2. Anti-infection
  3. HIV Reverse Transcriptase
  4. Didanosine

Didanosine  (Synonyms: 2',3'-Dideoxyinosine; ddI)

Cat. No.: HY-B0249 Purity: 99.94%
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Didanosine (2',3'-Dideoxyinosine; ddI) is a a potent and orally active dideoxynucleoside analogue, and also is a potent nucleoside reverse transcriptase inhibitor. Didanosine shows antiretroviral activity for HIV.

For research use only. We do not sell to patients.

Didanosine Chemical Structure

Didanosine Chemical Structure

CAS No. : 69655-05-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 In-stock
Solution
10 mM * 1 mL in DMSO USD 77 In-stock
Solid
5 mg USD 45 In-stock
10 mg USD 70 In-stock
50 mg USD 140 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Didanosine:

Didanosine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Didanosine

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Didanosine (2',3'-Dideoxyinosine; ddI) is a a potent and orally active dideoxynucleoside analogue, and also is a potent nucleoside reverse transcriptase inhibitor. Didanosine shows antiretroviral activity for HIV[1][2][3].

In Vitro

Didanosine is converted by cellular enzymes to the active antiviral metabolite dideoxyadenosine triphosphate (dd-ATP) and the intracellular half life of dd-ATP varies from 8-24 hours[1].
Didanosine shows antiretroviral activity with IC50 value of 0.24-0.6 mg/L for HIV infection[3].
Didanosine (5, 10, 50, 100 ug/ml; 24, 48 h) shows no significant inhibition of cell proliferation in IEC-6 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Didanosine Related Antibodies

Cell Proliferation Assay[2]

Cell Line: IEC-6 cells
Concentration: 5, 10, 50, 100 ug/ml
Incubation Time: 24, 48 h
Result: Did not show any significant inhibition of cell proliferation at either 24 h or 48 h.

Apoptosis Analysis[2]

Cell Line: IEC-6 cells
Concentration: 100 ug/ml
Incubation Time: 24 h
Result: Induced apoptosis with the apoptosis rates of 4.7% to 7.4%.
In Vivo

Didanosine (100, 150 mg/kg; p.o.; daily for 7 days) decreases the length of duodenal and in jejunal villus in mouse[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 30-40 g, male swiss mice[2]
Dosage: 100, 150 mg/kg
Administration: P.o.; daily for 7 days
Result: Caused significant reductions in duodenal and in jejunal villus length and significantly decreased ileal crypt depth.
Clinical Trial
Molecular Weight

236.23

Formula

C10H12N4O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1CC[C@H](N2C3=C(N=C2)C(O)=NC=N3)O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (264.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.2332 mL 21.1658 mL 42.3316 mL
5 mM 0.8466 mL 4.2332 mL 8.4663 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.2332 mL 21.1658 mL 42.3316 mL 105.8291 mL
5 mM 0.8466 mL 4.2332 mL 8.4663 mL 21.1658 mL
10 mM 0.4233 mL 2.1166 mL 4.2332 mL 10.5829 mL
15 mM 0.2822 mL 1.4111 mL 2.8221 mL 7.0553 mL
20 mM 0.2117 mL 1.0583 mL 2.1166 mL 5.2915 mL
25 mM 0.1693 mL 0.8466 mL 1.6933 mL 4.2332 mL
30 mM 0.1411 mL 0.7055 mL 1.4111 mL 3.5276 mL
40 mM 0.1058 mL 0.5291 mL 1.0583 mL 2.6457 mL
50 mM 0.0847 mL 0.4233 mL 0.8466 mL 2.1166 mL
60 mM 0.0706 mL 0.3528 mL 0.7055 mL 1.7638 mL
80 mM 0.0529 mL 0.2646 mL 0.5291 mL 1.3229 mL
100 mM 0.0423 mL 0.2117 mL 0.4233 mL 1.0583 mL
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Didanosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Didanosine69655-05-62',3'-Dideoxyinosine ddIHIVReverse TranscriptaseHuman immunodeficiency virusantiretroviralapoptosisIEC-6 cellsorally activeInhibitorinhibitorinhibit

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