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Menadione bisulfite sodium  (Synonyms: Menadione sodium bisulfite; Vitamin K3 sodium bisulfite)

Cat. No.: HY-B1897A Purity: 99.87%
COA Handling Instructions

Molarity Calculator

Menadione bisulfite (sodium) is used as an agent to induce acute oxidative stress, and to function as a plant-defense activator against several pathogens.

For research use only. We do not sell to patients.

Menadione bisulfite sodium Chemical Structure

Menadione bisulfite sodium Chemical Structure

CAS No. : 130-37-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Solid
500 mg USD 30 In-stock
1 g USD 45 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Menadione bisulfite sodium:

Menadione bisulfite sodium Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Menadione bisulfite (sodium) is used as an agent to induce acute oxidative stress, and to function as a plant-defense activator against several pathogens.

In Vitro

Menadione sodium bisulfite (500 μM) treatment and the concomitant ROS increase have an important impact on the photosynthetic activity of the cells. Global and specific antioxidant defences decrease with Menadione sodium bisulfite (500 μM) treatment[1]. Menadione sodium bisulfite and PABA activate similar defense responses (PR, ROS, and HR). Menadione sodium bisulfite enhances resistance, both locally and systemically, to phoma stem canker disease caused by Leptosphaeria maculans. Menadione sodium bisulfite treatment induces resistance against downy mildew in pearl millet. Menadione sodium bisulfite acts through priming of specific sets of the plant innate-defense repertoire. Menadione sodium bisulfite treatment up-regulates the expression of a gene encoding a GRX480 protein, a member of the glutaredoxin family that regulates protein redox state[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Menadione bisulfite sodium Related Antibodies
Molecular Weight

276.24

Formula

C11H9NaO5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(C(CC(C1=C2C=CC=C1)=O)(C)C2=O)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (362.00 mM)

DMSO : 100 mg/mL (362.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6200 mL 18.1002 mL 36.2004 mL
5 mM 0.7240 mL 3.6200 mL 7.2401 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 120 mg/mL (434.40 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.87%

COA (253 KB) LCMS (286 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

Briefly, cells are lysed by sonication (6 cycles of 10 s separated by 30 s, on ice) in a buffer containing 25 mM Hepes at pH 7.5, 5 mM MgCl2, 5 mM DTT, 2 mM PMSF, 5 mM EDTA, 400 mM sorbitol and 10 μg/mL Protease Inhibitor cocktail. A quantity of 40 μg Symbiodinium protein extract is incubated in the reaction buffer. The mixture consists of 50 μM Ac-DEVD-AFC, 100 mM Hepes at pH 7.5, 10% (v/v) sucrose, 0.1% (v/v) CHAPS, 10 mM DTT and 10 mM PMSF. Samples are then incubated in black 96-well microplates and the fluorescence emitted is measured every 3 min for 90 min at 505 nm, with a 400 nm excitation wavelength provided by a FLX-Xenius spectrofluorometer. Caspase-like activities are then expressed in pmol of AFC cleavage per minute and illustrated as percentage of the controls.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.6200 mL 18.1002 mL 36.2004 mL 90.5010 mL
5 mM 0.7240 mL 3.6200 mL 7.2401 mL 18.1002 mL
10 mM 0.3620 mL 1.8100 mL 3.6200 mL 9.0501 mL
15 mM 0.2413 mL 1.2067 mL 2.4134 mL 6.0334 mL
20 mM 0.1810 mL 0.9050 mL 1.8100 mL 4.5251 mL
25 mM 0.1448 mL 0.7240 mL 1.4480 mL 3.6200 mL
30 mM 0.1207 mL 0.6033 mL 1.2067 mL 3.0167 mL
40 mM 0.0905 mL 0.4525 mL 0.9050 mL 2.2625 mL
50 mM 0.0724 mL 0.3620 mL 0.7240 mL 1.8100 mL
60 mM 0.0603 mL 0.3017 mL 0.6033 mL 1.5084 mL
80 mM 0.0453 mL 0.2263 mL 0.4525 mL 1.1313 mL
100 mM 0.0362 mL 0.1810 mL 0.3620 mL 0.9050 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Menadione bisulfite sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Menadione bisulfite130-37-0Menadione sodium bisulfite Vitamin K3 sodium bisulfiteOthersInhibitorinhibitorinhibit

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