(S)-Malic acid (Synonyms: (S)-Hydroxybutanedioic acid; (S)-E 296; L-Malic acid)

Name: (S)-Malic acid
Brand: MedChemExpress
SKU: HY-Y1069
Rating: 4.5 (1 reviews)

Cat. No.: HY-Y1069 Purity: 99.62%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

(S)-Malic acid ((S)-2-Hydroxysuccinic acid) is a dicarboxylic acid in naturally occurring form, contributes to the pleasantly sour taste of fruits and is used as a food additive.

For research use only. We do not sell to patients.

(S)-Malic acid Chemical Structure

CAS No. : 97-67-6

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Based on 1 publication(s) in Google Scholar

Other Forms of (S)-Malic acid:

1 Publications Citing Use of MCE (S)-Malic acid

•SSRN. 2024 Feb 14.

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Biological Activity
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References
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Description

(S)-Malic acid ((S)-2-Hydroxysuccinic acid) is a dicarboxylic acid in naturally occurring form, contributes to the pleasantly sour taste of fruits and is used as a food additive.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

It is showed that ME is essential for (S)-2-Hydroxysuccinic acid (L-malic acid) utilization in L. casei. Furthermore, deletion of either the gene encoding the histidine kinase or the response regulator of the TC system resulted in the loss of the ability to grow on (S)-2-Hydroxysuccinic acid, thus indicating that the cognate TC system regulates and is essential for the expression of ME. Transcriptional analyses shows that expression of maeE is induced in the presence of (S)-2-Hydroxysuccinic acid and repressed by glucose, whereas TC system expression is induced by (S)-2-Hydroxysuccinic acid and is not repressed by glucose^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

134.09

Formula

C₄H₆O₅

CAS No.

97-67-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@@H](O)CC(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (745.77 mM; Need ultrasonic)

DMSO : 100 mg/mL (745.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.4577 mL	37.2884 mL	74.5768 mL
5 mM	1.4915 mL	7.4577 mL	14.9154 mL
10 mM	0.7458 mL	3.7288 mL	7.4577 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (18.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB) HNMR (244 KB) RP-HPLC (253 KB) MS (67 KB)

Handling Instructions (2659 KB)

References

[1]. Landete JM, et al. Requirement of the Lactobacillus casei MaeKR two-component system for L-malic acid utilization via a malic enzyme pathway. Appl Environ Microbiol. 2010 Jan;76(1):84-95. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	7.4577 mL	37.2884 mL	74.5768 mL	186.4419 mL
	5 mM	1.4915 mL	7.4577 mL	14.9154 mL	37.2884 mL
	10 mM	0.7458 mL	3.7288 mL	7.4577 mL	18.6442 mL
	15 mM	0.4972 mL	2.4859 mL	4.9718 mL	12.4295 mL
	20 mM	0.3729 mL	1.8644 mL	3.7288 mL	9.3221 mL
	25 mM	0.2983 mL	1.4915 mL	2.9831 mL	7.4577 mL
	30 mM	0.2486 mL	1.2429 mL	2.4859 mL	6.2147 mL
	40 mM	0.1864 mL	0.9322 mL	1.8644 mL	4.6610 mL
	50 mM	0.1492 mL	0.7458 mL	1.4915 mL	3.7288 mL
	60 mM	0.1243 mL	0.6215 mL	1.2429 mL	3.1074 mL
	80 mM	0.0932 mL	0.4661 mL	0.9322 mL	2.3305 mL
	100 mM	0.0746 mL	0.3729 mL	0.7458 mL	1.8644 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

(S)-Malic acid Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(S)-Malic acid97-67-6(S)-Hydroxybutanedioic acid (S)-E 296 L-Malic acidEndogenous MetaboliteInhibitorinhibitorinhibit

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(S)-Malic acid (Synonyms: (S)-Hydroxybutanedioic acid; (S)-E 296; L-Malic acid)

Customer Review

Other Forms of (S)-Malic acid:

(S)-Malic acid Related Antibodies

1 Publications Citing Use of MCE (S)-Malic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

(S)-Malic acid Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(S)-Malic acid Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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