1. Cell Cycle/DNA Damage
  2. DNA Alkylator/Crosslinker
  3. Carmustine

Carmustine is an antitumor chemotherapeutic agent, which works by akylating DNA and RNA.

For research use only. We do not sell to patients.

Carmustine Chemical Structure

Carmustine Chemical Structure

CAS No. : 154-93-8

Size Price Stock Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 33 In-stock
Solution
10 mM * 1 mL in DMSO USD 33 In-stock
Solid or liquid
5 mg USD 30 In-stock
10 mg USD 50 In-stock
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100 mg USD 135 In-stock
500 mg USD 370 In-stock
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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of Carmustine:

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Carmustine is an antitumor chemotherapeutic agent, which works by akylating DNA and RNA.

IC50 & Target

DNA Alkylator[1]

Cellular Effect
Cell Line Type Value Description References
A 172 IC50
18 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of A172Mer- cell line of central nervous system (CNS) using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of A172Mer- cell line of central nervous system (CNS) using the MTT Cytotoxicity Assay
[PMID: 2391696]
A-427 IC50
8 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of A427Mer- cell line of lung using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of A427Mer- cell line of lung using the MTT Cytotoxicity Assay
[PMID: 2391696]
A498 IC50
110 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of A498Rem- renal cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of A498Rem- renal cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
A549 IC50
90 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of A549Rem- cell line of lung using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of A549Rem- cell line of lung using the MTT Cytotoxicity Assay
[PMID: 2391696]
Astrocyte EC50
54.8 μM
Compound: 1, BCNU, Carmustine
Cytotoxicity against Rattus norvegicus Sprague-Dawley (rat) astrocytes after 4 days by cresylecth violet-staining method
Cytotoxicity against Rattus norvegicus Sprague-Dawley (rat) astrocytes after 4 days by cresylecth violet-staining method
10.1007/s00044-010-9356-8
C6 EC50
4.8 μM
Compound: 1, BCNU, Carmustine
Cytotoxicity against Rattus norvegicus (rat) C6 cells after 4 days by cresylecth violet-staining method
Cytotoxicity against Rattus norvegicus (rat) C6 cells after 4 days by cresylecth violet-staining method
10.1007/s00044-010-9356-8
CCD 19Lu IC50
300 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of CCD-19Lu fibroblasts cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of CCD-19Lu fibroblasts cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
DOD-1 IC50
37 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of DOD-1 breast cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of DOD-1 breast cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
HL-60 IC50
30.5 μM
Compound: BCNU
Cytotoxicity against Homo sapiens (human) HL60 cells after 96 hr by MTT assay
Cytotoxicity against Homo sapiens (human) HL60 cells after 96 hr by MTT assay
10.1007/s00044-011-9778-y
L1210 ED50
4 μM
Compound: BCNU
Cytotoxicity against mouse L1210 cells after 72 hrs by coulter counter analysis
Cytotoxicity against mouse L1210 cells after 72 hrs by coulter counter analysis
[PMID: 563461]
LOX IMVI IC50
7 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of LOX melanoma cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of LOX melanoma cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
Malme-3M IC50
20 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of MALME-3M melanoma cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of MALME-3M melanoma cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
MCF7 IC50
4.5 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of MCF-7 breast cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of MCF-7 breast cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
NCH89 IC50
615 μM
Compound: Carmustine
Antiproliferative activity against human NCH-89 cells assessed as BrdU incorporation after 48 hrs
Antiproliferative activity against human NCH-89 cells assessed as BrdU incorporation after 48 hrs
[PMID: 20329733]
NCI/ADR-RES IC50
20 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of MCF-7ADR breast cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of MCF-7ADR breast cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
NCI-H125 IC50
9 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of H125 cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of H125 cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
[PMID: 2391696]
NCI-H23 IC50
5 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of H23 cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of H23 cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
[PMID: 2391696]
NCI-H322M IC50
60 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of H322M cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of H322M cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
[PMID: 2391696]
NCI-H358 IC50
98 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of H358M cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of H358M cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
[PMID: 2391696]
NCI-H460 IC50
80 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of H460 cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of H460 cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
[PMID: 2391696]
NCI-H520 IC50
20 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of H522 cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of H522 cell line of lung using the Microculture Tetrazolium (MTT) Cytotoxicity Assay
[PMID: 2391696]
OVCAR-3 IC50
15 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of OVCAR-3 ovarian cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of OVCAR-3 ovarian cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
OVCAR-4 IC50
18 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of OVCAR-4 ovarian cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of OVCAR-4 ovarian cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
SF-126 IC50
20 μM
Compound: BCNU
Cytotoxicity against human SF126 cells expressing low level of AGT assessed as cell survival after 24 hrs by CCK8 assay
Cytotoxicity against human SF126 cells expressing low level of AGT assessed as cell survival after 24 hrs by CCK8 assay
[PMID: 27041398]
SF-126 IC50
25 μM
Compound: BCNU
Cytotoxicity against human SF126 cells expressing low level of AGT assessed as cell survival after 24 hrs by CCK8 assay in presence of O6-BG
Cytotoxicity against human SF126 cells expressing low level of AGT assessed as cell survival after 24 hrs by CCK8 assay in presence of O6-BG
[PMID: 27041398]
SF-126 IC50
55 μM
Compound: BCNU
Cytotoxicity against AGT-deficient human SF126 cells assessed as reduction in cell viability pretreated with O6-BG for 2 hrs followed by addition of compound measured after 24 hrs by CCK8 assay
Cytotoxicity against AGT-deficient human SF126 cells assessed as reduction in cell viability pretreated with O6-BG for 2 hrs followed by addition of compound measured after 24 hrs by CCK8 assay
[PMID: 28197307]
SF-126 IC50
85 μM
Compound: BCNU
Cytotoxicity against AGT-deficient human SF126 cells assessed as reduction in cell viability measured after 24 hrs by CCK8 assay
Cytotoxicity against AGT-deficient human SF126 cells assessed as reduction in cell viability measured after 24 hrs by CCK8 assay
[PMID: 28197307]
SK-MEL-5 IC50
27 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of SK-MEL-5 melanoma cell line using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of SK-MEL-5 melanoma cell line using the MTT Cytotoxicity Assay
[PMID: 2391696]
TE-671 IC50
25 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of TE-671 cell line of central nervous system (CNS)using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of TE-671 cell line of central nervous system (CNS)using the MTT Cytotoxicity Assay
[PMID: 2391696]
U-251 IC50
15 μM
Compound: BCNU
Evaluated for the inhibitory concentration required to cause growth inhibition of U251 cell line of central nervous system (CNS) using the MTT Cytotoxicity Assay
Evaluated for the inhibitory concentration required to cause growth inhibition of U251 cell line of central nervous system (CNS) using the MTT Cytotoxicity Assay
[PMID: 2391696]
U-87MG ATCC IC50
1.42 μM
Compound: BCNU
In vitro inhibitory concentration against growth of U87MG glioblastoma cell line in clonogenic survival assay after 24 hr
In vitro inhibitory concentration against growth of U87MG glioblastoma cell line in clonogenic survival assay after 24 hr
[PMID: 15603970]
U-87MG ATCC IC50
3.9 μg/mL
Compound: BCNU
Cytotoxicity against Homo sapiens (human) U87 cells by MTT assay
Cytotoxicity against Homo sapiens (human) U87 cells by MTT assay
10.1007/s00044-012-0249-x
U-87MG ATCC IC50
3.9 μg/mL
Compound: Carmustine
Antiproliferative activity against human U87 cells assessed as reduction in cell viability by MTT assay
Antiproliferative activity against human U87 cells assessed as reduction in cell viability by MTT assay
[PMID: 33276991]
U-937 IC50
12.3 μM
Compound: BCNU
Cytotoxicity against Homo sapiens (human) U937 cells after 96 hr by MTT assay
Cytotoxicity against Homo sapiens (human) U937 cells after 96 hr by MTT assay
10.1007/s00044-011-9778-y
In Vitro

Carmustine is an antitumor chemotherapeutic agent. Carmustine (8, 80, and 800 μM) decreases N-acetyltransferase (NAT) activities for 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) in rat glial tumor cytosol and intact cells. In rat glial tumor cells, the DNA-AF adduct increases, and carmustine decreases the formation of DNA-AF adduct[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Carmustine (BCNU; 25 mg/kg, i.p.) causes higher levels of the rhe ratio of liver weight to body weight and plasma conjugated bilirubin, and lower biliary flow, oxidised glutation levels (GSSG) and reduced glutation (GSH)/GSSG values compared with control rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

214.05

Formula

C5H9Cl2N3O2

CAS No.
Appearance

<30°C Solid,>30°C Liquid

Color

White to yellow

SMILES

O=C(NCCCl)N(CCCl)N=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (467.18 mM; Need ultrasonic)

DMSO : ≥ 35 mg/mL (163.51 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6718 mL 23.3590 mL 46.7181 mL
5 mM 0.9344 mL 4.6718 mL 9.3436 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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Volume (final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (9.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (9.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (467.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.87%

References
Kinase Assay
[1]

The determination of Acetyl-CoAdependent N-acetylation of 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) are performed. Incubation mixtures in the assay system consists of a total volume of 90 μL: glial tumor cells cytosols, diluted as required, in 50 μL of lysis buffer (20 mM Tris/HCl, pH 7.5, 1 mM DTT and 1 mM EDTA), 20 μL of an Acetyl-CoA recycling mixture of 50 mM Tris-HCl (pH7.5), 0.2 mM EDTA, 2 mM DTT, 15 mM acetylcamitine, 2U/mL carnitine acetyltransferase, and AF or PABA at specific concentrations. The reactions are started by addition of 20 μL of Acetyl-CoA. The control reactions have 20 μL distilled water in place of Acetyl-CoA. For the single point activity measurements, the final concentration of AF or PABA is 0.1 mM and AcCoA is 0.5 mM. The reaction mixtures with or without specific concentrations of Carmustine and lomustine are incubated at 37°C for 10 min and stopped with 50 μL of 20% trichloroacetic acid for the PABA reactions, and 100 μL of acetonitrile for the AF reactions. All of the reactions (experiments and controls) are run in triplicate[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Rats[2]
Individual rats are weighted prior to enter the study; their weights are recorded, and they are randomLy assigned to four groups. Group I (saline group); This group consists of 12 rats. These rats are injected with 2 mL/kg of saline intraperitoneally (IP) 48 h before the study, being included by the study 48 h later. Group II (corn oil group) consists of 15 rats. These rats are injected with 2 mL/kg of corn oil (vehicle) IP 48 h before the study. Group III (Carmustine group) consists of 16 rats. These rats are injected with 1 mL per day of saline IP, administered at the same hour of the day as a single-dose for 3 days. Twelve hours after the first dose of saline, corn oil 2 mL/kg + Carmustine 25 mg/kg IP are injected, and the rats are included in the study 48 h after the administration of corn oil + Carmustine. Group IV (trimetazidine group) consists of 12 rats. These rats are injected with 2.5 mg/kg per day of trimetazidine (TMZ) IP, administered at the same hour of the day as a single-dose for 3 days. 12 h after the first dose of TMZ, corn oil 2 mL/kg + Carmustine 25 mg/kg IP are injected, and the rats are included in the study 48 h after the administration of corn oil + Carmustine[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.6718 mL 23.3590 mL 46.7181 mL 116.7951 mL
5 mM 0.9344 mL 4.6718 mL 9.3436 mL 23.3590 mL
10 mM 0.4672 mL 2.3359 mL 4.6718 mL 11.6795 mL
15 mM 0.3115 mL 1.5573 mL 3.1145 mL 7.7863 mL
20 mM 0.2336 mL 1.1680 mL 2.3359 mL 5.8398 mL
25 mM 0.1869 mL 0.9344 mL 1.8687 mL 4.6718 mL
30 mM 0.1557 mL 0.7786 mL 1.5573 mL 3.8932 mL
40 mM 0.1168 mL 0.5840 mL 1.1680 mL 2.9199 mL
50 mM 0.0934 mL 0.4672 mL 0.9344 mL 2.3359 mL
60 mM 0.0779 mL 0.3893 mL 0.7786 mL 1.9466 mL
80 mM 0.0584 mL 0.2920 mL 0.5840 mL 1.4599 mL
100 mM 0.0467 mL 0.2336 mL 0.4672 mL 1.1680 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Carmustine
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