2. Metabolic Enzyme/Protease
  3. HPPD
  4. Benzobicyclon

Benzobicyclon is a 4-HPPD inhibitor and herbicide that reacts with water to form a hydrolysate of the active herbicide benzobicycline, causing bleaching and death of weeds. Benzobicyclon is effective against grass, sedge and broadleaf weeds. Benzobicyclon effectively targets sulfonylurea herbicide-resistant biotypes as well as wild-type weeds.

For research use only. We do not sell to patients.

Benzobicyclon Chemical Structure

Benzobicyclon Chemical Structure

CAS No. : 156963-66-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 88 In-stock
Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Solid
5 mg USD 80 In-stock
10 mg USD 130 In-stock
50 mg USD 350 In-stock
100 mg USD 560 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Benzobicyclon:

Benzobicyclon Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

Benzobicyclon is a 4-HPPD inhibitor and herbicide that reacts with water to form a hydrolysate of the active herbicide benzobicycline, causing bleaching and death of weeds. Benzobicyclon is effective against grass, sedge and broadleaf weeds. Benzobicyclon effectively targets sulfonylurea herbicide-resistant biotypes as well as wild-type weeds[1][2].

IC50 & Target

4-HPPD[1]
In Vitro

Benzobicyclon (100-300 g a.i./ha; 1 L or 2 L) exhibits excellent herbicidal activity against S. juncoides regardless of the emergence depth between 0 and 3 cm and (200-300 g a.i./ha; pre-emergence; 1 L) hardly affected by the soil types[1].
Benzobicyclon (300 g a.i./ha; 1-leaf and 1 L stages) completely controls S. juncoides at 25°C, 20°C, and 15°C, and takes longer to reach 90% control at low temperatures[1].
Benzobicyclon (200, 300 g a.i./ha; pre-emergence and 1 L stages) exhibits excellent herbicidal activity against all SU-R biotypes S. juncoides, M. vaginalis and L. dubia as well as SU-S biotypes. (SU-S: sulfonylurea herbicide-susceptible, SU-R: sulfonylurea herbicide-resistant)[1].
Benzobicyclon (200-600 g a.i./ha; pre-emergence and 1 L stages) exhibits excellent herbicidal activity against E. crus-galli , S. juncoides, M. vaginalis, L. var. dubia and C. serotinus and shows good rice crop safety[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Benzobicyclon Related Antibodies

Cell Viability Assay[1]

Cell Line: S. juncoides
Concentration: 200, 300 g a.i./ha
Incubation Time: pre-emergence and 1 L stages
Result: Completely controlled S. juncoides in 5 types of paddy soils, namely, clay loam, loam, light clay, sandy loam, and heavy clay.

Cell Viability Assay[1]

Cell Line: S. juncoides
Concentration: 300 g a.i./ha
Incubation Time: 1-leaf and 1 L stages
Result: Showed 90% control at 25°C, 20°C, and 15°C at 14, 22, and 32 days after application, respectively.

Cell Viability Assay[1]

Cell Line: S. juncoides, M. vaginalis, L. dubia
Concentration: 200, 300 g a.i./ha
Incubation Time: Pre-emergence and 1 L stages
Result: Showed good herbicidal activity against all SU-R biotypes tested as well as SU-susceptible biotypes.

Cell Viability Assay[1]

Cell Line: Transplanted rice (3 cm)
Concentration: 200-600 g a.i./ha
Incubation Time: Pre-emergence and 1 L stages
Result: Exhibited excellent herbicidal activity and broad-herbicidal spectrum without causing injury to transplanted rice.
Molecular Weight

446.97

Formula

C22H19ClO4S2
CAS No.
Appearance

Solid

Color

Light yellow to brown

SMILES

O=C1C2CC(C(SC3=CC=CC=C3)=C1C(C4=CC=C(C=C4Cl)S(=O)(C)=O)=O)CC2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (37.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2373 mL 11.1864 mL 22.3729 mL
5 mM 0.4475 mL 2.2373 mL 4.4746 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (3.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.25%

SDS (562 KB)

COA (259 KB) HNMR (274 KB) RP-HPLC (244 KB) MS (70 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2373 mL 11.1864 mL 22.3729 mL 55.9322 mL
5 mM 0.4475 mL 2.2373 mL 4.4746 mL 11.1864 mL
10 mM 0.2237 mL 1.1186 mL 2.2373 mL 5.5932 mL
15 mM 0.1492 mL 0.7458 mL 1.4915 mL 3.7288 mL
20 mM 0.1119 mL 0.5593 mL 1.1186 mL 2.7966 mL
25 mM 0.0895 mL 0.4475 mL 0.8949 mL 2.2373 mL
30 mM 0.0746 mL 0.3729 mL 0.7458 mL 1.8644 mL
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Benzobicyclon Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Benzobicyclon156963-66-5HPPD4-Hydroxyphenylpyruvate dioxygenaseherbicide resistancegramineoussedgebroadleaf weedsscirpus juncoidesSU-resistant weedsInhibitorinhibitorinhibit

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