2. Neuronal Signaling Membrane Transporter/Ion Channel Metabolic Enzyme/Protease
  3. nAChR Endogenous Metabolite
  4. Choline chloride

Choline chloride is an essential nutrient that activates alpha7 nicotinic receptors and has analgesic and anti-inflammatory activity. Glycerophosphoinositol choline can affect diseases such as liver disease, atherosclerosis and neurological disorders.

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Choline chloride Chemical Structure

Choline chloride Chemical Structure

CAS No. : 67-48-1

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Choline chloride:

Choline chloride Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Choline chloride is an essential nutrient that activates alpha7 nicotinic receptors and has analgesic and anti-inflammatory activity. Glycerophosphoinositol choline can affect diseases such as liver disease, atherosclerosis and neurological disorders[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Choline chloride (0 or 70 μM, 4 days) can effectively mitigate apoptosis and maintain cell viability[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Choline chloride Related Antibodies

Cell Viability Assay[1]

Cell Line: Rat pheochromocytoma cells PC12
Concentration: 0 or 70 μM
Incubation Time: 4 days
Result: Showed the cell viability of 94% at 70 μM while cell viability of 83% at 0 μM.
Reduced the number of cells with DNA breaks (characteristic of apoptosis) by 8.5% at 70 μM compared to the no treatment group.
In Vivo

Choline chloride (s.c., 0.2 and 100 mg/kg/h, 24 or 48 hours) can reduce postoperative injurious reflexes and effectively decreases tumor necrosis factor (TNF) release from macrophages in female C57/Bl6 mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Postoperative pain model of female C57/Bl6 mice[2]
Dosage: 0.2 and 100 mg/kg/h
Administration: Subcutaneous injection, 24 or 48 hours
Result: Reduced heat hypersensitivity after surgery with maximal efficacy after 48 h treatment and the ED50 value of choline dose was 1.7 mg/kg/h.
Reduced hypersensitivity to punctate mechanical stimuli 48 hours after infusion in a dose-dependent manner and with the ED50 value of 4.7 mg/kg/h but not 24 hours.
Clinical Trial
Molecular Weight

139.62

Formula

C5H14ClNO
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OCC[N+](C)(C)C.[Cl-]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature, keep dry and cool
Solvent & Solubility
In Vitro: 

DMSO : ≥ 140 mg/mL (1002.72 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 100 mg/mL (716.23 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.1623 mL 35.8115 mL 71.6230 mL
5 mM 1.4325 mL 7.1623 mL 14.3246 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.5 mg/mL (25.07 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 130 mg/mL (931.10 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (244 KB) HNMR (174 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 7.1623 mL 35.8115 mL 71.6230 mL 179.0574 mL
5 mM 1.4325 mL 7.1623 mL 14.3246 mL 35.8115 mL
10 mM 0.7162 mL 3.5811 mL 7.1623 mL 17.9057 mL
15 mM 0.4775 mL 2.3874 mL 4.7749 mL 11.9372 mL
20 mM 0.3581 mL 1.7906 mL 3.5811 mL 8.9529 mL
25 mM 0.2865 mL 1.4325 mL 2.8649 mL 7.1623 mL
30 mM 0.2387 mL 1.1937 mL 2.3874 mL 5.9686 mL
40 mM 0.1791 mL 0.8953 mL 1.7906 mL 4.4764 mL
50 mM 0.1432 mL 0.7162 mL 1.4325 mL 3.5811 mL
60 mM 0.1194 mL 0.5969 mL 1.1937 mL 2.9843 mL
80 mM 0.0895 mL 0.4476 mL 0.8953 mL 2.2382 mL
100 mM 0.0716 mL 0.3581 mL 0.7162 mL 1.7906 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Choline chloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Choline67-48-1nAChREndogenous MetaboliteNicotinic acetylcholine receptorscholinealpha7 nicotinic receptoranalgesicanti-inflammatoryInhibitorinhibitorinhibit

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