=C(C=C1)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 100 mg/mL (264.83 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6483 mL 13.2415 mL 26.4830 mL
5 mM 0.5297 mL 2.6483 mL 5.2966 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (6.62 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: 2.5 mg/mL (6.62 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.6483 mL 13.2415 mL 26.4831 mL 66.2076 mL
5 mM 0.5297 mL 2.6483 mL 5.2966 mL 13.2415 mL
10 mM 0.2648 mL 1.3242 mL 2.6483 mL 6.6208 mL
15 mM 0.1766 mL 0.8828 mL 1.7655 mL 4.4138 mL
20 mM 0.1324 mL 0.6621 mL 1.3242 mL 3.3104 mL
25 mM 0.1059 mL 0.5297 mL 1.0593 mL 2.6483 mL
30 mM 0.0883 mL 0.4414 mL 0.8828 mL 2.2069 mL
40 mM 0.0662 mL 0.3310 mL 0.6621 mL 1.6552 mL
50 mM 0.0530 mL 0.2648 mL 0.5297 mL 1.3242 mL
60 mM 0.0441 mL 0.2207 mL 0.4414 mL 1.1035 mL
80 mM 0.0331 mL 0.1655 mL 0.3310 mL 0.8276 mL
100 mM 0.0265 mL 0.1324 mL 0.2648 mL 0.6621 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Autophagy inducer 3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Autophagy inducer 32691054-63-2Autophagy inducer3Autophagy inducer-3Atg8/LC3AutophagyAutophagy-related 8| AnticancerNon apoptotic cell deathIntracytoplasmic vacuoleDLD-1Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Autophagy inducer 3
Cat. No.:
HY-146052
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.