Isobavachalcone (Synonyms: Corylifolinin; Isobacachalcone)

Name: Isobavachalcone
Brand: MedChemExpress
SKU: HY-13065
Rating: 5.0 (9 reviews)

Cat. No.: HY-13065 Purity: 99.01%: FAQs
Data Sheet SDS COA Handling Instructions

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Isobavachalcone (Corylifolinin) is derived from Psoralea corylifolia Linn. and is a potent inhibitor of Akt signaling pathway, which induces apoptosis in human cancer cells (Inhibits OVCAR-8 cell growth with an IC₅₀ value of 7.92 μM). Isobavachalcone also induces Reactive Oxyen Species (ROS) generation in OVCAR-8 cells and has exhibit cancer anti-promotive and anti-proliferative activity.

For research use only. We do not sell to patients.

Isobavachalcone Chemical Structure

CAS No. : 20784-50-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 77	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 77	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 115	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
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Based on 9 publication(s) in Google Scholar

Other Forms of Isobavachalcone:

Isobavachalcone (Standard) Get quote

8 Publications Citing Use of MCE Isobavachalcone

: Isobavachalcone purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2013 Jan;15(1):38-41. [Abstract]; IBC inhibits iNOS protein induced by TLRs agonists. A–C) RAW264.7 cells are pretreated with 20 or 50 μM IBC for 1 h and then further stimulated with MALP-2 (10 μg/mL) (A), LPS (10 μg/mL) (B), or poly[I:C] (10 μg/mL) (C) for an additional 8 h. Cell lysates are analyzed for iNOS and β-actin protein by immunoblots. Veh, vehicle; IBC, isobavachalcone.

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC50: 7.92 μM (OVCAR-8 cell)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: 8	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 25710081]
HUVEC	IC₅₀	> 100 μM Compound: 1c	Toxicity against HUVEC incubated for 48 hrs by MTT assay Toxicity against HUVEC incubated for 48 hrs by MTT assay	[PMID: 25590864]
K562	IC₅₀	> 10 μM Compound: 8	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 25710081]
K562	IC₅₀	29.49 μM Compound: 1c	Antitumor activity against human K562 cells incubated for 48 hrs by MTT assay Antitumor activity against human K562 cells incubated for 48 hrs by MTT assay	[PMID: 25590864]
OVCAR-8	IC₅₀	7.92 μM Compound: 60; IBC	Cytotoxicity against human OVCAR-8 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human OVCAR-8 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35151222]
PC-3	IC₅₀	15.06 μM Compound: 60; IBC	Cytotoxicity against human PC3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35151222]
Platelet	IC₅₀	0.5 μM Compound: 1	Antiplatelet activity against rabbit platelet assessed as inhibition of arachidonic acid-induced platelet aggregation preincubated for 3 mins by turbidimetric method Antiplatelet activity against rabbit platelet assessed as inhibition of arachidonic acid-induced platelet aggregation preincubated for 3 mins by turbidimetric method	[PMID: 8759164]
Platelet	IC₅₀	41.6 μM Compound: 1	Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation preincubated for 3 mins by turbidimetric method Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation preincubated for 3 mins by turbidimetric method	[PMID: 8759164]
Platelet	IC₅₀	65.1 μM Compound: 1	Antiplatelet activity against rabbit platelet assessed as inhibition of collagen-induced platelet aggregation preincubated for 3 mins by turbidimetric method Antiplatelet activity against rabbit platelet assessed as inhibition of collagen-induced platelet aggregation preincubated for 3 mins by turbidimetric method	[PMID: 8759164]
RAW264.7	IC₅₀	16.4 μM Compound: 2	Cytotoxicity against mouse RAW264.7 cells by MTT assay Cytotoxicity against mouse RAW264.7 cells by MTT assay	[PMID: 16643064]
RAW264.7	IC₅₀	6.4 μM Compound: 2	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs	[PMID: 16643064]
THP-1	CC₅₀	35.95 μM Compound: 1	Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 26906638]
Vero	IC₅₀	31.25 μg/mL Compound: 14, 4-hydroxy-isocordoin	Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 72 hrs by MTT assay	[PMID: 22169259]
Vero	CC₅₀	726.82 μg/mL Compound: 14, 4-hydroxy-isocordoin	Cytotoxicity against african green monkey Vero cells assessed as cell death after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells assessed as cell death after 72 hrs by MTT assay	[PMID: 22169259]

In Vitro

Isobavachalcone (6.0-48.0 μM; 72 hours; OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells) treatment inhibits the proliferation of human cancer cells. Isobavachalcone inhibits PC3, A549, MCF-7, L-02 and HUVEC cells growth with IC₅₀ values of 15.06 μM, 32.2 μM, 28.29 μM, 31.61 μM and 31.3 μM, respectively^[1].
Isobavachalcone (0-18 μM; 6 hours; OVCAR-8 and PC3 cells) treatment results in a concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt and GSK3b phosphorylation^[1].
Isobavachalcone (0-18 μM; 72 hours; OVCAR-8 and PC3 cells) treatment causes apoptosis via caspase- and ROS-involved mitochondrial pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells
Concentration:	6.0-48.0 μM
Incubation Time:	72 hours
Result:	Inhibited the proliferation of human cancer cells.

Western Blot Analysis^[1]

Cell Line:	OVCAR-8 or PC3 cells
Concentration:	0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:	6 hours
Result:	A concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt. GSK3b phosphorylation was also inhibited in a concentration- and time-dependent manner.

Apoptosis Analysis^[1]

Cell Line:	OVCAR-8 cells and PC3 cells
Concentration:	0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:	72 hours
Result:	Led to dose dependent increase of apoptosis.

In Vivo

Isobavachalcone (20 mg/kg; intraperitoneal injection; for 0.5-24 hours; female Kunming mice) treatment results in an increase in blood glucose levels, reaching a maximum within 1 hour and maintaining until 4 hours post-dosing^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Seven-week-old specific pathogen-free female Kunming mice (18-22 g)^[1]
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; for 0.5, 1, 2, 4, 6, 8, 12, 24 hours
Result:	Increased in blood glucose levels.

Molecular Weight

324.37

Formula

C₂₀H₂₀O₄

CAS No.

20784-50-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(/C=C/C1=CC=C(C=C1)O)C2=CC=C(C(C/C=C(C)\C)=C2O)O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 11 mg/mL (33.91 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0829 mL	15.4145 mL	30.8290 mL
5 mM	0.6166 mL	3.0829 mL	6.1658 mL
10 mM	0.3083 mL	1.5414 mL	3.0829 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.01%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (244 KB) LCMS (144 KB)

Handling Instructions (2659 KB)

References

[1]. Jing H, et al. Abrogation of Akt signaling by Isobavachalcone contributes to its anti-proliferative effects towards human cancer cells. Cancer Lett. 2010 Aug 28;294(2):167-77. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0829 mL	15.4145 mL	30.8290 mL	77.0725 mL
	5 mM	0.6166 mL	3.0829 mL	6.1658 mL	15.4145 mL
	10 mM	0.3083 mL	1.5414 mL	3.0829 mL	7.7072 mL
	15 mM	0.2055 mL	1.0276 mL	2.0553 mL	5.1382 mL
	20 mM	0.1541 mL	0.7707 mL	1.5414 mL	3.8536 mL
	25 mM	0.1233 mL	0.6166 mL	1.2332 mL	3.0829 mL
	30 mM	0.1028 mL	0.5138 mL	1.0276 mL	2.5691 mL

Isobavachalcone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isobavachalcone20784-50-3Corylifolinin IsobacachalconeAktReactive Oxygen SpeciesApoptosisAutophagyPKBProtein kinase BInhibitorinhibitorinhibit

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Isobavachalcone (Synonyms: Corylifolinin; Isobacachalcone)

Customer Review

Other Forms of Isobavachalcone:

Isobavachalcone Related Antibodies

8 Publications Citing Use of MCE Isobavachalcone

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•Related Screening Libraries:

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View All Akt Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Isobavachalcone Related Antibodies

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Complete Stock Solution Preparation Table

Isobavachalcone Related Classifications

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