2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. Calycosin-7-O-β-D-glucoside

Calycosin-7-O-β-D-glucoside  (Synonyms: Calycosin-7-glucoside)

Cat. No.: HY-N0520 Purity: 98.86%
COA Handling Instructions

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Calycosin-7-O-β-D-glucoside is an isoflavone isolated from Astragali Radix. Calycosin-7-O-β-D-glucoside has variety of biological activities, such as neuroprotective, cardioprotection, anti-inflammation, and antioxidative stress effects.

For research use only. We do not sell to patients.

Calycosin-7-O-β-D-glucoside Chemical Structure

Calycosin-7-O-β-D-glucoside Chemical Structure

CAS No. : 20633-67-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 In-stock
Solution
10 mM * 1 mL in DMSO USD 77 In-stock
Solid
1 mg USD 28 In-stock
5 mg USD 70 In-stock
10 mg USD 110 In-stock
50 mg USD 350 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Calycosin-7-O-β-D-glucoside:

Calycosin-7-O-β-D-glucoside Related Antibodies

Top Publications Citing Use of Products
Proliferation Assay

    Calycosin-7-O-β-D-glucoside purchased from MedChemExpress. Usage Cited in: Chem Biol Interact. 2023 Feb 20;110411.  [Abstract]

    Calycosin-7-O-β-D-glucoside (CG; 0-1000 μM; 24 h) dose-dependent inhibits the proliferation of Huh-7 and HepG2 cells, with IC50 of 25.49 μM in Huh-7 cells and 18.39 μM in HepG2 cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Calycosin-7-O-β-D-glucoside is an isoflavone isolated from Astragali Radix. Calycosin-7-O-β-D-glucoside has variety of biological activities, such as neuroprotective, cardioprotection, anti-inflammation, and antioxidative stress effects[1][2].

    In Vitro

    Calycosin-7-O-β-D-glucoside (2 μM; 6 hours) remarkably inhibits the expression and activities of MMPs, and secures the expression of cav-1 and tight junction proteins in the microvessels isolated from ischemic rat cortex[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Calycosin-7-O-β-D-glucoside Related Antibodies
    In Vivo

    Calycosin-7-O-β-D-glucoside (intraperitoneal injection; 26.8 mg/kg; 14 days) significantly reduces infarct volume, histological damage and BBB permeability in the in vivo MCAO ischemia-reperfusion rat model[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Middle cerebral artery occlusion (MCAO) male adult Sprague-Daweley rats[1]
    Dosage: 26.8 mg/kg
    Administration: Intraperitoneal injection; 26.8 mg/kg; 14 days
    Result: Exhibited neuroprotective effects in rats.
    Molecular Weight

    446.40

    Formula

    C22H22O10
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1C2=CC=C(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)C=C2OC=C1C4=CC(O)=C(OC)C=C4
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (224.01 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2401 mL 11.2007 mL 22.4014 mL
    5 mM 0.4480 mL 2.2401 mL 4.4803 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.60 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (5.60 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.86%

    COA (249 KB) HNMR (285 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2401 mL 11.2007 mL 22.4014 mL 56.0036 mL
    5 mM 0.4480 mL 2.2401 mL 4.4803 mL 11.2007 mL
    10 mM 0.2240 mL 1.1201 mL 2.2401 mL 5.6004 mL
    15 mM 0.1493 mL 0.7467 mL 1.4934 mL 3.7336 mL
    20 mM 0.1120 mL 0.5600 mL 1.1201 mL 2.8002 mL
    25 mM 0.0896 mL 0.4480 mL 0.8961 mL 2.2401 mL
    30 mM 0.0747 mL 0.3734 mL 0.7467 mL 1.8668 mL
    40 mM 0.0560 mL 0.2800 mL 0.5600 mL 1.4001 mL
    50 mM 0.0448 mL 0.2240 mL 0.4480 mL 1.1201 mL
    60 mM 0.0373 mL 0.1867 mL 0.3734 mL 0.9334 mL
    80 mM 0.0280 mL 0.1400 mL 0.2800 mL 0.7000 mL
    100 mM 0.0224 mL 0.1120 mL 0.2240 mL 0.5600 mL
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    Calycosin-7-O-β-D-glucoside Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Calycosin-7-O-β-D-glucoside20633-67-4Calycosin-7-glucosideReactive Oxygen Speciescardioprotectionanti-inflammationantioxidative stressneuroprotectiveisoflavoneInhibitorinhibitorinhibit

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