2. Autophagy Metabolic Enzyme/Protease Apoptosis
  3. Autophagy Drug Metabolite Apoptosis
  4. Nortriptyline

Nortriptyline  (Synonyms: Desmethylamitriptyline; Desitriptilina)

Cat. No.: HY-118620 Purity: 99.97%
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Nortriptyline (Desmethylamitriptyline), the main active metabolite of Amitriptyline, is a tricyclic antidepressant. Nortriptyline is a potent autophagy inhibitor and has anticancer effects. N

For research use only. We do not sell to patients.

Nortriptyline Chemical Structure

Nortriptyline Chemical Structure

CAS No. : 72-69-5

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Based on 2 publication(s) in Google Scholar

Other Forms of Nortriptyline:

Nortriptyline Related Antibodies

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Description

Nortriptyline (Desmethylamitriptyline), the main active metabolite of Amitriptyline, is a tricyclic antidepressant. Nortriptyline is a potent autophagy inhibitor and has anticancer effects[1][2][3]. N

In Vitro

Amitriptyline is metabolized by CYP2C19 to the active metabolite, Nortriptyline. Nortriptyline blocks the reuptake of Norepinephrine more potently than Serotonin[1].
Nortriptyline (6.25-100 μM; 24-72 h) markedly reducs the viability of TCCSUP and mouse MBT-2 bladder cancer cells in a concentration- and time-dependent manner[3].
Nortriptyline (12.55-100 μM; 24 h) induces cell cycle arrest and apoptosis in TCCSUP and MBT-2 cells[3].
Nortriptyline (12.55-100 μM; 24 h) induces both intrinsic and extrinsic apoptosis in these bladder cancer cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nortriptyline Related Antibodies

Cell Viability Assay[3]

Cell Line: Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration: 6.25 μM, 12.5 μM, 25 μM, 50 μM and 100 μM
Incubation Time: 24, 48, or 72 h
Result: Exhibited cytotoxic effects on TCCSUP and MBT-2 cells.

Cell Cycle Analysis[3]

Cell Line: Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration: 25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time: 24 h
Result: Caused cell cycle arrest in these bladder cancer cells.

Apoptosis Analysis[3]

Cell Line: Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration: 25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time: 24 h
Result: Induced apoptosis in both TCCSUP and MBT-2 cells.

Western Blot Analysis[3]

Cell Line: Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration: 25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time: 24 h
Result: Increased the expression of Fas, FasL, FADD, Bax, Bak, and cleaved forms of caspase-3, caspase-8, caspase-9, and poly(ADP-ribose) polymerase. Decreased the expression of Bcl-2, Bcl-xL, BH3 interacting domain death agonist, X-linked inhibitor of apoptosis protein, and survivin.
In Vivo

Nortriptyline (10-20 mg/kg; ip; every day; for three weeks) inhibits the growth of bladder tumors in mice inoculated with MBT-2 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male C3H/HeN mice (25-30 g; 2-3 months of age) injected with MBT-2 cells[3]
Dosage: 10 or 20 mg/kg
Administration: Intraperitoneal injection; every day; for three weeks.
Result: Suppressed tumor growth in mice inoculated with MBT-2 cells.
Clinical Trial
Molecular Weight

263.38

Formula

C19H21N
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CNCC/C=C1C2=CC=CC=C2CCC3=CC=CC=C\13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (379.68 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7968 mL 18.9840 mL 37.9680 mL
5 mM 0.7594 mL 3.7968 mL 7.5936 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.97%

COA (255 KB) LCMS (92 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7968 mL 18.9840 mL 37.9680 mL 94.9199 mL
5 mM 0.7594 mL 3.7968 mL 7.5936 mL 18.9840 mL
10 mM 0.3797 mL 1.8984 mL 3.7968 mL 9.4920 mL
15 mM 0.2531 mL 1.2656 mL 2.5312 mL 6.3280 mL
20 mM 0.1898 mL 0.9492 mL 1.8984 mL 4.7460 mL
25 mM 0.1519 mL 0.7594 mL 1.5187 mL 3.7968 mL
30 mM 0.1266 mL 0.6328 mL 1.2656 mL 3.1640 mL
40 mM 0.0949 mL 0.4746 mL 0.9492 mL 2.3730 mL
50 mM 0.0759 mL 0.3797 mL 0.7594 mL 1.8984 mL
60 mM 0.0633 mL 0.3164 mL 0.6328 mL 1.5820 mL
80 mM 0.0475 mL 0.2373 mL 0.4746 mL 1.1865 mL
100 mM 0.0380 mL 0.1898 mL 0.3797 mL 0.9492 mL
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Nortriptyline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nortriptyline72-69-5Desmethylamitriptyline DesitriptilinaAutophagyDrug MetaboliteApoptosisdepressionmetabolitetricyclic antidepressanttumorInhibitorinhibitorinhibit

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