Benzethonium chloride

Name: Benzethonium chloride
Brand: MedChemExpress
SKU: HY-B0942
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0942 Purity: 98.76%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Benzethonium chloride inhibits nicotinic acetylcholine receptors in human recombinant α7 and α4β2 neurons in Xenopus laevis oocytes, which has antibacterial, anticancer, antisepsis and disinfection activity. Benzethonium chloride induced Apoptosis and activated caspases in cancer cell lines. Benzethonium chloride ablates the tumor-forming ability of FaDu cells, delays the growth of xenograft tumors in vivo.

For research use only. We do not sell to patients.

Benzethonium chloride Chemical Structure

CAS No. : 121-54-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Benzethonium chloride:

Benzethonium-d₇ chloride Get quote
Benzethonium-d₆ chloride Get quote
Benzethonium chloride (Standard) Get quote

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	3.2 μM Compound: BztCl	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 23792315]
CCRF-CEM	IC₅₀	3.8 μM Compound: BztCl	Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTT assay Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTT assay	[PMID: 23792315]
HeLa	IC₅₀	5.7 μM Compound: BztCl	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 23792315]
HUVEC	IC₅₀	47 μM Compound: BztCl	Cytotoxicity against HUVEC after 72 hrs by MTT assay Cytotoxicity against HUVEC after 72 hrs by MTT assay	[PMID: 23792315]
MCF7	IC₅₀	5 μM Compound: BztCl	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 23792315]
MDA-MB-231	IC₅₀	4.8 μM Compound: BztCl	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 23792315]

In Vitro

Benzethonium chloride (0.1 g/L, 10 s) results in only a 50% reduction in bacterial number against S. mutans MT8148 and Streptococcus sobrinus^[1].
Benzethonium chloride (0.1-100 μM, 48 h) reduces cell viability against FaDu and C666-1 (both human cancer) cell lines with EC₅₀ values of 3.8 and 5.3 μM, respectively^[2].
Benzethonium chloride (9 μM, 24-48 h) induces apoptosis and caspase activation in FaDu cells^[2].
Benzethonium chloride (0.1 and 5 mg/L, 24-72 h) shows great acute toxicity to C. elegans, induces an inhibition on hatching and increases mortality in zebrafish embryos^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:	FaDu cells
Concentration:	9 μM
Incubation Time:	24-48 h
Result:	Revealed nuclear condensation and blebbing and Caspase-2, caspase-8, and caspase-9 activations, indicative of apoptosis, after 48 hours of treatment.

In Vivo

Benzethonium chloride (5 mg/kg, i.p., daily for 5 days) results in elimination of tumor formation and growth delay in established xenograft tumors^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Established xenograft tumors of mice^[2]
Dosage:	5 mg/kg
Administration:	i.p., daily for 5 days
Result:	Effectively eliminates the tumorforming potential of FaDu cells and survived for 3.5 days more than PBS-treated mice, which was a statistically significant delay.

Molecular Weight

448.08

Formula

C₂₇H₄₂ClNO₂

CAS No.

121-54-0

Appearance

Solid

Color

White to off-white

SMILES

CC(C)(C)CC(C1=CC=C(OCCOCC[N+](C)(C)CC2=CC=CC=C2)C=C1)(C)C.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (74.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (44.63 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2317 mL	11.1587 mL	22.3174 mL
5 mM	0.4463 mL	2.2317 mL	4.4635 mL
10 mM	0.2232 mL	1.1159 mL	2.2317 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 120 mg/mL (267.81 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.76%

Select Batch:

Data Sheet (272 KB) SDS (786 KB)

COA (244 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Maillard JY. Impact of benzalkonium chloride, benzethonium chloride and chloroxylenol on bacterial antimicrobial resistance. J Appl Microbiol. 2022 Dec;133(6):3322-3346. [Content Brief]

[2]. Yip KW, et al. Benzethonium chloride: a novel anticancer agent identified by using a cell-based small-molecule screen. Clin Cancer Res. 2006 Sep 15;12(18):5557-69. [Content Brief]

[3]. Sreevidya VS, et al. Benzalkonium chloride, benzethonium chloride, and chloroxylenol - Three replacement antimicrobials are more toxic than triclosan and triclocarban in two model organisms. Environ Pollut. 2018 Apr;235:814-824. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.2317 mL	11.1587 mL	22.3174 mL	55.7936 mL
	5 mM	0.4463 mL	2.2317 mL	4.4635 mL	11.1587 mL
	10 mM	0.2232 mL	1.1159 mL	2.2317 mL	5.5794 mL
	15 mM	0.1488 mL	0.7439 mL	1.4878 mL	3.7196 mL
	20 mM	0.1116 mL	0.5579 mL	1.1159 mL	2.7897 mL
	25 mM	0.0893 mL	0.4463 mL	0.8927 mL	2.2317 mL
	30 mM	0.0744 mL	0.3720 mL	0.7439 mL	1.8598 mL
	40 mM	0.0558 mL	0.2790 mL	0.5579 mL	1.3948 mL
DMSO	50 mM	0.0446 mL	0.2232 mL	0.4463 mL	1.1159 mL
DMSO	60 mM	0.0372 mL	0.1860 mL	0.3720 mL	0.9299 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.