2. Autophagy NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Autophagy Reactive Oxygen Species
  4. Schisandrin B

Schisandrin B  (Synonyms: γ-Schisandrin; Wuweizisu B)

Cat. No.: HY-N0089 Purity: 99.94%
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Schisandrin B (γ-Schisandrin) is a biphenylcyclooctadiene derivative isolated from Schisandra chinensis and has been shown to have antioxidant effects on the liver and heart of rodents.

For research use only. We do not sell to patients.

Schisandrin B Chemical Structure

Schisandrin B Chemical Structure

CAS No. : 61281-37-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 107 In-stock
Solution
10 mM * 1 mL in DMSO USD 107 In-stock
Solid
5 mg USD 98 In-stock
10 mg USD 158 In-stock
50 mg USD 527 In-stock
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Based on 9 publication(s) in Google Scholar

Other Forms of Schisandrin B:

Schisandrin B Related Antibodies

Top Publications Citing Use of Products

    Schisandrin B purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2020 Jul 31;11:1175.  [Abstract]

    BMMs are cultured with or without 20 μM Schisandrin B in the presence of M-CSF and RANKL for the indicated time periods. The inhibitory effects of Schisandrin B on osteoclast-related genes expression are further confirmed by Western blot analysis, which shows that Schisandrin B dramatically suppresses the protein expression of these target genes during osteoclast differentiation.

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    • Purity & Documentation

    • References

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    Description

    Schisandrin B (γ-Schisandrin) is a biphenylcyclooctadiene derivative isolated from Schisandra chinensis and has been shown to have antioxidant effects on the liver and heart of rodents.

    Cellular Effect
    Cell Line Type Value Description References
    HEK-293T IC50
    7.3 μM
    Compound: 47
    Inhibition of flag-tagged ATR expressed in HEK293T cells using PHAS-1 as substrate after 20 mins by autoradiography
    Inhibition of flag-tagged ATR expressed in HEK293T cells using PHAS-1 as substrate after 20 mins by autoradiography
    		[PMID: 22835870]
    In Vitro

    Schisandrin B modulates cellular redox status and activates Nrf2 and its dependent genes in lymphocytes[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Schisandrin B Related Antibodies

    Real Time qPCR[1]

    Cell Line: lymphocyte
    Concentration: 10-50 μM
    Incubation Time: 4 h
    Result: Increase in relative mRNA copy number of Nrf2, HO-1, TrxR1, and GCLC.
    Increased basal ROS levels and decreased GSH/GSSG ratio.
    In Vivo

    Schisandrin B decreases responsiveness to Con A and anti-CD3/CD28 mAb stimulation[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Mice endotoxic shock model[1]
    Dosage: 80 mg/kg
    Administration: Intraperitoneally injected (i.p.), single dose
    Result: Decreased proliferation and secretion of the proinflammatory cytokines IL-2, IL-6, and IFN-g .
    Molecular Weight

    400.46

    Formula

    C23H28O6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC(C1)C(C)CC2=CC(OC)=C(OC)C(OC)=C2C3=C1C=C4OCOC4=C3OC
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (249.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4971 mL 12.4856 mL 24.9713 mL
    5 mM 0.4994 mL 2.4971 mL 4.9943 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.24 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.24 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.94%

    SDS (393 KB)

    COA (260 KB) LCMS (97 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4971 mL 12.4856 mL 24.9713 mL 62.4282 mL
    5 mM 0.4994 mL 2.4971 mL 4.9943 mL 12.4856 mL
    10 mM 0.2497 mL 1.2486 mL 2.4971 mL 6.2428 mL
    15 mM 0.1665 mL 0.8324 mL 1.6648 mL 4.1619 mL
    20 mM 0.1249 mL 0.6243 mL 1.2486 mL 3.1214 mL
    25 mM 0.0999 mL 0.4994 mL 0.9989 mL 2.4971 mL
    30 mM 0.0832 mL 0.4162 mL 0.8324 mL 2.0809 mL
    40 mM 0.0624 mL 0.3121 mL 0.6243 mL 1.5607 mL
    50 mM 0.0499 mL 0.2497 mL 0.4994 mL 1.2486 mL
    60 mM 0.0416 mL 0.2081 mL 0.4162 mL 1.0405 mL
    80 mM 0.0312 mL 0.1561 mL 0.3121 mL 0.7804 mL
    100 mM 0.0250 mL 0.1249 mL 0.2497 mL 0.6243 mL
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    Schisandrin B Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Schisandrin B61281-37-6γ-Schisandrin Wuweizisu BAutophagyReactive Oxygen SpeciesInhibitorinhibitorinhibit

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