2. Membrane Transporter/Ion Channel Autophagy
  3. GLUT Autophagy
  4. STF-31

STF-31 is a selective inhibitor of glucose transporter 1 (GLUT1), with an IC50 of 1 μM. STF-31 is also a NAMPT inhibitor. STF-31 inhibits glucose uptake in renal cell carcinoma (RCC) 4 cells.

For research use only. We do not sell to patients.

STF-31 Chemical Structure

STF-31 Chemical Structure

CAS No. : 724741-75-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 160 In-stock
Solution
10 mM * 1 mL in DMSO USD 160 In-stock
Solid
1 mg USD 37 In-stock
5 mg USD 93 In-stock
10 mg USD 145 In-stock
50 mg USD 581 In-stock
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Customer Review

Based on 12 publication(s) in Google Scholar

STF-31 Related Antibodies

Top Publications Citing Use of Products

View All GLUT Isoform Specific Products:

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GLUT1 GLUT2 GLUT3 GLUT4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

STF-31 is a selective inhibitor of glucose transporter 1 (GLUT1), with an IC50 of 1 μM. STF-31 is also a NAMPT inhibitor. STF-31 inhibits glucose uptake in renal cell carcinoma (RCC) 4 cells[1][2].

IC50 & Target[2]

GLUT1

1 μM (IC50)

In Vitro

STF-31 (0.01-10 μM; 10 days) is specifically toxic to RCC4 cells, whereas RCC4/VHL cells are relatively unaffected. RCC4/VHL cells treated with STF-31 (5 μM; 10 days) largely recovery, whereas RCC4 cells under the same conditions does not[1].
STF-31 (1.25-5 μM; 3 days) does not induce autophagy, apoptosis, or DNA damage. STF-31 causes a necrotic cell death[1].
STF-31 reduces the efferocytosis of wild type and SLC2A1-overexpressing LR73 cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

STF-31 Related Antibodies

Cell Viability Assay[1]

Cell Line: Wild-type RCC4 cells (RCC4/VHL) and RCC4 cells without VHL
Concentration: 0.01, 0.1, 1, 10 μM
Incubation Time: 10 days
Result: Reduced viability of RCC4 cells without VHL in a concentration-dependent manner when compared to their wild-type counterparts.
In Vivo

STF-31 (10 mg/kg; i.p.; twice daily for 2 days, followed by once daily for another 3 days) does not affect normal mice body weight, behavior, and ERG responses. STF-31 reduces light-induced CX3CR1gfp/+ mice microglial activation and retinal degeneration[3].
STF-31 (10 mg/kg; i.p.) promotes accumulation of necrotic thymocytes after Dexamethasone-induced apoptosis in vivo[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Twelve-week old C57BL/6J and CX3CR1gfp/+ mice[3]
Dosage: 10 mg/kg
Administration: I.p. injections; twice daily for 2 days, followed by once daily for another 3 days
Result: Improved photoreceptor survival and reduced microglial activation of CX3CR1gfp/+ mice, and not induced any C57BL/6J mice retinal cell death.
Molecular Weight

423.53

Formula

C23H25N3O3S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=CC=CN=C1)C2=CC=C(CNS(=O)(C3=CC=C(C(C)(C)C)C=C3)=O)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 34 mg/mL (80.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3611 mL 11.8055 mL 23.6111 mL
5 mM 0.4722 mL 2.3611 mL 4.7222 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (5.90 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.01%

COA (246 KB) HNMR (421 KB) LCMS (138 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3611 mL 11.8055 mL 23.6111 mL 59.0277 mL
5 mM 0.4722 mL 2.3611 mL 4.7222 mL 11.8055 mL
10 mM 0.2361 mL 1.1806 mL 2.3611 mL 5.9028 mL
15 mM 0.1574 mL 0.7870 mL 1.5741 mL 3.9352 mL
20 mM 0.1181 mL 0.5903 mL 1.1806 mL 2.9514 mL
25 mM 0.0944 mL 0.4722 mL 0.9444 mL 2.3611 mL
30 mM 0.0787 mL 0.3935 mL 0.7870 mL 1.9676 mL
40 mM 0.0590 mL 0.2951 mL 0.5903 mL 1.4757 mL
50 mM 0.0472 mL 0.2361 mL 0.4722 mL 1.1806 mL
60 mM 0.0394 mL 0.1968 mL 0.3935 mL 0.9838 mL
80 mM 0.0295 mL 0.1476 mL 0.2951 mL 0.7378 mL
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STF-31 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STF-31724741-75-7STF31STF 31GLUTAutophagyGlucose transporterinhibitorglucoseGLUT1RCC4/VHLRCC4VHLInhibitorinhibit

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