Guanosine (Synonyms: DL-Guanosine; Vernine)

Name: Guanosine
Brand: MedChemExpress
SKU: HY-N0097
Rating: 5.0 (9 reviews)

Cat. No.: HY-N0097 Purity: 99.31%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Guanosine (DL-Guanosine) is a purine nucleoside comprising guanine attached to a ribose (ribofuranose) ring via a β-N9-glycosidic bond. Guanosine possesses anti-HSV activity.

For research use only. We do not sell to patients.

Guanosine Chemical Structure

CAS No. : 118-00-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	USD 30	In-stock	Estimated Time of Arrival: December 31
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Based on 9 publication(s) in Google Scholar

Other Forms of Guanosine:

7 Publications Citing Use of MCE Guanosine

•Front Cardiovasc Med. 2022 Nov 16;9:1042852. [Abstract]

: Guanosine purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2019 Oct;114:226-232. [Abstract]; The viral VP1 protein and β-actin are detected by western blot. β-actin was used as an internal control. A serial dilution of guanosine (from 100 μM to 25μM) are added to FMDV infected IBRS-2 cells, when treated with 25 μM merimepodib for 48 h. Cell viability and VP1 protein is analysed as described above.

: Guanosine purchased from MedChemExpress. Usage Cited in: Molecules. 2019 May 3;24(9):1723. [Abstract]; Guanosine supplementation attenuated the anti-FMDV effect of mizoribine. Serial dilutions of guanosine (from 100 to 25 μM) are added to FMDV (100 TCID₅₀ O/MY98/BY/2010)-infected IBRS-2 cells, when treated with 75 μM mizoribine for 48 h. (A) The cell viability of cells and (B) FMDV VP1 levels are analyzed.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Guanosine (DL-Guanosine) is a purine nucleoside comprising guanine attached to a ribose (ribofuranose) ring via a β-N9-glycosidic bond. Guanosine possesses anti-HSV activity.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Guanosine can be phosphorylated to become guanosine monophosphate (GMP), cyclic guanosine monophosphate (cGMP), guanosine diphosphate (GDP), and guanosine triphosphate (GTP). These forms play important roles in various biochemical processes such as synthesis of nucleic acids and proteins, photosynthesis, muscle contraction, and intracellular signal transduction (cGMP).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

283.24

Formula

C₁₀H₁₃N₅O₅

CAS No.

118-00-3

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=C(N)NC3=O)=C3N=C2)O1

Structure Classification

Others

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 75 mg/mL (264.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (3.53 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5306 mL	17.6529 mL	35.3057 mL
5 mM	0.7061 mL	3.5306 mL	7.0612 mL
10 mM	0.3531 mL	1.7653 mL	3.5306 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 3.33 mg/mL (11.76 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3.33 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.65%

Select Batch:

Data Sheet (275 KB) SDS (479 KB)

COA (251 KB) HNMR (121 KB) RP-HPLC (191 KB)

Handling Instructions (2659 KB)

References

[1]. De Clercq E1. Guanosine analogues as anti-herpesvirus agents. Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1531-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.5306 mL	17.6529 mL	35.3057 mL	88.2644 mL
DMSO	5 mM	0.7061 mL	3.5306 mL	7.0611 mL	17.6529 mL
	10 mM	0.3531 mL	1.7653 mL	3.5306 mL	8.8264 mL
	15 mM	0.2354 mL	1.1769 mL	2.3537 mL	5.8843 mL
	20 mM	0.1765 mL	0.8826 mL	1.7653 mL	4.4132 mL
	25 mM	0.1412 mL	0.7061 mL	1.4122 mL	3.5306 mL
	30 mM	0.1177 mL	0.5884 mL	1.1769 mL	2.9421 mL
	40 mM	0.0883 mL	0.4413 mL	0.8826 mL	2.2066 mL
	50 mM	0.0706 mL	0.3531 mL	0.7061 mL	1.7653 mL
	60 mM	0.0588 mL	0.2942 mL	0.5884 mL	1.4711 mL
	80 mM	0.0441 mL	0.2207 mL	0.4413 mL	1.1033 mL
	100 mM	0.0353 mL	0.1765 mL	0.3531 mL	0.8826 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Guanosine Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Guanosine118-00-3DL-Guanosine VernineHSVEndogenous MetaboliteHerpes simplex virusInhibitorinhibitorinhibit

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Guanosine (Synonyms: DL-Guanosine; Vernine)

Customer Review

Other Forms of Guanosine:

Guanosine Related Antibodies

7 Publications Citing Use of MCE Guanosine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HSV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Guanosine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Guanosine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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