2. Cytoskeleton Autophagy Apoptosis
  3. Dynamin Mitophagy Autophagy Apoptosis
  4. Mdivi-1

Mdivi-1  (Synonyms: Mitochondrial division inhibitor 1)

Cat. No.: HY-15886 Purity: 99.96%
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Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor. Mdivi-1 is a mitochondrial division/mitophagy inhibitor.

For research use only. We do not sell to patients.

Mdivi-1 Chemical Structure

Mdivi-1 Chemical Structure

CAS No. : 338967-87-6

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Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 65 In-stock
50 mg USD 190 In-stock
100 mg USD 290 In-stock
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Customer Review

Based on 168 publication(s) in Google Scholar

Mdivi-1 Related Antibodies

Top Publications Citing Use of Products

159 Publications Citing Use of MCE Mdivi-1

WB
IHC

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2023 Feb 16;280:109697.

    S.aureus-induced reduction of mitochondrial proteins is blocked by Mdivi-1 (20 μM; 2 h) in BMECs.

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: Theranostics. 2019 Feb 28;9(6):1698-1713.  [Abstract]

    Western blotting of SDs and markers of podocyte dedifferentiation with the treatment of PA , BBR + PA and Mdivi1+PA.

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232.  [Abstract]

    HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 24 h. Mitochondrial colocalization with LC3 is detected by immunofluorescence.

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232.  [Abstract]

    HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 48 h. Apoptosis-related proteins expression level is detected by western blotting. β-actin was used as a loading control.

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: Brain Res. 2019 May 15;1711:68-76.  [Abstract]

    Levels of CytC in cytosol (Cyto-CytC), CytC in mitochondria (Mito- CytC) and cleaved caspase-3 in hippocampus are determined by western blot with the treatment of sham, HIR, MDV or FK506.

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: J Pineal Res. 2018 Sep;65(2):e12491.  [Abstract]

    Inhibition of mitochondrial fission with mdivi-1 or Drp1 siRNA suppresses hyperglycemia-induced oxidative stress. Protein expression of Nox4.

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: Transl Res. 2018 Apr;194:68-78.  [Abstract]

    Representative western blot analysis of pDrp1-616 and total Drp1 protein expression in the hippocampus (left). Tubulin is used as the loading control. Protein expressions are quantified by western blot band intensity (right).

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2017 Nov;112:336-349.  [Abstract]

    Mdivi-1 administration reduces NF-κB-dependent inflammation. A. Western blot assay and quantitative analysis of Cyto-p65 (A) and Nec-p65 (B) expression. ß-actin and H3 are used as internal controls of cytosolic and nuclear subfractions, respectively.

    Mdivi-1 purchased from MedChemExpress. Usage Cited in: Exp Neurol. 2017 Sep;295:116-124.  [Abstract]

    Mdivi-1 treatment inhibits the expression of DRP1, alleviates ROS and reduces the expression of NLRP3 and cleaved caspase-1 at 24 h after SAH. Representative Western blots showing levels of DRP1.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor. Mdivi-1 is a mitochondrial division/mitophagy inhibitor.

    In Vitro

    Mdivi-1 inhibits Dnm1 GTPase activity in a dose-dependent manner, with an estimated EC50 of 1-10 μM. Mdivi-1 increases the apparent K0.5 for GTP, lowers the apparent Vmax for GTP hydrolysis, and causes an increase in the Hill coefficient observed for GTP in the Dnm1 GTP hydrolysis reaction[1]. Cells treated with mdivi-1 display decreased cytochrome c release and a reduced rate of phosphatidylserine exposure on their surface following apoptosis induction, consistent with an inhibition of apoptosis and with previous studies using other strategies to compromise DRP1 activity[2]. Mdivi-1 results in apoptotic cell death in ischemic retina[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Mdivi-1 Related Antibodies
    In Vivo

    The mitochondrial division DRP, Dnm1, is the target of mdivi-1 in vivo. Mdivi-1 quantitatively blocks GMPPCP- dependent Dnm1 self-assembly in a concentration range similar to its effects on mitochondrial division in vivo[1]. Mdivi-1 (50 mg/kg, i.p.) significantly decreases GFAP protein expression in the normal mouse retina[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    353.22

    Formula

    C15H10Cl2N2O2S
    CAS No.
    Appearance

    Solid

    Color

    White to light brown

    SMILES

    O=C1N(C2=C(Cl)C=C(Cl)C(OC)=C2)C(S)=NC3=C1C=CC=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 110 mg/mL (311.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8311 mL 14.1555 mL 28.3110 mL
    5 mM 0.5662 mL 2.8311 mL 5.6622 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 4 mg/mL (11.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 4 mg/mL (11.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 2.5 mg/mL (7.08 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  17% Solutol HS-15 in Saline

      Solubility: 10 mg/mL (28.31 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.96%

    COA (253 KB) HNMR (320 KB) RP-HPLC (265 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [1]

    YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.8311 mL 14.1555 mL 28.3110 mL 70.7774 mL
    5 mM 0.5662 mL 2.8311 mL 5.6622 mL 14.1555 mL
    10 mM 0.2831 mL 1.4155 mL 2.8311 mL 7.0777 mL
    15 mM 0.1887 mL 0.9437 mL 1.8874 mL 4.7185 mL
    20 mM 0.1416 mL 0.7078 mL 1.4155 mL 3.5389 mL
    25 mM 0.1132 mL 0.5662 mL 1.1324 mL 2.8311 mL
    30 mM 0.0944 mL 0.4718 mL 0.9437 mL 2.3592 mL
    40 mM 0.0708 mL 0.3539 mL 0.7078 mL 1.7694 mL
    50 mM 0.0566 mL 0.2831 mL 0.5662 mL 1.4155 mL
    60 mM 0.0472 mL 0.2359 mL 0.4718 mL 1.1796 mL
    80 mM 0.0354 mL 0.1769 mL 0.3539 mL 0.8847 mL
    100 mM 0.0283 mL 0.1416 mL 0.2831 mL 0.7078 mL
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    Mdivi-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Mdivi-1338967-87-6Mitochondrial division inhibitor 1Mdivi1Mdivi 1Mitochondrial division inhibitor1Mitochondrial division inhibitor-1DynaminMitophagyAutophagyApoptosisMitochondrial AutophagyInhibitorinhibitorinhibit

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