2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. Pralidoxime chloride

Pralidoxime chloride  (Synonyms: 2-PAM chloride)

Cat. No.: HY-B1200 Purity: 99.73%
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Pralidoxime chloride is a potent reactivator of acetylcholinesterase (AChE). Pralidoxime chloride reactivates nerve agent-inhibited AChE via direct nucleophilic attack by the oxime moiety on the phosphorus center of the bound nerve agent. Pralidoxime chloride is an antidote for organophosphate poisoning.

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Pralidoxime chloride Chemical Structure

Pralidoxime chloride Chemical Structure

CAS No. : 51-15-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
100 mg USD 60 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of Pralidoxime chloride:

Pralidoxime chloride Related Antibodies

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Description

Pralidoxime chloride is a potent reactivator of acetylcholinesterase (AChE). Pralidoxime chloride reactivates nerve agent-inhibited AChE via direct nucleophilic attack by the oxime moiety on the phosphorus center of the bound nerve agent. Pralidoxime chloride is an antidote for organophosphate poisoning[1][2].

IC50 & Target

AChE

 

In Vivo

Pralidoxime chloride (10-150 mg/kg; intramuscular administration, once) reverses paraoxon-induced respiratory toxicity in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: F1B6D2 mice (male, administered subcutaneously diethylparaoxon)[3]
Dosage: 10, 50, 100, and 150 mg/kg
Administration: Intramuscular administration, once
Result: Induced a partial, albeit complete, reversal of respiratory toxicity at 50 mg/kg, and completely reversed diethylparaoxon-induced respiratory toxicity in mice at 150 mg/kg.
Clinical Trial
Molecular Weight

172.61

Formula

C7H9ClN2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[N+]1=CC=CC=C1/C=N/O.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (579.34 mM)

DMSO : 21.67 mg/mL (125.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7934 mL 28.9670 mL 57.9341 mL
5 mM 1.1587 mL 5.7934 mL 11.5868 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.17 mg/mL (12.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.17 mg/mL (12.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 5.7934 mL 28.9670 mL 57.9341 mL 144.8352 mL
5 mM 1.1587 mL 5.7934 mL 11.5868 mL 28.9670 mL
10 mM 0.5793 mL 2.8967 mL 5.7934 mL 14.4835 mL
15 mM 0.3862 mL 1.9311 mL 3.8623 mL 9.6557 mL
20 mM 0.2897 mL 1.4484 mL 2.8967 mL 7.2418 mL
25 mM 0.2317 mL 1.1587 mL 2.3174 mL 5.7934 mL
30 mM 0.1931 mL 0.9656 mL 1.9311 mL 4.8278 mL
40 mM 0.1448 mL 0.7242 mL 1.4484 mL 3.6209 mL
50 mM 0.1159 mL 0.5793 mL 1.1587 mL 2.8967 mL
60 mM 0.0966 mL 0.4828 mL 0.9656 mL 2.4139 mL
80 mM 0.0724 mL 0.3621 mL 0.7242 mL 1.8104 mL
100 mM 0.0579 mL 0.2897 mL 0.5793 mL 1.4484 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pralidoxime chloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pralidoxime51-15-02-PAMCholinesterase (ChE)reactivatordiethylparaoxonpralidoximeplethymosgraphyorganophosphatesnerve agentorganophosphorusInhibitorinhibitorinhibit

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