2. GPCR/G Protein Neuronal Signaling
  3. Dopamine Receptor
  4. Sulpiride

Sulpiride is an orally active dopamine D2/D3 receptor antagonist. Sulpiride is an atypical antipsychotic agent of the benzamide family. Sulpiride can be used in research into anxiety, depression and breast cancer.

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Sulpiride Chemical Structure

Sulpiride Chemical Structure

CAS No. : 15676-16-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 7 publication(s) in Google Scholar

Other Forms of Sulpiride:

Sulpiride Related Antibodies

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  • Biological Activity

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Description

Sulpiride is an orally active dopamine D2/D3 receptor antagonist. Sulpiride is an atypical antipsychotic agent of the benzamide family. Sulpiride can be used in research into anxiety, depression and breast cancer[1][2][3].

IC50 & Target[2]

D2 Receptor

 

D3 Receptor

 

Cellular Effect
Cell Line Type Value Description References
CHO-K1 IC50
560 nM
Compound: Sulpiride
Antagonist activity at human dopamine D3 receptor expressed in CHO-K1 cells assessed as inhibition of dopamine-induced recruitment of beta-arrestin-2 after 90 mins by beta-galactosidase assay
Antagonist activity at human dopamine D3 receptor expressed in CHO-K1 cells assessed as inhibition of dopamine-induced recruitment of beta-arrestin-2 after 90 mins by beta-galactosidase assay
[PMID: 21495689]
In Vitro

Dopamine, Alzheimer's, depression, Anxiety, Sprague-Dawley rats, NMRI mouse, Parkinson’s disease, psychosis, MCF-7/Adr, Breast cancer, Dexamethasone, Drug combination，MCF-7/Adr, Cancer stem-like cells (CSCs)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sulpiride Related Antibodies
In Vivo

Sulpiride (50 mg/kg; Subcutaneous injection; Single dose) can increase the dopamine level in Sprague-Dawley rats[2].
Sulpiride (5-20 mg/kg; Intraperitoneal injection; Single dose) improves memory, but induces anxiety in Parkinson's disease (PD) mice at a dose of 20 mg/kg[3].
Sulpiride (25-100 mg/kg; Oral gavage; Once daily for 18 days) can enhance the anti-tumor activity of dexamethasone (DEX) (HY-14648) (1 and 8 mg/kg) in combination with dexamethasone in nu/nu nude mice bearing MCF-7/Adr xenografts.Without significant toxicity to organs[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague-Dawley rats model[2]
Dosage: 50 mg/kg
Administration: Subcutaneous injection (s.c.); Single dose.
Result: Caused dopamine concentrations in the dialysates to roughly double.
Animal Model: 6-hydroxydopamine (6-OHDA) male NMRI mouse model of Parkinson’s disease (PD)[3]
Dosage: 5 mg/kg, 10 mg/kg, 20 mg/kg
Administration: Intraperitoneal injection (i.p.); Single dose
Result: Decreased %OAT (open-arm time) but had no significant effect on %OAE (open-arm entries) and locomotor activity.
Animal Model: Female nu/nu nude mouse models of breast cancer bearing MCF-7/Adr xenografts[4]
Dosage: 25 mg/kg, 50 mg/kg, 100 mg/kg
Administration: Oral gavage (p.o.); Once daily for 18 days. In combination with DEX (1 and 10 mg/kg; p.o.; Once daily for 18 days)
Result: When used in combination with DEX (1 mg/kg) at 50 m/kg, enhanced the tumor suppression rate (14.4%).
When combined with DEX (8 mg/kg) at 25, 50, and 100 mg/kg, enhanced the tumor suppression rate by 30.2%, 27.55%, and 14.97%, respectively.
Clinical Trial
Molecular Weight

341.43

Formula

C15H23N3O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCC1N(CC)CCC1)C2=CC(S(=O)(N)=O)=CC=C2OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (292.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9289 mL 14.6443 mL 29.2886 mL
5 mM 0.5858 mL 2.9289 mL 5.8577 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9289 mL 14.6443 mL 29.2886 mL 73.2215 mL
5 mM 0.5858 mL 2.9289 mL 5.8577 mL 14.6443 mL
10 mM 0.2929 mL 1.4644 mL 2.9289 mL 7.3221 mL
15 mM 0.1953 mL 0.9763 mL 1.9526 mL 4.8814 mL
20 mM 0.1464 mL 0.7322 mL 1.4644 mL 3.6611 mL
25 mM 0.1172 mL 0.5858 mL 1.1715 mL 2.9289 mL
30 mM 0.0976 mL 0.4881 mL 0.9763 mL 2.4407 mL
40 mM 0.0732 mL 0.3661 mL 0.7322 mL 1.8305 mL
50 mM 0.0586 mL 0.2929 mL 0.5858 mL 1.4644 mL
60 mM 0.0488 mL 0.2441 mL 0.4881 mL 1.2204 mL
80 mM 0.0366 mL 0.1831 mL 0.3661 mL 0.9153 mL
100 mM 0.0293 mL 0.1464 mL 0.2929 mL 0.7322 mL
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Sulpiride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulpiride15676-16-1Dopamine ReceptorDopamineAlzheimer'sdepressionAnxietySprague-Dawley ratsNMRI mouseParkinson’s diseasepsychosisMCF-7/AdrBreast cancerDexamethasoneDrug combinationCancer stem-like cells (CSCs)Inhibitorinhibitorinhibit

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