FDI-6

Name: FDI-6
Brand: MedChemExpress
SKU: HY-112721
Rating: 5.0 (2 reviews)

Cat. No.: HY-112721 Purity: 99.65%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

FDI-6 is an inhibitor of FOXM1. FDI-6 binds directly to FOXM1 protein, to displace FOXM1 from genomic targets in MCF-7 breast cancer cells, and induce concomitant transcriptional down-regulation.

For research use only. We do not sell to patients.

FDI-6 Chemical Structure

CAS No. : 313380-27-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 105	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 105	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 175	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE FDI-6

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Description

FDI-6 is an inhibitor of FOXM1. FDI-6 binds directly to FOXM1 protein, to displace FOXM1 from genomic targets in MCF-7 breast cancer cells, and induce concomitant transcriptional down-regulation.

IC₅₀ & Target

FOXM1^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	86.14 μM Compound: FDI6	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 22 to 70 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 22 to 70 hrs by CCK-8 assay	[PMID: 36334454]
HepG2	IC₅₀	70.5 μM Compound: FDI6	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth measured after 22 to 70 hrs by CCK-8 assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth measured after 22 to 70 hrs by CCK-8 assay	[PMID: 36334454]
MDA-MB-231	GI₅₀	25.6 μM Compound: FDI-6	Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition by MTT assay	[PMID: 34812040]
SK-OV-3	IC₅₀	51.36 μM Compound: FDI6	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth measured after 22 to 70 hrs by CCK-8 assay Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth measured after 22 to 70 hrs by CCK-8 assay	[PMID: 36334454]

In Vitro

FDI-6 is characterized in depth and is shown to bind directly to FOXM1 protein, to displace FOXM1 from genomic targets in MCF-7 breast cancer cells, and induce concomitant transcriptional down-regulation. MDA-MB-231 ER-negative breast and PEO-1 ovarian cancer cells are sensitive to FDI-6 in cell viability assays (GI₅₀=21.8 μM and 18.1 μM, respectively) and exhibit comparable down-regulation of CDC25B after a 3 h treatment with FDI-6. The transcription factor FOXM1 regulates a network of proliferation-associated genes critical to mitotic spindle assembly, chromosome segregation, and G₂/M transition, with depletion leading to cell cycle arrest. Importantly, aberrant up-regulation of FOXM1 has been shown to be a key driver of cancer progression and has been proposed as an initiating factor of oncogenesis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

437.43

Formula

C₁₉H₁₁F₄N₃OS₂

CAS No.

313380-27-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(N)C2=C(C(F)(F)F)C=C(C3=CC=CS3)N=C2S1)NC4=CC=C(F)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 41.67 mg/mL (95.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2861 mL	11.4304 mL	22.8608 mL
5 mM	0.4572 mL	2.2861 mL	4.5722 mL
10 mM	0.2286 mL	1.1430 mL	2.2861 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.72 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.65%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (249 KB) LCMS (148 KB)

Handling Instructions (2659 KB)

References

[1]. Gormally MV, et al. Suppression of the FOXM1 transcriptional programme via novel small molecule inhibition. Nat Commun. 2014 Nov 12;5:5165. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2861 mL	11.4304 mL	22.8608 mL	57.1520 mL
	5 mM	0.4572 mL	2.2861 mL	4.5722 mL	11.4304 mL
	10 mM	0.2286 mL	1.1430 mL	2.2861 mL	5.7152 mL
	15 mM	0.1524 mL	0.7620 mL	1.5241 mL	3.8101 mL
	20 mM	0.1143 mL	0.5715 mL	1.1430 mL	2.8576 mL
	25 mM	0.0914 mL	0.4572 mL	0.9144 mL	2.2861 mL
	30 mM	0.0762 mL	0.3810 mL	0.7620 mL	1.9051 mL
	40 mM	0.0572 mL	0.2858 mL	0.5715 mL	1.4288 mL
	50 mM	0.0457 mL	0.2286 mL	0.4572 mL	1.1430 mL
	60 mM	0.0381 mL	0.1905 mL	0.3810 mL	0.9525 mL
	80 mM	0.0286 mL	0.1429 mL	0.2858 mL	0.7144 mL

FDI-6 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FDI-6313380-27-7FDI6FDI 6DNA/RNA SynthesisInhibitorinhibitorinhibit

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FDI-6

Customer Review

FDI-6 Related Antibodies

2 Publications Citing Use of MCE FDI-6

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

FDI-6 Related Antibodies

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Complete Stock Solution Preparation Table

FDI-6 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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