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  4. Bleomycin sulfate

Bleomycin sulfate  (Synonyms: Bleomycin sulfate)

Cat. No.: HY-17565 Purity: 99.62%
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Bleomycin sulfate is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin sulfate is an antitumor antibiotic.

For research use only. We do not sell to patients.

Bleomycin sulfate Chemical Structure

Bleomycin sulfate Chemical Structure

CAS No. : 9041-93-4

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Based on 126 publication(s) in Google Scholar

Other Forms of Bleomycin sulfate:

Bleomycin sulfate Related Antibodies

Top Publications Citing Use of Products

115 Publications Citing Use of MCE Bleomycin sulfate

IHC
RT-PCR
IF
WB

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Apr 18;81(1):187.  [Abstract]

    Bleomycin sulfate (3 mg/kg; nebulized) significantly upregulates TRPA1 protein levels in mice.​

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Apr 18;81(1):187.  [Abstract]

    Bleomycin sulfate (3 mg/kg; nebulized) significantly increases TRPA1 mRNA levels in mouse lung tissues.​

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 3:131:155797.  [Abstract]

    PCF-EtOH blocked BLM (Bleomycin sulfate)-induced upregulation of β-catenin protein. All mice, except those in the control group (nebulization with saline), are nebulised with BLM/saline (1 mg/mL, 8 mL) for 30 min on days 1, 4 and 7, respectively.

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 3:131:155797.  [Abstract]

    PCF-EtOH diminished BLM (Bleomycin sulfate)-induced increase of fibronectin, vimentin and α-SMA. All mice, except those in the control group (nebulization with saline), are nebulised with BLM/saline (1 mg/mL, 8 mL) for 30 min on days 1, 4 and 7, respectively.

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Mech Ageing Dev. 2024 Jan 11:111902.  [Abstract]

    miR-129 delays bleomycin-induced cellular senescence. MRC5 cells were transfected with miR-Ctrl or miR-129 and then treated with bleomycin (1 µg/ml) where indicated. Four days after transfection, the p-H2AX, ITPR2, p16, p21 and GAPDH protein levels were quantified by western blot.

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Jun 16.  [Abstract]

    Bleomycin (1 mg/kg; single intratracheal injection). Representative results of coimmunostaining for Plekhf1 and F4/80 in lung sections.

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Jun 16.  [Abstract]

    Bleomycin (1 mg/kg; single intratracheal injection). Representative results of coimmunostaining for Plekhf1 and F4/80 in BALF cells from BLM-treated mice.

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Jun 16.  [Abstract]

    Bleomycin (1 mg/kg; single intratracheal injection). Results for time-course Western blot analysis of Plekhf1 and Arg1 (a macrophage M2 marker) and Col 1α (a fibrotic marker) expression in lung macrophages isolated from BLM-treated mice. Left panel: Representative Western blot images. Right panel: Bar graphs showing the data from three replicates.

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978.  [Abstract]

    Bleomycin (BLM) (5 mg/kg; intratracheal instillation) upregulates the mRNA levels of NLRP3, ASC, IL-1β, and IL-18 in BALB/c mice.

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978.  [Abstract]

    Bleomycin (BLM) (5 mg/kg; intratracheal instillation) significantly elevated the ratio of phosphorylated p65 to total p65 (p-p65/p65) and reduced the expression level of IκBα, indicating that NF-κB signaling pathway was activated in BLM-induced pulmonary fibrosis.

    Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978.  [Abstract]

    Bleomycin (BLM) (5 mg/kg; intratracheal instillation) increases the expression of NLRP3 in BALB/c mice.

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    Description

    Bleomycin sulfate is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin sulfate is an antitumor antibiotic[1].

    IC50 & Target

    DNA/RNA Synthesis[1]
    In Vitro

    Bleomycin (BLM) sulfate is chosen as the best-studied micronucleus (MN) inducers in human lymphocytes with different mechanisms of genotoxicity. The most frequent Bleomycin-induced DNA lesions are single and double strand breaks and single apuinic/apyrimidinic sites. At the same time Bleomycin is true radiomimetic compound, resembling almost completely the genetic effect of ionizing radiation[1].
    The IC50 value of Bleomycin sulfate for UT-SCC-19A cell line is 4.0±1.3 nM. UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin (BLM); IC50 values are 14.2±2.8 nM and 13.0±1.1 nM, respectively[2].
    Bleomycin sulfate (50, 100 μM; for 24, 48 h) induce pulmonary fibrosis in RLE-6TN cell (50 μM) and A549 cell (100 μM)[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Bleomycin sulfate Related Antibodies
    In Vivo

    Bleomycin sulfate can be used to induce pulmonary fibrosis models. After administration via intramuscular, subcutaneous, intraperitoneal, or intrapleural routes, Bleomycin sulfate is rapidly absorbed and reaches peak plasma concentrations in approximately 60 minutes. Less than 1% of intravenously injected Bleomycin sulfate binds to plasma proteins, ensuring high bioavailability. The mean plasma clearance rate of Bleomycin sulfate is about 70 mL/min/m2, indicating high plasma elimination and urinary excretion rates2[6].

    Induction of Pulmonary Fibrosis[7]
    Background
    Bleomycin sulfate can lead to lung patchy parenchymal inflammation, epithelial cell injury with reactive hyperplasia, epithelial-mesenchymal transition, activation and differentiation of fibroblasts to myofibroblasts, and basement membrane and alveolar epithelium injures. The experimental use of Bleomycin sulfate is to induce pulmonary fibrosis animal models.
    Specific Modeling Methods
    Mice: C57BL/6 • 12-week-old
    Administration: 3-5 mg/kg • intratracheal administration • sprays on day one
    Note
    The mice were housed in separate stainless-steel cages (six mice per cage) in a temperature-controlled environment (20-24°C) on 12 h light-dark cycles with unrestricted access to food and water.
    Modeling Indicators
    Body quality changes: The appetite activity is reduced, with the fur less shiny, the spirits being lethargic, and the bodyweight decreasing. Showed shortness of breath, coughing, and noisy.
    Lung changes: Increased fibrotic consolidations, non-aerated lung area, and high-density lung area. Pulmonary function decreased.
    Molecular changes: Increased indicators: TGF-β1, TNF-α, IL-6, and GM-CSF in bronchoalveolar lavage fluid.
    Correlated Product(s): Bleomycin hydrochloride (HY-17565A)
    Opposite Product(s): Neotuberostemonine (HY-N3196)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Fischer 344 rats, 8-10 week old, weighing 150-250 g[3]
    Dosage: 3.5-4 mg/kg
    Administration: Intra-tracheal
    Result: Body weights decreased by day 4 then increased by Day 7 through the end of the study.
    Clinical Trial
    Molecular Weight

    1523 (average)

    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C)N[C@H](C(N[C@H](C)[C@@H](O)[C@H](C)C(N[C@]([C@H](O)C)([H])C(NCCC2=NC(C3=NC(C(N[R])=O)=CS3)=CS2)=O)=O)=O)[C@H](C4=CN=CN4)O[C@H]5[C@H]([C@H]([C@@H]([C@@H](O5)CO)O)O)O[C@]6([H])[C@H]([C@H]([C@@H]([C@H](O6)CO)O)OC(N)=O)O.NC(NCCCC[R])=N.[R]CCC[S+](C)C.O=S(O)(O)=O.[R=].[Bleomycin A2].[R=].[Bleomycin B2]
    Structure Classification
    Initial Source

    Streptomyces verticillus
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 255 mg/mL (Need ultrasonic and warming)

    DMSO : 41.67 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL; Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL; Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL; Clear solution; Need ultrasonic and warming and heat to 60°C

    • Protocol 2

      Add each solvent one by one:  Saline

      Solubility: 100 mg/mL; Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.62%

    SDS (418 KB)

    COA (249 KB) HNMR (355 KB) LCMS (100 KB)

    Handling Instructions (2659 KB)
    References
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    Bleomycin sulfate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Bleomycin9041-93-4DNA/RNA SynthesisAntibioticInhibitorinhibitorinhibit

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