EML4-ALK kinase inhibitor 1

Name: EML4-ALK kinase inhibitor 1
Brand: MedChemExpress
SKU: HY-111752
Rating: 4.5 (1 reviews)

Cat. No.: HY-111752 Purity: 98.02%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

EML4-ALK kinase inhibitor 1 is a potent orally active inhibitor of echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK), with an IC₅₀ of 1 nM.

For research use only. We do not sell to patients.

EML4-ALK kinase inhibitor 1 Chemical Structure

CAS No. : 1373409-08-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 473	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 473	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 370	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 890	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1220	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

EML4-ALK kinase inhibitor 1 is a potent orally active inhibitor of echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK), with an IC₅₀ of 1 nM^[1].

IC₅₀ & Target

IC50: 1 nM (EML4-ALK)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
BaF3	IC₅₀	1 nM Compound: 12c	Inhibition of EML4-fused ALK variant 1 (unknown origin) expressed in mouse BAF3 cells using peptide substrate measured after 1 hr by HTRF assay Inhibition of EML4-fused ALK variant 1 (unknown origin) expressed in mouse BAF3 cells using peptide substrate measured after 1 hr by HTRF assay	[PMID: 30878193]
BaF3	IC₅₀	9.7 nM Compound: 12c	Antiproliferative activity against mouse BAF3 cells expressing EML4-fused ALK variant 1 assessed as reduction in cell viability measured after 2 days by alamar blue assay Antiproliferative activity against mouse BAF3 cells expressing EML4-fused ALK variant 1 assessed as reduction in cell viability measured after 2 days by alamar blue assay	[PMID: 30878193]

Molecular Weight

580.76

Formula

C₃₁H₄₈N₈O₃

CAS No.

1373409-08-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

CO[C@H]1CC[C@H](NC2=C(CC)N=C(C(N)=O)C(NC3=CC(OC)=C(N4CCC(N5CCN(C)CC5)CC4)C=C3)=N2)CC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 110 mg/mL (189.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7219 mL	8.6094 mL	17.2188 mL
5 mM	0.3444 mL	1.7219 mL	3.4438 mL
10 mM	0.1722 mL	0.8609 mL	1.7219 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5.5 mg/mL (9.47 mM); Clear solution

This protocol yields a clear solution of ≥ 5.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (55.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5.5 mg/mL (9.47 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 5.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (55.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.02%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (248 KB) RP-HPLC (256 KB) LCMS (101 KB)

Handling Instructions (2659 KB)

References

[1]. Iikubo K, et al. Synthesis and structure-activity relationships of pyrazine-2-carboxamide derivatives as novel echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) inhibitors. Bioorg Med Chem. 2019 Apr 15;27(8):1683-169 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7219 mL	8.6094 mL	17.2188 mL	43.0470 mL
	5 mM	0.3444 mL	1.7219 mL	3.4438 mL	8.6094 mL
	10 mM	0.1722 mL	0.8609 mL	1.7219 mL	4.3047 mL
	15 mM	0.1148 mL	0.5740 mL	1.1479 mL	2.8698 mL
	20 mM	0.0861 mL	0.4305 mL	0.8609 mL	2.1524 mL
	25 mM	0.0689 mL	0.3444 mL	0.6888 mL	1.7219 mL
	30 mM	0.0574 mL	0.2870 mL	0.5740 mL	1.4349 mL
	40 mM	0.0430 mL	0.2152 mL	0.4305 mL	1.0762 mL
	50 mM	0.0344 mL	0.1722 mL	0.3444 mL	0.8609 mL
	60 mM	0.0287 mL	0.1435 mL	0.2870 mL	0.7175 mL
	80 mM	0.0215 mL	0.1076 mL	0.2152 mL	0.5381 mL
	100 mM	0.0172 mL	0.0861 mL	0.1722 mL	0.4305 mL