2. Apoptosis Metabolic Enzyme/Protease Cell Cycle/DNA Damage Epigenetics Autophagy
  3. MDM-2/p53 Dihydroorotate Dehydrogenase Sirtuin Autophagy
  4. Tenovin-6 Hydrochloride

Tenovin-6 Hydrochloride, an analog of Tenovin-1 (HY-13423), is an activator of p53 transcriptional activity. Tenovin-6 Hydrochloride inhibits the protein deacetylase activities of purified human SIRT1, SIRT2, and SIRT3 with IC50s of 21 μM, 10 μM, and 67 μM, respectively. Tenovin-6 Hydrochloride also inhibits dihydroorotate dehydrogenase (DHODH).

For research use only. We do not sell to patients.

Tenovin-6 Hydrochloride Chemical Structure

Tenovin-6 Hydrochloride Chemical Structure

CAS No. : 1011301-29-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 86 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Tenovin-6 Hydrochloride:

Tenovin-6 Hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Tenovin-6 Hydrochloride purchased from MedChemExpress. Usage Cited in: J Nutr. 2020 Jul 1;150(7):1731-1737.  [Abstract]

    The cell viability in untreated SH-SY5Y cells or those stimulated with Tenovin-6 and then treated with MPP+ or the combination of MPP+ and CPO-A.

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    Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7

    • Biological Activity

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    • Purity & Documentation

    • References

    • Customer Review

    Description

    Tenovin-6 Hydrochloride, an analog of Tenovin-1 (HY-13423), is an activator of p53 transcriptional activity. Tenovin-6 Hydrochloride inhibits the protein deacetylase activities of purified human SIRT1, SIRT2, and SIRT3 with IC50s of 21 μM, 10 μM, and 67 μM, respectively. Tenovin-6 Hydrochloride also inhibits dihydroorotate dehydrogenase (DHODH)[1][2].

    IC50 & Target[1]

    SIRT2

    10 μM (IC50)

    SIRT1

    21 μM (IC50)

    SIRT3

    67 μM (IC50)

    HDAC8

     

    MDM-2/p53

     

    In Vitro

    Tenovin-6 Hydrochloride inhibits the growth of S. cerevisiae cultures with an IC50 of 30 μM and is more toxic to yeast than the less water-soluble tenovin-1. Tenovin-6 Hydrochloride rapidly increases the levels of endogenous K382-Ac p53 in MCF-7 cells[1].
    Tenovin-6 Hydrochloride (0 to 15 μM) dose dependently increases the level of LC3-II in diverse cell types, and the increase is ATG5/7 dependent. Tenovin-6 Hydrochloride treatment also increases the number and intensity of autophagic vesicles with or without the presence of Torin 1, and prevents Torin 1-induced SQSTM1/p62 degradation. Tenovin-6 Hydrochloride affects the acidification of autolysosomes and impairs the hydrolytic activity of lysosomes but does not affect the fusion between autophagosomes and lysosomes. That Tenovin-6 Hydrochloride inhibits autophagy does not correlate with p53 activation and SIRT1/2 inhibition by knockdown or knockout cannot mimic the effect of Tenovin-6 Hydrochloride on LC3B accumulation[3].
    Tenovin-6 Hydrochloride (0, 1, 2.5, 5 or 10 μM) potently inhibits cell proliferation in a dose- and time-dependent manner in all OCI-Ly1, DHL-10, U2932, RIVA, HBL1 and OCI-Ly10 cell lines. Tenovin-6 Hydrochloride consistently increases LC3B-II level in DLBCL cell lines by inhibiting the classical autophagy pathway, without activating p53, and the increase is independent of SIRT1/2/3 and p53. Tenovin-6 Hydrochloride induces apoptosis through the extrinsic cell-death pathway[4.
    Tenovin-6 Hydrochloride suppresses the growth of UM cells with IC50 of 12.8 μM, 11.0 μM, 14.58 μM and 9.62 μM for 92.1, Mel 270, Omm 1 and Omm 2.3 cells, respectively    [5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tenovin-6 Hydrochloride Related Antibodies
    In Vivo

    Tenovin-6 Hydrochloride (50 mg/kg, i.p.) inhibits the growth of tumor in mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    491.09

    Formula

    C25H35ClN4O2S
    CAS No.
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    O=C(NC(NC1=CC=C(NC(CCCCN(C)C)=O)C=C1)=S)C2=CC=C(C(C)(C)C)C=C2.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 49 mg/mL (99.78 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0363 mL 10.1814 mL 20.3629 mL
    5 mM 0.4073 mL 2.0363 mL 4.0726 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

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    (per animal)

    μL

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References
    Kinase Assay
    [1]

    Assays are carried out using purified components in the Fluor de Lys Fluorescent Assay Systems. Relevant FdL substrates are used at 7 μM and NAD+ at 1 mM. Tenovins are solubilized in DMSO with the final DSMO concentration in the reaction being less than 0.25%. For SirT1 and HDAC8, one unit of enzyme is used per reaction, and for SirT2 and SirT3, five units is used per reaction. Reactions are carried out at 37°C for 1 hr.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [4]

    The MTS assay is used to evaluate cell viability. UM cells are seeded into each well of 96-well plates (5,000 cells/well) and treated the next day with control or Tenovin-6 in an increasing concentrations from 0 to 20 μM for 68 h, and then MTS is added at 20 μL/well to be read at a wave length of 490 nm, the IC50 is determined by curve fitting of the sigmoidal dose-response curve.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Female SCID mice are injected subcutaneously with 1×106 ARN8 cells suspended in matrigel. Tumors are allowed to reach a size of approximately 10 mm3. Tenovin-6 is administered daily at 50 mg/kg by intraperitoneal injection. Control animals are treated with vehicle solution containing cyclodextrin 20% (w/v) and DMSO 10% (v/v). Tumor diameters are measured using calipers, and volumes are calculated using the equation V=π4/3[(d1 + d2)/4]3. Median values of tumor size are calculated for each time point as well as the corresponding 95% confidence intervals. Comparison of control and drug-treated tumor size distributions are made by Mann-Whitney U-test. An alpha-level of 0.05 is considered appropriate for determination of statistical significance.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0363 mL 10.1814 mL 20.3629 mL 50.9072 mL
    5 mM 0.4073 mL 2.0363 mL 4.0726 mL 10.1814 mL
    10 mM 0.2036 mL 1.0181 mL 2.0363 mL 5.0907 mL
    15 mM 0.1358 mL 0.6788 mL 1.3575 mL 3.3938 mL
    20 mM 0.1018 mL 0.5091 mL 1.0181 mL 2.5454 mL
    25 mM 0.0815 mL 0.4073 mL 0.8145 mL 2.0363 mL
    30 mM 0.0679 mL 0.3394 mL 0.6788 mL 1.6969 mL
    40 mM 0.0509 mL 0.2545 mL 0.5091 mL 1.2727 mL
    50 mM 0.0407 mL 0.2036 mL 0.4073 mL 1.0181 mL
    60 mM 0.0339 mL 0.1697 mL 0.3394 mL 0.8485 mL
    80 mM 0.0255 mL 0.1273 mL 0.2545 mL 0.6363 mL
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    Tenovin-6 Hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tenovin-61011301-29-3Tenovin6Tenovin 6MDM-2/p53Dihydroorotate DehydrogenaseSirtuinAutophagyDHODHMDM2protein-deacetylatingacetylationdegradationInhibitorinhibitorinhibit

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