JH-X-119-01 hydrochloride

Name: JH-X-119-01 hydrochloride
Brand: MedChemExpress
SKU: HY-103017
Rating: 5.0 (3 reviews)

Cat. No.: HY-103017 Purity: 98.15%: FAQs
Data Sheet SDS COA Handling Instructions

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JH-X-119-01 hydrochloride is a potent and selective interleukin-1 receptor-associated kinases 1 (IRAK1) inhibitor. JH-X-119-01 hydrochloride ameliorates LPS-induced sepsis in mice.

For research use only. We do not sell to patients.

JH-X-119-01 hydrochloride Chemical Structure

CAS No. : 2591344-30-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 559	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 559	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3300	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of JH-X-119-01 hydrochloride:

JH-X-119-01 Get quote

3 Publications Citing Use of MCE JH-X-119-01 hydrochloride

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IRAK1 IRAK4

Biological Activity
Purity & Documentation
References
Customer Review

Description

JH-X-119-01 hydrochloride is a potent and selective interleukin-1 receptor-associated kinases 1 (IRAK1) inhibitor. JH-X-119-01 hydrochloride ameliorates LPS-induced sepsis in mice^[1].

IC₅₀ & Target^[1]

IRAK1

In Vitro

Jh-X-119-01 (10 μM) decreases phosphorylation of NF-κB and mRNA levels of IL-6 and TNFα in LPS-treated macrophages in vitro. Jh-X-119-01 selectively inhibits IRAK1 phosphorylation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	RAW 264.7 cells and THP-1 cells
Concentration:	10 μM
Incubation Time:	15 minutes
Result:	Decreased LPS (100 ng/mL)-induced phosphorylation of IκBα and NF-κB-P65.

In Vivo

Jh-X-119-01 improves survival and decreases immunopathies of LPS-challenged mice. Jh-X-119-01 increases survival of mice at the dose of 5 mg/kg body weight. Survival is further improved when the dose is increased to 10 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 (20-22 g, male) mice^[1]
Dosage:	5 mg/kg and 10 mg/kg
Administration:	Intraperitoneally injected; 5 days
Result:	Protected mice from LPS (20 mg/kg)-induced sepsis. Survival at day 5 was 13.3% in control group where septic mice were treated by vehicle, while the values were 37.5% and 56.3% for 5 mg/kg and 10 mg/kg.

Molecular Weight

488.93

Formula

C₂₅H₂₁ClN₆O₃

CAS No.

2591344-30-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C=C)NC1=CC=CC(C(NC2=CC=C(NC(C3=NC(C4=CC=NN4)=CC=C3)=O)C=C2)=O)=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (102.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0453 mL	10.2264 mL	20.4528 mL
5 mM	0.4091 mL	2.0453 mL	4.0906 mL
10 mM	0.2045 mL	1.0226 mL	2.0453 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.11 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.15%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (250 KB) HNMR (124 KB) RP-HPLC (161 KB) LCMS (417 KB)

Handling Instructions (2659 KB)

References

[1]. Bin Pan, et al. Selective inhibition of interleukin-1 receptor-associated kinase 1 ameliorates lipopolysaccharide-induced sepsis in mice. Int Immunopharmacol. 2020 Aug;85:106597. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0453 mL	10.2264 mL	20.4528 mL	51.1321 mL
	5 mM	0.4091 mL	2.0453 mL	4.0906 mL	10.2264 mL
	10 mM	0.2045 mL	1.0226 mL	2.0453 mL	5.1132 mL
	15 mM	0.1364 mL	0.6818 mL	1.3635 mL	3.4088 mL
	20 mM	0.1023 mL	0.5113 mL	1.0226 mL	2.5566 mL
	25 mM	0.0818 mL	0.4091 mL	0.8181 mL	2.0453 mL
	30 mM	0.0682 mL	0.3409 mL	0.6818 mL	1.7044 mL
	40 mM	0.0511 mL	0.2557 mL	0.5113 mL	1.2783 mL
	50 mM	0.0409 mL	0.2045 mL	0.4091 mL	1.0226 mL
	60 mM	0.0341 mL	0.1704 mL	0.3409 mL	0.8522 mL
	80 mM	0.0256 mL	0.1278 mL	0.2557 mL	0.6392 mL
	100 mM	0.0205 mL	0.1023 mL	0.2045 mL	0.5113 mL