Choline bitartrate

Name: Choline bitartrate
Brand: MedChemExpress
SKU: HY-101036
Rating: 5.0 (2 reviews)

Cat. No.: HY-101036 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Choline bitartrate is a vitamin-like essential nutrient, can affect diseases such as liver disease, atherosclerosis and neurological disorders. Choline bitartrate is a precursor for the neurotransmitter acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism.

For research use only. We do not sell to patients.

Choline bitartrate Chemical Structure

CAS No. : 87-67-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Choline bitartrate:

Choline chloride In-stock
Choline Get quote
Glycerophosphoinositol choline Get quote
Choline theophyllinate Get quote
Choline iodide Get quote
Choline tosylate Get quote
Choline bromide Get quote
Tricholine citrate Get quote
Choline hydroxide, 46 wt.% in water Get quote
Choline bitartrate (Standard) Get quote

2 Publications Citing Use of MCE Choline bitartrate

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mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

Biological Activity
Purity & Documentation
References
Customer Review

Description

Choline bitartrate is a vitamin-like essential nutrient, can affect diseases such as liver disease, atherosclerosis and neurological disorders^[1]^[2]. Choline bitartrate is a precursor for the neurotransmitter acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism^[3].

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Choline serves as a precursor component to two major cell membrane components: phosphatidylcholine and sphingomyelin in vitro^[3].
Choline is also a major source for methyl groups via its metabolite, betaine, which participates in S-adenosylmethionine synthesis pathways^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Choline bitartrate (oral gavage; 300 mg/kg vs. 5 g/kg) is administrated. Choline restriction (300 mg/kg) provokes robust ketosis and weight loss in mice, also causes significant hepatic steatosis, inflammation, and cellular injury. when Choline is repleted, moderate ketosis and hepatic fat accumulation can be attenuated^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

253.25

Formula

C₉H₁₉NO₇

CAS No.

87-67-2

Appearance

Solid

Color

White to off-white

SMILES

OCC[N+](C)(C)C.O=C(O)[C@H](O)[C@@H](O)C([O-])=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Animals

human milk

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 120 mg/mL (473.84 mM; Need ultrasonic)

DMSO : 55 mg/mL (217.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9487 mL	19.7433 mL	39.4867 mL
5 mM	0.7897 mL	3.9487 mL	7.8973 mL
10 mM	0.3949 mL	1.9743 mL	3.9487 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (10.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (10.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.75 mg/mL (10.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (394.87 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (256 KB) HNMR (257 KB)

Handling Instructions (2659 KB)

References

[1]. Hollenbeck CB, et al. An introduction to the nutrition and metabolism of choline. Cent Nerv Syst Agents Med Chem. 2012 Jun;12(2):100-13. [Content Brief]

[2]. Corbin KD, et al. Choline metabolism provides novel insights into nonalcoholic fatty liver disease and its progression. Curr Opin Gastroenterol. 2012 Mar;28(2):159-65. [Content Brief]

[3]. Shinobu Kuwae, et al. Development of a chemically defined platform fed-batch culture media for monoclonal antibody-producing CHO cell lines with optimized choline content. Cytotechnology. 2018 Jun;70(3):939-948. [Content Brief]

[4]. Rebecca C Schugar, et al. Role of choline deficiency in the Fatty liver phenotype of mice fed a low protein, very low carbohydrate ketogenic diet. PLoS One. 2013 Aug 29;8(8):e74806. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.9487 mL	19.7433 mL	39.4867 mL	98.7167 mL
	5 mM	0.7897 mL	3.9487 mL	7.8973 mL	19.7433 mL
	10 mM	0.3949 mL	1.9743 mL	3.9487 mL	9.8717 mL
	15 mM	0.2632 mL	1.3162 mL	2.6324 mL	6.5811 mL
	20 mM	0.1974 mL	0.9872 mL	1.9743 mL	4.9358 mL
	25 mM	0.1579 mL	0.7897 mL	1.5795 mL	3.9487 mL
	30 mM	0.1316 mL	0.6581 mL	1.3162 mL	3.2906 mL
	40 mM	0.0987 mL	0.4936 mL	0.9872 mL	2.4679 mL
	50 mM	0.0790 mL	0.3949 mL	0.7897 mL	1.9743 mL
	60 mM	0.0658 mL	0.3291 mL	0.6581 mL	1.6453 mL
	80 mM	0.0494 mL	0.2468 mL	0.4936 mL	1.2340 mL
	100 mM	0.0395 mL	0.1974 mL	0.3949 mL	0.9872 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.