Butylphthalide (Synonyms: 3-n-Butylphthalide; 3-Butylphthalide)

Name: Butylphthalide
Brand: MedChemExpress
SKU: HY-B0647
Rating: 5.0 (5 reviews)

Cat. No.: HY-B0647 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions

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Butylphthalide (3-n-Butylphthalide) is an active molecule against cerebral ischemia. It was originally isolated from celery species and has been shown to be effective in stroke animal models.

For research use only. We do not sell to patients.

Butylphthalide Chemical Structure

CAS No. : 6066-49-5

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 106	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 106	In-stock	Estimated Time of Arrival: December 31
Liquid
500 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
1 g	USD 115	In-stock	Estimated Time of Arrival: December 31
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10 g		Get quote

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Based on 5 publication(s) in Google Scholar

Other Forms of Butylphthalide:

(3S)-Butylphthalide Get quote
Butylphthalide-d₃ Get quote

4 Publications Citing Use of MCE Butylphthalide

: Butylphthalide purchased from MedChemExpress. Usage Cited in: Front Aging Neurosci. 2019 Feb 28;11:44. [Abstract]; The protein level of TNF-α, IL-1β, iNOS and COX2 is analyzed by Western Blot with or without the treatment of NBP and LPS.

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Biological Activity
Purity & Documentation
References
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Description

Butylphthalide (3-n-Butylphthalide) is an active molecule against cerebral ischemia. It was originally isolated from celery species and has been shown to be effective in stroke animal models.

Cellular Effect

Cell Line	Type	Value	Description	References
Platelet	IC₅₀	> 1 mM Compound: NBP	Inhibition of adenosine diphosphate-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method Inhibition of adenosine diphosphate-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method	[PMID: 22827516]
Platelet	IC₅₀	0.48 mM Compound: NBP	Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method	[PMID: 22827516]
Platelet	IC₅₀	0.74 mM Compound: NBP	Antiplatelet activity in rabbit platelet-rich plasma assessed as inhibition of ADP-induced platelet aggregation preincubated for 5 mins followed by addition of ADP measured within 5 mins by Borns turbidimetric method Antiplatelet activity in rabbit platelet-rich plasma assessed as inhibition of ADP-induced platelet aggregation preincubated for 5 mins followed by addition of ADP measured within 5 mins by Borns turbidimetric method	[PMID: 27031213]

In Vivo

Butylphthalide (160mg/kg, i.g) improves learning and memory deficits in SAMP8 mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Old male SAMP8 mice and SAMR1 mice^[3]
Dosage:	40 mg/kg, 80 mg/kg, 160 mg/kg
Administration:	Orally daily, 60 days
Result:	Shorted escape latency time on day 7. Improved SAMP8 mice learning deficits.

Clinical Trial

Molecular Weight

190.24

Formula

C₁₂H₁₄O₂

CAS No.

6066-49-5

Appearance

Liquid (Density: 1.0527 g/cm³)

Color

Colorless to light yellow

SMILES

O=C1OC(CCCC)C2=C1C=CC=C2

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (1314.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2565 mL	26.2826 mL	52.5652 mL
5 mM	1.0513 mL	5.2565 mL	10.5130 mL
10 mM	0.5257 mL	2.6283 mL	5.2565 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 13.33 mg/mL (70.07 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (253 KB) HNMR (190 KB) LCMS (377 KB)

Handling Instructions (2659 KB)

References

[1]. Cui LY, et al. Ninety-day administration of dl-3-n-butylphthalide for acute ischemic stroke: a randomized, double-blind trial. Chin Med J (Engl). 2013;126(18):3405-10. [Content Brief]

[2]. Zhao W, et al. 3-N-butylphthalide improves neuronal morphology after chronic cerebral ischemia. Neural Regen Res. 2014 Apr 1;9(7):719-26. [Content Brief]

[3]. Wang X, et al. Design, synthesis and biological evaluation of hydrogen sulfide releasing derivatives of 3-n-butylphthalide as potential antiplatelet and antithrombotic agents. Org Biomol Chem. 2014 Aug 21;12(31):5995-6004. [Content Brief]

[4]. Wang F, et al. Improvement of cognitive deficits in SAMP8 mice by 3-n-butylphthalide. Neurol Res. 2014 Mar;36(3):224-33. [Content Brief]

[5]. Chen XQ, Qiu K, Liu H, He Q, Bai JH, Lu W. Application and prospects of butylphthalide for the treatment of neurologic diseases. Chin Med J (Engl). 2019;132(12):1467-1477. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.2565 mL	26.2826 mL	52.5652 mL	131.4129 mL
	5 mM	1.0513 mL	5.2565 mL	10.5130 mL	26.2826 mL
	10 mM	0.5257 mL	2.6283 mL	5.2565 mL	13.1413 mL
	15 mM	0.3504 mL	1.7522 mL	3.5043 mL	8.7609 mL
	20 mM	0.2628 mL	1.3141 mL	2.6283 mL	6.5706 mL
	25 mM	0.2103 mL	1.0513 mL	2.1026 mL	5.2565 mL
	30 mM	0.1752 mL	0.8761 mL	1.7522 mL	4.3804 mL
	40 mM	0.1314 mL	0.6571 mL	1.3141 mL	3.2853 mL
	50 mM	0.1051 mL	0.5257 mL	1.0513 mL	2.6283 mL
	60 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	80 mM	0.0657 mL	0.3285 mL	0.6571 mL	1.6427 mL
	100 mM	0.0526 mL	0.2628 mL	0.5257 mL	1.3141 mL

Butylphthalide Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Butylphthalide6066-49-53-n-Butylphthalide 3-ButylphthalideOthersInhibitorinhibitorinhibit

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Butylphthalide (Synonyms: 3-n-Butylphthalide; 3-Butylphthalide)

Customer Review

Other Forms of Butylphthalide:

Butylphthalide Related Antibodies

4 Publications Citing Use of MCE Butylphthalide

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Butylphthalide Related Classifications

Keywords:

Your Recently Viewed Products:

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