2. Apoptosis
  3. Caspase
  4. ML132

ML132  (Synonyms: NCGC-00183434)

Cat. No.: HY-12412 Purity: 98.05%
COA Handling Instructions

Molarity Calculator

ML132 (NCGC-00183434) is a selective caspase 1 inhibitor with an IC50 of 34.9 nM. ML132 shows good stability that can be utilized as molecular probes of caspase 1. ML132 can be used for research in the field of anti-cancer and anti-inflammatory.

For research use only. We do not sell to patients.

ML132 Chemical Structure

ML132 Chemical Structure

CAS No. : 1230628-71-3

Size Price Stock Quantity
1 mg USD 160 In-stock
5 mg USD 400 In-stock
10 mg USD 660 In-stock
50 mg USD 1980 In-stock
100 mg USD 3150 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

ML132 Related Antibodies

Top Publications Citing Use of Products

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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  • Purity & Documentation

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  • Customer Review

Description

ML132 (NCGC-00183434) is a selective caspase 1 inhibitor with an IC50 of 34.9 nM. ML132 shows good stability that can be utilized as molecular probes of caspase 1. ML132 can be used for research in the field of anti-cancer and anti-inflammatory[1].

IC50 & Target[1]

Caspase-1

34.9 nM (IC50)

Caspase-4

1.27 μM (IC50)

Caspase-5

0.85 μM (IC50)

Caspase-8

4.18 μM (IC50)

Caspase-9

2.85 μM (IC50)

Molecular Weight

477.94

Formula

C22H28ClN5O5
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

ClC1=CC(C(N[C@@H](C(C)(C)C)C(N2CCC[C@H]2C(N[C@@H](CC(O)=O)C#N)=O)=O)=O)=CC=C1N
Shipping

Shipping with dry ice.
Storage

-80°C, protect from light, stored under nitrogen
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (523.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0923 mL 10.4616 mL 20.9231 mL
5 mM 0.4185 mL 2.0923 mL 4.1846 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 7 mg/mL (14.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 7 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (70.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 6.25 mg/mL (13.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.75%

COA (253 KB) HNMR (582 KB) LCMS (109 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0923 mL 10.4616 mL 20.9231 mL 52.3078 mL
5 mM 0.4185 mL 2.0923 mL 4.1846 mL 10.4616 mL
10 mM 0.2092 mL 1.0462 mL 2.0923 mL 5.2308 mL
15 mM 0.1395 mL 0.6974 mL 1.3949 mL 3.4872 mL
20 mM 0.1046 mL 0.5231 mL 1.0462 mL 2.6154 mL
25 mM 0.0837 mL 0.4185 mL 0.8369 mL 2.0923 mL
30 mM 0.0697 mL 0.3487 mL 0.6974 mL 1.7436 mL
40 mM 0.0523 mL 0.2615 mL 0.5231 mL 1.3077 mL
50 mM 0.0418 mL 0.2092 mL 0.4185 mL 1.0462 mL
60 mM 0.0349 mL 0.1744 mL 0.3487 mL 0.8718 mL
80 mM 0.0262 mL 0.1308 mL 0.2615 mL 0.6538 mL
100 mM 0.0209 mL 0.1046 mL 0.2092 mL 0.5231 mL
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ML132 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML1321230628-71-3NCGC-00183434ML 132ML-132NCGC00183434NCGC 00183434Caspasecysteine proteasescancerinflammationInhibitorinhibitorinhibit

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