Aaptamine

Cat. No.: HY-N4225 Purity: 99.71%: FAQs
Data Sheet SDS COA Handling Instructions

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Aaptamine, a spongean alkaloid isolated from a sea sponge Aaptos aaptos, is a competitive antagonist of α-adrenoceptor and activates the p21 promoter in a p53-independent manner.

For research use only. We do not sell to patients.

Aaptamine Chemical Structure

CAS No. : 85547-22-4

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α adrenergic receptor β adrenergic receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Aaptamine, a spongean alkaloid isolated from a sea sponge Aaptos aaptos, is a competitive antagonist of α-adrenoceptor and activates the p21 promoter in a p53-independent manner^[1]^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	EC₅₀	> 100 μM Compound: 1	Inhibition of HIF2alpha in human 786-0 cells expressing truncated HIF1alpha assessed as reduction in luciferase activity after 24 hrs by reporter gene assay Inhibition of HIF2alpha in human 786-0 cells expressing truncated HIF1alpha assessed as reduction in luciferase activity after 24 hrs by reporter gene assay	[PMID: 22928967]
A549	IC₅₀	2.8 μg/mL Compound: 2	Cytotoxicity against human A549 cells after 6 days by MTT assay Cytotoxicity against human A549 cells after 6 days by MTT assay	[PMID: 10514310]
HeLa	IC₅₀	15 μg/mL Compound: 1	Cytotoxicity against human HeLa cells after 3 days by MTT assay Cytotoxicity against human HeLa cells after 3 days by MTT assay	[PMID: 20451377]
HeLa	IC₅₀	19 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by CCK8 assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by CCK8 assay	[PMID: 33170001]
HL-60	IC₅₀	> 55 μM Compound: 7	Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay	[PMID: 12762791]
HT-29	IC₅₀	6.9 μg/mL Compound: 2	Cytotoxicity against human HT-29 cells after 6 days by MTT assay Cytotoxicity against human HT-29 cells after 6 days by MTT assay	[PMID: 10514310]
L5178Y	IC₅₀	8.3 μM Compound: 5	Cytotoxic activity against mouse L5178Y cells by MTT assay Cytotoxic activity against mouse L5178Y cells by MTT assay	[PMID: 23282083]
MCF7	IC₅₀	> 20 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by CCK8 assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by CCK8 assay	[PMID: 33170001]
NB-4	IC₅₀	> 20 μM Compound: 1	Cytotoxicity against human NB4 cells assessed as reduction in cell viability after 48 hrs by CCK8 assay Cytotoxicity against human NB4 cells assessed as reduction in cell viability after 48 hrs by CCK8 assay	[PMID: 33170001]
P388	IC₅₀	0.6 μg/mL Compound: 2	Cytotoxicity against mouse P388 cells after 3 days by MTT assay Cytotoxicity against mouse P388 cells after 3 days by MTT assay	[PMID: 10514310]

Molecular Weight

228.25

Formula

C₁₃H₁₂N₂O₂

CAS No.

85547-22-4

Appearance

Solid

Color

White to yellow

SMILES

COC1=C2C3=C(N=CC=C3C=C1OC)C=CN2

Structure Classification

Initial Source

Animals

sponge

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (438.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3812 mL	21.9058 mL	43.8116 mL
5 mM	0.8762 mL	4.3812 mL	8.7623 mL
10 mM	0.4381 mL	2.1906 mL	4.3812 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.71%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (254 KB) HNMR (203 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Ohizumi Y, et al. Alpha-adrenoceptor blocking action of aaptamine, a novel marine natural product, in vascular smooth muscle. J Pharm Pharmacol. 1984 Nov;36(11):785-6. [Content Brief]

[2]. Aoki S, et al. Aaptamine, a spongean alkaloid, activates p21 promoter in a p53-independent manner. Biochem Biophys Res Commun. 2006 Mar 31;342(1):101-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3812 mL	21.9058 mL	43.8116 mL	109.5290 mL
	5 mM	0.8762 mL	4.3812 mL	8.7623 mL	21.9058 mL
	10 mM	0.4381 mL	2.1906 mL	4.3812 mL	10.9529 mL
	15 mM	0.2921 mL	1.4604 mL	2.9208 mL	7.3019 mL
	20 mM	0.2191 mL	1.0953 mL	2.1906 mL	5.4765 mL
	25 mM	0.1752 mL	0.8762 mL	1.7525 mL	4.3812 mL
	30 mM	0.1460 mL	0.7302 mL	1.4604 mL	3.6510 mL
	40 mM	0.1095 mL	0.5476 mL	1.0953 mL	2.7382 mL
	50 mM	0.0876 mL	0.4381 mL	0.8762 mL	2.1906 mL
	60 mM	0.0730 mL	0.3651 mL	0.7302 mL	1.8255 mL
	80 mM	0.0548 mL	0.2738 mL	0.5476 mL	1.3691 mL
	100 mM	0.0438 mL	0.2191 mL	0.4381 mL	1.0953 mL

Aaptamine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Aaptamine85547-22-4Adrenergic ReceptorBeta ReceptorInhibitorinhibitorinhibit

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