2. TGF-beta/Smad
  3. TGF-β Receptor
  4. ML347

ML347 (LDN193719) is a highly selective ALK1/ALK2 inhibitor. ML347 has IC50 values of 46 and 32 nM against ALK1 and ALK2, respectively, >300-fold selective over ALK3. ML347 block the phosphorylation of Smad1/5 by TGF-β1.

For research use only. We do not sell to patients.

ML347 Chemical Structure

ML347 Chemical Structure

CAS No. : 1062368-49-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid
1 mg USD 26 In-stock
5 mg USD 66 In-stock
10 mg USD 99 In-stock
50 mg USD 385 In-stock
100 mg USD 660 In-stock
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500 mg   Get quote  

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Customer Review

Based on 7 publication(s) in Google Scholar

ML347 Related Antibodies

Top Publications Citing Use of Products

    ML347 purchased from MedChemExpress. Usage Cited in: Patent. US20170369886A1.

    FIG illustrates angiogenesis in the absence and presence of an Alk1 small molecule inhibitor (ML347) in presence or absence of inhibin α.

    View All TGF-β Receptor Isoform Specific Products:

    View All Isoforms
    ALK1 ALK2 ALK3 ALK4 ALK5 ALK6 ALK7 TGFβR2 ACVR2A ACVR2B

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ML347 (LDN193719) is a highly selective ALK1/ALK2 inhibitor. ML347 has IC50 values of 46 and 32 nM against ALK1 and ALK2, respectively, >300-fold selective over ALK3. ML347 block the phosphorylation of Smad1/5 by TGF-β1[1][2].

    IC50 & Target[1]

    ALK1

    46 nM (IC50)

    ACVR1

    32 nM (IC50)

    BMPR1A

    10800 nM (IC50)

    In Vitro

    ML347 can inhibit ALK1/ALK2 to block TGF?β signal transduction[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ML347 Related Antibodies

    Immunofluorescence[2]

    Cell Line: Primary dental epithelial cells were cultured in Dulbecco’s Modifed Eagle Medium (DMEM)/ F12 supplemented with 20% fetal bovine serum and 1% penicillin/streptomycin.
    Concentration: 25 μM
    Incubation Time: 2 hours
    Result: Inhibited ALK1/ALK2 then blocking Smad1/5 by TGF-β1.
    Molecular Weight

    352.39

    Formula

    C22H16N4O
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    COC1=CC=C(C2=CN3C(N=C2)=C(C4=C5C=CC=NC5=CC=C4)C=N3)C=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (28.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8378 mL 14.1888 mL 28.3776 mL
    5 mM 0.5676 mL 2.8378 mL 5.6755 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.94%

    COA (256 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.8378 mL 14.1888 mL 28.3776 mL 70.9441 mL
    5 mM 0.5676 mL 2.8378 mL 5.6755 mL 14.1888 mL
    10 mM 0.2838 mL 1.4189 mL 2.8378 mL 7.0944 mL
    15 mM 0.1892 mL 0.9459 mL 1.8918 mL 4.7296 mL
    20 mM 0.1419 mL 0.7094 mL 1.4189 mL 3.5472 mL
    25 mM 0.1135 mL 0.5676 mL 1.1351 mL 2.8378 mL
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    ML347 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ML3471062368-49-3LDN 193719ML 347ML-347LDN193719LDN-193719TGF-β ReceptorTransforming growth factor beta receptorsALK1；ALK2； Smad1/5Inhibitorinhibitorinhibit

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