2. Apoptosis NF-κB
  3. Apoptosis NF-κB
  4. Eleutheroside E

Eleutheroside E is an important component of ginseng that can be taken orally. Eleutheroside E has anti-inflammatory and antioxidant properties, and it helps reduce apoptosis in heart cells caused by hypoxia-reoxygenation (H/R) damage. Eleutheroside E can improve type 2 diabetes, enhance cognitive function, and has neuroprotective effects.

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Eleutheroside E Chemical Structure

Eleutheroside E Chemical Structure

CAS No. : 39432-56-9

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Eleutheroside E:

Eleutheroside E Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Eleutheroside E

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Description

Eleutheroside E is an important component of ginseng that can be taken orally. Eleutheroside E has anti-inflammatory and antioxidant properties, and it helps reduce apoptosis in heart cells caused by hypoxia-reoxygenation (H/R) damage. Eleutheroside E can improve type 2 diabetes, enhance cognitive function, and has neuroprotective effects[1][2][3][4].

In Vitro

Eleutheroside E (10 μM, 24 h) increases insulin-induced glucose uptake in C2C12 myotubes and improves the inhibition of glucose uptake by TNF-α in 3T3-L1 adipocytes[1].
Eleutheroside E (0-100 μM, 3 h) enhances cell viability in H9c2 cells subjected to H/R injury[2].
Eleutheroside E (0-100 μM, 12 h) has a protective effect against apoptosis in H9c2 cells induced by H/R injury[2].
Eleutheroside E (100 μM, 12 h) boosts antioxidant capacity in H9c2 cells[2].
Eleutheroside E (100 μM, 12 h) blocks the MAPK pathway and inhibits the activation of NF-κB[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Eleutheroside E Related Antibodies

Cell Viability Assay[2]

Cell Line: H9c2, H9c2 (H/r)
Concentration: 0, 30, 60, 100 μM
Incubation Time: 3 h
Result: Enhanced the cell viability of H9c2 (H/r) cells in a dose-dependent manner and had no effect on normal cells.

Western Blot Analysis[2]

Cell Line: H9c2 (H/r)
Concentration: 100 μM
Incubation Time: 12 h
Result: Reduced the expression of Bax, caspase-3 and caspase-9, and enhanced the expression of Bcl-2. Reduced the expression of Cyt-Cyto-C, reduced the levels of LDH and HMGB1 induced by H2O2, and inhibited the phosphorylation of ERK, JNK and p38.
In Vivo

Eleutheroside E (0.003%, oral, daily, for five weeks) improves liver glucose metabolism in obese type 2 diabetes mice by upregulating glycolysis and downregulating gluconeogenesis[1].
Eleutheroside E (15-60 mg/kg, oral, once daily, three weeks) alleviates arthritis in a collagen-induced arthritis mouse model[3].
Eleutheroside E (50 mg/kg, oral, once daily, four weeks) prevents cognitive deficits caused by radiation in mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: db/db mice[1]
Dosage: Containing 0.003% of the diet; 5 weeks
Administration: Oral
Result: Improved blood lipids, significantly reduced blood sugar and serum insulin levels, induced HOMA-IR, increased insulin sensitivity, and protected pancreatic α and β cells from diabetic damage.
Animal Model: Collageninduced arthritis (CIA) mice[3]
Dosage: 15, 30, 60 mg/kg; daily; 3 weeks
Administration: Oral
Result: Reduced mean arthritis score and arthritis incidence, reduced inflammatory cell infiltration, mesenchymal formation, cartilage damage and bone erosion in CIA mice, and decreased TNF-α and IL-6.
Animal Model: Mice irradiated with 60Co-γ rays[4]
Dosage: 50 mg/kg; daily; 4 weeks
Administration: Oral
Result: Improved cognitive and spatial memory impairments in irradiated mice, protected hippocampal neurons, and activated the PKA/CREB/BDNF signaling pathway.
Molecular Weight

742.72

Formula

C34H46O18
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

COC(C=C1[C@H]2[C@@](CO[C@@H]3C4=CC(OC)=C(O[C@@H]([C@@H]([C@@H](O)[C@@H]5O)O)O[C@@H]5CO)C(OC)=C4)([H])[C@]3([H])CO2)=C(C(OC)=C1)O[C@@H]([C@@H]([C@@H](O)[C@@H]6O)O)O[C@@H]6CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (134.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1 mg/mL (1.35 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3464 mL 6.7320 mL 13.4640 mL
5 mM 0.2693 mL 1.3464 mL 2.6928 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.37 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.37 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.58%

SDS (392 KB)

COA (257 KB) RP-HPLC (245 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.3464 mL 6.7320 mL 13.4640 mL 33.6601 mL
DMSO 5 mM 0.2693 mL 1.3464 mL 2.6928 mL 6.7320 mL
10 mM 0.1346 mL 0.6732 mL 1.3464 mL 3.3660 mL
15 mM 0.0898 mL 0.4488 mL 0.8976 mL 2.2440 mL
20 mM 0.0673 mL 0.3366 mL 0.6732 mL 1.6830 mL
25 mM 0.0539 mL 0.2693 mL 0.5386 mL 1.3464 mL
30 mM 0.0449 mL 0.2244 mL 0.4488 mL 1.1220 mL
40 mM 0.0337 mL 0.1683 mL 0.3366 mL 0.8415 mL
50 mM 0.0269 mL 0.1346 mL 0.2693 mL 0.6732 mL
60 mM 0.0224 mL 0.1122 mL 0.2244 mL 0.5610 mL
80 mM 0.0168 mL 0.0842 mL 0.1683 mL 0.4208 mL
100 mM 0.0135 mL 0.0673 mL 0.1346 mL 0.3366 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Eleutheroside E Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Eleutheroside E39432-56-9ApoptosisNF-κBNuclear factor-κBNuclear factor-kappaBAntioxidantanti-inflammatoryneuroprotectiveapoptoticInhibitorinhibitorinhibit

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