2. GPCR/G Protein Metabolic Enzyme/Protease
  3. G protein-coupled Bile Acid Receptor 1 Endogenous Metabolite
  4. Deoxycholic acid sodium salt

Deoxycholic acid sodium salt  (Synonyms: Sodium deoxycholate)

Cat. No.: HY-N0593A Purity: 99.89%
SDS COA Handling Instructions

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Deoxycholic acid sodium salt (sodium deoxycholate), a bile acid, is a by-product of intestinal metabolism, that activates the G protein-coupled bile acid receptorTGR5.

For research use only. We do not sell to patients.

Deoxycholic acid sodium salt Chemical Structure

Deoxycholic acid sodium salt Chemical Structure

CAS No. : 302-95-4

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100 mg USD 50 In-stock
500 mg USD 70 In-stock
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Based on 14 publication(s) in Google Scholar

Other Forms of Deoxycholic acid sodium salt:

Deoxycholic acid sodium salt Related Antibodies

Top Publications Citing Use of Products

    Deoxycholic acid sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.  [Abstract]

    The expression of CDX2, KLF4, HNF4α, and cadherin 17 is increased upon stimulation with DCA in a dose-dependent manner.

    Deoxycholic acid sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.  [Abstract]

    Western blotting results indicated that the inhibition of SOX2 in bile acid-treated AGS cells is partially rescued in miR-21 knockdown AGS cells.

    Deoxycholic acid sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.  [Abstract]

    The expression of CDX2, KLF4, HNF4α, and cadherin 17 is increased upon stimulation with DCA in a dose-dependent manner.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Deoxycholic acid sodium salt (sodium deoxycholate), a bile acid, is a by-product of intestinal metabolism, that activates the G protein-coupled bile acid receptorTGR5[1][2].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    In Vitro

    Deoxycholic acid sodium hydrate (DCA) (100 μM) induces the production of gastric cancer cell line MGC803 resistant to acidified bile acids and enhances their survival and proliferation activity under bile acid stress[2].
    Deoxycholic acid sodium hydrate (DCA) (100 μM)-induced resistant cells shows altered morphology, significantly elevated telomerase activity, better cell viability and reduces apoptosis compared to normal MGC803 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Deoxycholic acid sodium salt Related Antibodies
    Clinical Trial
    Molecular Weight

    414.55

    Formula

    C24H39NaO4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]1([C@@]2([H])[C@H](C)CCC(O[Na])=O)[C@](CC2)([H])[C@@](CC[C@@]3([H])[C@@]4(CC[C@@H](O)C3)C)([H])[C@]4([H])C[C@@H]1O
    Structure Classification
    Initial Source

    intestinal bacteria
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 333.33 mg/mL (804.08 mM; Need ultrasonic)

    DMSO : 4 mg/mL (9.65 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4123 mL 12.0613 mL 24.1225 mL
    5 mM 0.4825 mL 2.4123 mL 4.8245 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.62 mg/mL (1.50 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.62 mg/mL (1.50 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (120.61 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.89%

    SDS (453 KB)

    COA (248 KB) HNMR (268 KB) RP-HPLC (217 KB) MS (81 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented with 10% fetal calf serum and 100 U/mL Penicillin and 100 mg/mL Streptomycin. To generate MGC803-resistant cells, the pH value of the MGC803 culture medium is adjusted to the experimental conditions using the hydrochloric acid (A). The bile acids GCDA and Deoxycholic acid are diluted to optimal working concentrations of 100 μM (B) with culture medium, and the overall pH (A+B) is adjusted to pH 5.5, simulating the gastric environment. Initially, MGC803 cells are chronically exposed to acidified medium with bile acids (A+B) for 10 min every 24 h. The experimental time and conditions are optimized in our preliminary experiments, which show that 10 min is enough and does not result in cell damage. This procedure is repeated and it takes 60 weeks for the MGC803 cells to survive and proliferate under the exposure of A+B for 120 min. Control untreated cells are cultured in neutral RPMI medium at pH 7.4 in parallel to the resistant cells for 60 weeks. The morphological changes in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and 60 weeks[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 2.4123 mL 12.0613 mL 24.1225 mL 60.3064 mL
    5 mM 0.4825 mL 2.4123 mL 4.8245 mL 12.0613 mL
    H2O 10 mM 0.2412 mL 1.2061 mL 2.4123 mL 6.0306 mL
    15 mM 0.1608 mL 0.8041 mL 1.6082 mL 4.0204 mL
    20 mM 0.1206 mL 0.6031 mL 1.2061 mL 3.0153 mL
    25 mM 0.0965 mL 0.4825 mL 0.9649 mL 2.4123 mL
    30 mM 0.0804 mL 0.4020 mL 0.8041 mL 2.0102 mL
    40 mM 0.0603 mL 0.3015 mL 0.6031 mL 1.5077 mL
    50 mM 0.0482 mL 0.2412 mL 0.4825 mL 1.2061 mL
    60 mM 0.0402 mL 0.2010 mL 0.4020 mL 1.0051 mL
    80 mM 0.0302 mL 0.1508 mL 0.3015 mL 0.7538 mL
    100 mM 0.0241 mL 0.1206 mL 0.2412 mL 0.6031 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Deoxycholic acid sodium salt Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Deoxycholic acid302-95-4Sodium deoxycholateG protein-coupled Bile Acid Receptor 1Endogenous MetaboliteG-protein coupled receptor 19GPCR19TGR5GPBAR1bile acidG protein-coupled bile acid receptorInhibitorinhibitorinhibit

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