Tetracycline hydrochloride

Name: Tetracycline hydrochloride
Brand: MedChemExpress
SKU: HY-B0474
Rating: 5.0 (33 reviews)

Cat. No.: HY-B0474 Purity: 99.67%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tetracycline (hydrochloride) is a broad-spectrum antibiotic, exhibiting activity against a wide range of gram-positive and gram-negative bacteria.

For research use only. We do not sell to patients.

Tetracycline hydrochloride Chemical Structure

CAS No. : 64-75-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g	USD 72	In-stock	Estimated Time of Arrival: December 31
5 g	USD 108	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Customer Review

Based on 33 publication(s) in Google Scholar

Other Forms of Tetracycline hydrochloride:

Tetracycline In-stock
Tetracycline hydrochloride (Standard) Get quote

33 Publications Citing Use of MCE Tetracycline hydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Tetracycline (hydrochloride) is a broad-spectrum antibiotic, exhibiting activity against a wide range of gram-positive and gram-negative bacteria.

IC₅₀ & Target

Tetracycline

In Vitro

Tetracyclines are broad-spectrum agents, exhibiting activity against a wide range of gram-positive and gram-negative bacteria, atypical organisms such as chlamydiae, mycoplasmas, and rickettsiae, and protozoan parasites. Tetracyclines inhibit bacterial protein synthesis by preventing the association of aminoacyl-tRNA with the bacterial ribosome. Tetracyclines traverse the outer membrane of gram-negative enteric bacteria through the OmpF and OmpC porin channels, as positively charged cation (probably magnesium)-tetracycline coordination complexes ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The tetracyclines have applications for the treatment of infections in poultry, cattle, sheep, and swine. In some cases, e.g., for therapeutic treatment of large numbers of poultry reared on commercial farms, the antibiotics are added directly to feed or water or can be administered in aerosols. Tetracyclines could be used as growth promotion or growth enhancement. Tetracyclines are used in aquaculture to control infections in salmon, catfish, and lobsters^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

480.90

Formula

C₂₂H₂₅ClN₂O₈

CAS No.

64-75-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O[C@@]1(C(C(C(N)=O)=C2O)=O)[C@@]([C@@H]2N(C)C)([H])C[C@@]([C@](O)(C3=CC=C4)C)([H])C(C(C3=C4O)=O)=C1O.Cl

Structure Classification

Initial Source

Microorganisms

Streptomyces

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (129.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (103.97 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0794 mL	10.3972 mL	20.7943 mL
5 mM	0.4159 mL	2.0794 mL	4.1589 mL
10 mM	0.2079 mL	1.0397 mL	2.0794 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.67%

Select Batch:

Data Sheet (295 KB) SDS (418 KB)

COA (255 KB) HNMR (307 KB) RP-HPLC (243 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Chopra I, et al.Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology ofbacterial resistance.Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0794 mL	10.3972 mL	20.7943 mL	51.9859 mL
	5 mM	0.4159 mL	2.0794 mL	4.1589 mL	10.3972 mL
	10 mM	0.2079 mL	1.0397 mL	2.0794 mL	5.1986 mL
	15 mM	0.1386 mL	0.6931 mL	1.3863 mL	3.4657 mL
	20 mM	0.1040 mL	0.5199 mL	1.0397 mL	2.5993 mL
	25 mM	0.0832 mL	0.4159 mL	0.8318 mL	2.0794 mL
	30 mM	0.0693 mL	0.3466 mL	0.6931 mL	1.7329 mL
	40 mM	0.0520 mL	0.2599 mL	0.5199 mL	1.2996 mL
	50 mM	0.0416 mL	0.2079 mL	0.4159 mL	1.0397 mL
	60 mM	0.0347 mL	0.1733 mL	0.3466 mL	0.8664 mL
	80 mM	0.0260 mL	0.1300 mL	0.2599 mL	0.6498 mL
	100 mM	0.0208 mL	0.1040 mL	0.2079 mL	0.5199 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.