PF-04418948

Name: PF-04418948
Brand: MedChemExpress
SKU: HY-18966
Rating: 5.0 (6 reviews)

Cat. No.: HY-18966 Purity: 99.25%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

PF-04418948 is an orally active, potent and selective prostaglandin EP₂ receptor antagonist with an IC₅₀ of 16 nM.

For research use only. We do not sell to patients.

PF-04418948 Chemical Structure

CAS No. : 1078166-57-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 84	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 84	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 36	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 77	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 115	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 484	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 6 publication(s) in Google Scholar

5 Publications Citing Use of MCE PF-04418948

: PF-04418948 purchased from MedChemExpress. Usage Cited in: J Virol. 2018 Sep 26;92(20):e01018-18. [Abstract]; A549 cells are added with an EP2 or an EP4 inhibitor. After 48 h, cells are subjected to WB with an anti-Axl antibody.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

PF-04418948 is an orally active, potent and selective prostaglandin EP₂ receptor antagonist with an IC₅₀ of 16 nM^[1].

IC₅₀ & Target

EP2

16 nM (IC₅₀)

In Vitro

PF-04418948 (2 μM; 90 min) inhibits prostaglandin E2 (PGE2)-induced increase in cAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	CHO cells
Concentration:	2 μM
Incubation Time:	90 min
Result:	Inhibited prostaglandin E2 (PGE2)-induced increase in cAMP in cells expressing EP2 receptors with a functional K_B value of 1.8 nM.

In Vivo

PF-04418948 (oral gavage; 1, 3, and 10 mg/kg; once) attenuats the butaprost-induced cutaneous blood flow response^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague Dawley rats^[1]
Dosage:	1, 3, and 10 mg/kg
Administration:	Oral gavage; 1, 3, and 10 mg/kg; once
Result:	Reduced the peak and AUC butaprost-induced cutaneous blood flow response in a dose-dependent fashion.

Clinical Trial

Molecular Weight

409.41

Formula

C₂₃H₂₀FNO₅

CAS No.

1078166-57-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1(COC2=CC=C3C=C(OC)C=CC3=C2)CN(C(C4=CC=C(F)C=C4)=O)C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (122.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4425 mL	12.2127 mL	24.4254 mL
5 mM	0.4885 mL	2.4425 mL	4.8851 mL
10 mM	0.2443 mL	1.2213 mL	2.4425 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: ≥ 6.5 mg/mL (15.88 mM); Clear solution
Protocol 2

Add each solvent one by one: 2% DMSO 40% PEG300 5% Tween-80 53% Saline
Solubility: ≥ 2.6 mg/mL (6.35 mM); Clear solution
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 4

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 5

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.25%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (252 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. af Forselles KJ, et al. In vitro and in vivo characterization of PF-04418948, a novel, potent and selective prostaglandin EP2 receptor antagonist. Br J Pharmacol. 2011 Dec;164(7):1847-1856. [Content Brief]

[2]. Birrell MA, et al. Selectivity profiling of the novel EP2 receptor antagonist, PF-04418948, in functional bioassay systems: atypical affinity at the guinea pig EP2 receptor. Br J Pharmacol. 2013 Jan;168(1):129-138. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4425 mL	12.2127 mL	24.4254 mL	61.0635 mL
	5 mM	0.4885 mL	2.4425 mL	4.8851 mL	12.2127 mL
	10 mM	0.2443 mL	1.2213 mL	2.4425 mL	6.1063 mL
	15 mM	0.1628 mL	0.8142 mL	1.6284 mL	4.0709 mL
	20 mM	0.1221 mL	0.6106 mL	1.2213 mL	3.0532 mL
	25 mM	0.0977 mL	0.4885 mL	0.9770 mL	2.4425 mL
	30 mM	0.0814 mL	0.4071 mL	0.8142 mL	2.0354 mL
	40 mM	0.0611 mL	0.3053 mL	0.6106 mL	1.5266 mL
	50 mM	0.0489 mL	0.2443 mL	0.4885 mL	1.2213 mL
	60 mM	0.0407 mL	0.2035 mL	0.4071 mL	1.0177 mL
	80 mM	0.0305 mL	0.1527 mL	0.3053 mL	0.7633 mL
	100 mM	0.0244 mL	0.1221 mL	0.2443 mL	0.6106 mL

PF-04418948 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PF-044189481078166-57-0PF04418948PF 04418948Prostaglandin Receptororally activityCHOInhibitorinhibitorinhibit

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PF-04418948

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PF-04418948 Related Antibodies

5 Publications Citing Use of MCE PF-04418948

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•Related Screening Libraries:

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View All Prostaglandin Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

PF-04418948 Related Antibodies

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Complete Stock Solution Preparation Table

PF-04418948 Related Classifications

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