2. MAPK/ERK Pathway
  3. p38 MAPK
  4. Carbimazole

Carbimazole is an orally active antithyroid agent which rapidly converts to Methimazole after absorption and prevents thyroid peroxidase enzyme from iodinating and coupling the tyrosine residues on thyroglobulin, hence reducing the production of thyroxine. Carbimazole also displays anti-inflammatory and neuronal-protective activities, suggesting its application for hyperthyroidism and neurological research.

For research use only. We do not sell to patients.

Carbimazole Chemical Structure

Carbimazole Chemical Structure

CAS No. : 22232-54-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Other Forms of Carbimazole:

Carbimazole Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Carbimazole is an orally active antithyroid agent which rapidly converts to Methimazole after absorption and prevents thyroid peroxidase enzyme from iodinating and coupling the tyrosine residues on thyroglobulin, hence reducing the production of thyroxine. Carbimazole also displays anti-inflammatory and neuronal-protective activities, suggesting its application for hyperthyroidism and neurological research[1][2][3].

In Vitro

Carbimazole (0.01-2 mM, 6 h) inhibits the global translation of protein in SK-N-SH cells[1].
Carbimazole (0.5 mM, 12-72 h) inhibits hypoxic SK-N-SH cell injury and preserve intracellular ATP content in an oxygen-free atmosphere through inhibiting protein synthesis and activating MAPK[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Carbimazole Related Antibodies

Western Blot Analysis [1]

Cell Line: SK-N-SH
Concentration: 0.01-2 mM
Incubation Time: 6 h
Result: Induced the phosphorylation of eEF2 at Thr56 in a dose- and time-dependent manner through the phosphorylation of AMPK, resulting in a global translational inhibition.
In Vivo

Carbimazole (10, 15, 20 mg/animal/day, p.o., 21 d) shows mainly dose-dependent responses of basic physiological parameters in rabbits[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: local breed adult healthy male rabbits
Dosage: 10, 15, 20 mg/animal/day
Administration: Oral gavage (p.o.) for 21 d
Result: Decreased rectal temperature, respiration rate, heart rate, the packed cell volume, Hb concentration and total leukocyte count, significantly decreased plasma glucose level;
Increased serum levels of total protein, globulins while serum albumin level decreased;
Lowered serum urea level while there was an increase in serum urea level with increase in dose level;
Increased serum cholesterol level in a dose-dependent manner.
Clinical Trial
Molecular Weight

186.23

Formula

C7H10N2O2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1C=CN(C)C1=S)OCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (536.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.3697 mL 26.8485 mL 53.6970 mL
5 mM 1.0739 mL 5.3697 mL 10.7394 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (13.42 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (13.42 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.3697 mL 26.8485 mL 53.6970 mL 134.2426 mL
5 mM 1.0739 mL 5.3697 mL 10.7394 mL 26.8485 mL
10 mM 0.5370 mL 2.6849 mL 5.3697 mL 13.4243 mL
15 mM 0.3580 mL 1.7899 mL 3.5798 mL 8.9495 mL
20 mM 0.2685 mL 1.3424 mL 2.6849 mL 6.7121 mL
25 mM 0.2148 mL 1.0739 mL 2.1479 mL 5.3697 mL
30 mM 0.1790 mL 0.8950 mL 1.7899 mL 4.4748 mL
40 mM 0.1342 mL 0.6712 mL 1.3424 mL 3.3561 mL
50 mM 0.1074 mL 0.5370 mL 1.0739 mL 2.6849 mL
60 mM 0.0895 mL 0.4475 mL 0.8950 mL 2.2374 mL
80 mM 0.0671 mL 0.3356 mL 0.6712 mL 1.6780 mL
100 mM 0.0537 mL 0.2685 mL 0.5370 mL 1.3424 mL
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Carbimazole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Carbimazole22232-54-8p38 MAPKantithyroid agentGraves' diseaseMMImethimazoleSK-N-SHhyperthyroidismanti-inflammatoryneuronal-protectiveInhibitorinhibitorinhibit

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