2. Metabolic Enzyme/Protease Apoptosis Immunology/Inflammation
  3. Phospholipase TNF Receptor Interleukin Related
  4. BML-280

BML-280  (Synonyms: VU0285655-1)

Cat. No.: HY-114095 Purity: 99.64%
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BML-280 (VU0285655-1) is a potent and selective phospholipase D2 (PLD2) inhibitor. BML-280 has the ability to prevent caspase-3 cleavage and reduction in cell viability induced by high glucose. BML-280 can be used for rheumatoid arthritis research.

For research use only. We do not sell to patients.

BML-280 Chemical Structure

BML-280 Chemical Structure

CAS No. : 1158347-73-9

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5 mg USD 230 In-stock
10 mg USD 380 In-stock
25 mg USD 790 In-stock
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Based on 1 publication(s) in Google Scholar

BML-280 Related Antibodies

Top Publications Citing Use of Products

View All Phospholipase Isoform Specific Products:

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Phospholipase A PLD Phospholipase C

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TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

View All Interleukin Related Isoform Specific Products:

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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Description

BML-280 (VU0285655-1) is a potent and selective phospholipase D2 (PLD2) inhibitor. BML-280 has the ability to prevent caspase-3 cleavage and reduction in cell viability induced by high glucose. BML-280 can be used for rheumatoid arthritis research[1][2].

IC50 & Target

PLD2

 

PLD1

 

IL-1β

 

IL-8

 

In Vitro

BML-280 shows an approximately 21-fold selectivity for PLD2[3].
BML-280 (0-0.1 μM) suppresses formyl-Met-Leu-Phe (fMLP)-stimulated PLD activity in a concentration dependent manner, with an IC50 of 0.04 ± 0.01 μM[3].
BML-280 (0-0.3 μM) inhibits O2- generation, and the inhibition reaches a plateau (about 20 % inhibition) at around 0.01 μM to 0.3 μM[3].
BML-280 (0-5 μM, 24 h) reduces proliferation in PLD1-deficient cells, but also in PLD2-deficient cells exposed to IGF-1 (Insulin-like growth factor 1)[1].
BML-280 inhibits mRNA levels and secretion of tumor necrosis factor-α, IL-1β and IL-8 in human periodontal ligament cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BML-280 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Wild-type, PLD1- and PLD2-deficient astrocytes
Concentration: 0, 50, 500, and 5000 nM
Incubation Time: 24 h
Result: Had minor effects in wild-type and PLD2-deficient cells, but completely blocked PLD activity in PLD1-deficient cells. Caused a highly significant inhibition of glial proliferation when astrocytes were stimulated by FCS (fetal calf serum) or IGF-1, respectively. Showed non-specific effects because they inhibited cell proliferation even in PLD1/2 double knockouts at 5 µM.
Molecular Weight

429.51

Formula

C25H27N5O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC2=CC=CC=C2N=C1)NCCN(CC3)CCC3(N(C4=CC=CC=C4)CN5)C5=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (291.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3282 mL 11.6412 mL 23.2823 mL
5 mM 0.4656 mL 2.3282 mL 4.6565 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3282 mL 11.6412 mL 23.2823 mL 58.2059 mL
5 mM 0.4656 mL 2.3282 mL 4.6565 mL 11.6412 mL
10 mM 0.2328 mL 1.1641 mL 2.3282 mL 5.8206 mL
15 mM 0.1552 mL 0.7761 mL 1.5522 mL 3.8804 mL
20 mM 0.1164 mL 0.5821 mL 1.1641 mL 2.9103 mL
25 mM 0.0931 mL 0.4656 mL 0.9313 mL 2.3282 mL
30 mM 0.0776 mL 0.3880 mL 0.7761 mL 1.9402 mL
40 mM 0.0582 mL 0.2910 mL 0.5821 mL 1.4551 mL
50 mM 0.0466 mL 0.2328 mL 0.4656 mL 1.1641 mL
60 mM 0.0388 mL 0.1940 mL 0.3880 mL 0.9701 mL
80 mM 0.0291 mL 0.1455 mL 0.2910 mL 0.7276 mL
100 mM 0.0233 mL 0.1164 mL 0.2328 mL 0.5821 mL
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BML-280 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BML-2801158347-73-9VU0285655-1BML280BML 280PhospholipaseTNF ReceptorInterleukin RelatedTumor Necrosis Factor ReceptorTNFRILAstrocytesDimethylsulfoxideFetal calf serumInsulin-like growth factor 1Phorbol esterfMLPInhibitorinhibitorinhibit

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