BMS-8

Name: BMS-8
Brand: MedChemExpress
SKU: HY-116274
Rating: 4.5 (1 reviews)

Cat. No.: HY-116274 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BMS-8 inhibits the PD-1/PD-L1 interaction with IC₅₀ of 7.2 μM. BMS-8, binds directly to PD-L1 and induces formation of PD-L1 homodimers, which in turn prevents the interaction with PD-1.

For research use only. We do not sell to patients.

BMS-8 Chemical Structure

CAS No. : 1675201-90-7

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 345	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

BMS-8 inhibits the PD-1/PD-L1 interaction with IC₅₀ of 7.2 μM. BMS-8, binds directly to PD-L1 and induces formation of PD-L1 homodimers, which in turn prevents the interaction with PD-1^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	6 μM Compound: BMS-8	Antiproliferative activity against human A549 cells incubated for 2 days by MTT assay Antiproliferative activity against human A549 cells incubated for 2 days by MTT assay	[PMID: 30878834]
HEK293	IC₅₀	146 nM Compound: BMS-8	Inhibition of human C-terminal Ig-Fc-tagged PD1 (Leu25 to Gln167 residues) expressed in HEK293 cells/human C-terminal epitope-His-tagged PDL1 (Phe19 to Arg238 residues) expressed in HEK293 cells protein-protein interaction preincubated for 15 mins with PD Inhibition of human C-terminal Ig-Fc-tagged PD1 (Leu25 to Gln167 residues) expressed in HEK293 cells/human C-terminal epitope-His-tagged PDL1 (Phe19 to Arg238 residues) expressed in HEK293 cells protein-protein interaction preincubated for 15 mins with PD	[PMID: 32667799]
HEK293	IC₅₀	60 μM Compound: BMS-8	Antiproliferative activity against HEK293 cells incubated for 2 days by MTT assay Antiproliferative activity against HEK293 cells incubated for 2 days by MTT assay	[PMID: 30878834]
HeLa	IC₅₀	> 100 μM Compound: BMS-8	Antiproliferative activity against human HeLa cells incubated for 2 days by MTT assay Antiproliferative activity against human HeLa cells incubated for 2 days by MTT assay	[PMID: 30878834]
HT-29	IC₅₀	19 μM Compound: BMS-8	Antiproliferative activity against human HT-29 cells incubated for 2 days by MTT assay Antiproliferative activity against human HT-29 cells incubated for 2 days by MTT assay	[PMID: 30878834]
MCF7	IC₅₀	20 μM Compound: BMS-8	Antiproliferative activity against human MCF7 cells incubated for 2 days by MTT assay Antiproliferative activity against human MCF7 cells incubated for 2 days by MTT assay	[PMID: 30878834]

In Vitro

BMS-8 tends to have a more stable binding mode with one PD-L1 monomer than the other and the small-molecule inducing PD-L1 dimerization was further stabilized by the non-polar interaction of Ile54, Tyr56, Met115, Ala121, and Tyr123 on both monomers and the water bridges involved in ALys124^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

494.42

Formula

C₂₇H₂₈BrNO₃

CAS No.

1675201-90-7

Appearance

Solid

Color

White to off-white

SMILES

CC1=C(C=CC=C1C2=CC=CC=C2)COC3=CC=C(C=C3Br)CN4CCCCC4C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (168.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0226 mL	10.1129 mL	20.2257 mL
5 mM	0.4045 mL	2.0226 mL	4.0451 mL
10 mM	0.2023 mL	1.0113 mL	2.0226 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (247 KB) HNMR (294 KB)

Handling Instructions (2659 KB)

References

[1]. Eun-Hye Kim, et al. Preparation of Biphenyl-Conjugated Bromotyrosine for Inhibition of PD-1/PD-L1 Immune Checkpoint Interactions. Int J Mol Sci. 2020 May 21;21(10):3639. [Content Brief]

[2]. Danfeng Shi, et al. Computational Insight Into the Small Molecule Intervening PD-L1 Dimerization and the Potential Structure-Activity Relationship. Front Chem. 2019 Nov 12;7:764. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0226 mL	10.1129 mL	20.2257 mL	50.5643 mL
	5 mM	0.4045 mL	2.0226 mL	4.0451 mL	10.1129 mL
	10 mM	0.2023 mL	1.0113 mL	2.0226 mL	5.0564 mL
	15 mM	0.1348 mL	0.6742 mL	1.3484 mL	3.3710 mL
	20 mM	0.1011 mL	0.5056 mL	1.0113 mL	2.5282 mL
	25 mM	0.0809 mL	0.4045 mL	0.8090 mL	2.0226 mL
	30 mM	0.0674 mL	0.3371 mL	0.6742 mL	1.6855 mL
	40 mM	0.0506 mL	0.2528 mL	0.5056 mL	1.2641 mL
	50 mM	0.0405 mL	0.2023 mL	0.4045 mL	1.0113 mL
	60 mM	0.0337 mL	0.1685 mL	0.3371 mL	0.8427 mL
	80 mM	0.0253 mL	0.1264 mL	0.2528 mL	0.6321 mL
	100 mM	0.0202 mL	0.1011 mL	0.2023 mL	0.5056 mL

BMS-8 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BMS-81675201-90-7BMS8BMS 8PD-1/PD-L1PD-1/Programmed death-ligand 1PD-1PD-L1PPI,Inhibitorinhibitorinhibit

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BMS-8

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