L-SelenoMethionine

Name: L-SelenoMethionine
Brand: MedChemExpress
SKU: HY-B1000A
Rating: 5.0 (2 reviews)

Cat. No.: HY-B1000A Purity: 99.82%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

L-SelenoMethionine, an L-isomer of Selenomethionine, is a major natural food-form of selenium. L-SelenoMethionin is a cancer chemopreventive agent that can reduce cancer incidence by dietary supplementation and induce apoptosis of cancer cells. L-SelenoMethionine also can increase expression of glutathione peroxidase.

For research use only. We do not sell to patients.

L-SelenoMethionine Chemical Structure

CAS No. : 3211-76-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Based on 2 publication(s) in Google Scholar

Other Forms of L-SelenoMethionine:

Selenomethionine In-stock
L-SelenoMethionine (Standard) Get quote

2 Publications Citing Use of MCE L-SelenoMethionine

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Microbial Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	65 μM Compound: 3	Anticancer activity against human A549 cells assessed as cell growth inhibition measured after 6 days by SRB assay Anticancer activity against human A549 cells assessed as cell growth inhibition measured after 6 days by SRB assay	[PMID: 34217061]
DU-145	IC₅₀	40 μM Compound: 3	Anticancer activity against human DU-145 cells assessed as cell growth inhibition measured after 6 days by SRB assay Anticancer activity against human DU-145 cells assessed as cell growth inhibition measured after 6 days by SRB assay	[PMID: 34217061]
HT-29	IC₅₀	130 μM Compound: 3	Anticancer activity against human HT-29 cells assessed as cell growth inhibition measured after 6 days by SRB assay Anticancer activity against human HT-29 cells assessed as cell growth inhibition measured after 6 days by SRB assay	[PMID: 34217061]
UACC-375	IC₅₀	50 μM Compound: 3	Anticancer activity against human UACC-375 cells assessed as cell growth inhibition measured after 6 days by SRB assay Anticancer activity against human UACC-375 cells assessed as cell growth inhibition measured after 6 days by SRB assay	[PMID: 34217061]

In Vitro

L-SelenoMethionine (1-500 μM; 24-72 h) is selectively inhibits the growth of prostate cancer cells, compared with normal cells^[2].
? L-SelenoMethionine (500 μM; 48 h) induce apoptosis in prostate cancer cells^[2].
? L-SelenoMethionine (500 μM; 48 h) causes an increase in arrest in the G2-M phase of the cell cycle selectively in prostate cancer cells^[2].
? L-SelenoMethionine (5 μM; 24 h) mitigates gene expression associated with the cellular stress response from 10 cGy irradiation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	1, 5, 10, 50, 100, 500 μM
Incubation Time:	24, 48, 72 hours
Result:	Inhibited the growth of prostate cancer cells, with a lower IC₅₀ (1-90 μM) at 72 h than normal prostate cells (>500 μM).

Apoptosis Analysis^[2]

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	500 μM
Incubation Time:	48 hours
Result:	Exhibited the highest level of DNA condensation in androgen-responsive LNCaP carcinoma cells, followed by PC-3 and DU145 cells. Exhibited the nicked-end DNA labeling in prostate cancer cells. Promoted the PARP cleavage in prostate cancer cells.

Cell Cycle Analysis^[2]

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	500 μM
Incubation Time:	48 hours
Result:	Increased the sub-G0-G1 cell fraction of LNCaP (41.5%), PC-3 (12.1%), and DU145 cells (11.2%). Caused a significant increases in G2 cells , particularly in LNCaP (13%), PC-3 (32%), and DU145 (20%) cells.

RT-PCR^[3]

Cell Line:	Human thyroid epithelial cells (HTori-3)
Concentration:	5 μM
Incubation Time:	24 hours
Result:	Downregulated the CDC6, GADD45A, FAS and ATF3 in irradiated cells.

In Vivo

L-SelenoMethionine (0.06-12 μg/g diet; p.o. for 3 d) partially or completely prevent the decrease in the serum or plasma levels of total antioxidants in rats exposed to gamma rays, protons or HZE particles^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

196.11

Formula

C₅H₁₁NO₂Se

CAS No.

3211-76-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@@H](N)CC[Se]C

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Animals

Microorganisms

Endogenous metabolite

Widespread

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 14.29 mg/mL (72.87 mM; Need ultrasonic)

DMSO : 1 mg/mL (5.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0992 mL	25.4959 mL	50.9918 mL
5 mM	1.0198 mL	5.0992 mL	10.1984 mL
10 mM	0.5099 mL	2.5496 mL	5.0992 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 9.09 mg/mL (46.35 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (277 KB) SDS (788 KB)

COA (245 KB) HNMR (241 KB) RP-HPLC (244 KB)

Handling Instructions (2659 KB)

References

[1]. Schrauzer GN, et, al. Selenomethionine: a review of its nutritional significance, metabolism and toxicity. J Nutr. 2000 Jul;130(7):1653-6. [Content Brief]

[2]. Menter DG, et, al. Selenium effects on prostate cell growth. Cancer Epidemiol Biomarkers Prev. 2000 Nov;9(11):1171-82. [Content Brief]

[3]. Jornot L, et, al. Differential regulation of glutathione peroxidase by selenomethionine and hyperoxia in endothelial cells. Biochem J. 1995 Mar 1;306 ( Pt 2)(Pt 2):581-7. [Content Brief]

[4]. Nuth M, et, al. Mitigating effects of L-selenomethionine on low-dose iron ion radiation-induced changes in gene expression associated with cellular stress. Oncol Lett. 2013 Jul;6(1):35-42. [Content Brief]

[5]. Guan J, et, al. Effects of dietary supplements on space radiation-induced oxidative stress in Sprague-Dawley rats. Radiat Res. 2004 Nov;162(5):572-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	5.0992 mL	25.4959 mL	50.9918 mL	127.4795 mL
DMSO / H₂O	5 mM	1.0198 mL	5.0992 mL	10.1984 mL	25.4959 mL
H₂O	10 mM	0.5099 mL	2.5496 mL	5.0992 mL	12.7479 mL
	15 mM	0.3399 mL	1.6997 mL	3.3995 mL	8.4986 mL
	20 mM	0.2550 mL	1.2748 mL	2.5496 mL	6.3740 mL
	25 mM	0.2040 mL	1.0198 mL	2.0397 mL	5.0992 mL
	30 mM	0.1700 mL	0.8499 mL	1.6997 mL	4.2493 mL
	40 mM	0.1275 mL	0.6374 mL	1.2748 mL	3.1870 mL
	50 mM	0.1020 mL	0.5099 mL	1.0198 mL	2.5496 mL
	60 mM	0.0850 mL	0.4249 mL	0.8499 mL	2.1247 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.