cis-4-Hydroxy-L-proline

Name: cis-4-Hydroxy-L-proline
Brand: MedChemExpress
SKU: HY-40136
Rating: 4.5 (1 reviews)

Cat. No.: HY-40136 Purity: ≥98.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

cis-4-Hydroxy-L-proline inhibits cell growth by preventing the deposition of triple helical collagen on the cell layer. cis-4-Hydroxy-L-proline inhibits N-nitrosomethylurea-induced breast tumor growth. cis-4-Hydroxy-L-proline improves lung compliance and reduces prostate weight. cis-4-Hydroxy-L-proline can be used in the study of hypoxic pulmonary hypertension.

For research use only. We do not sell to patients.

cis-4-Hydroxy-L-proline Chemical Structure

CAS No. : 618-27-9

Size	Stock	Quantity
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of cis-4-Hydroxy-L-proline:

cis-4-Hydroxy-L-proline-d₃ Get quote
cis-4-Hydroxy-L-proline hydrochloride Get quote
cis-4-Hydroxy-L-proline (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

cis-4-Hydroxy-L-proline (100-200 μg/mL; 1 week) inhibits the accumulation of collagenous and noncollagenous proteins in the culture medium in the in vitro co-culture experiment of rat Schwann cells (SC) and dorsal root ganglion neurons^[1].
cis-4-Hydroxy-L-proline (200 μg/mL; 6 h) reduces the proportion of collagen synthesis in total protein synthesis and increases the degradation proportion of newly synthesized collagen in freshly isolated chick tendon fibroblasts^[2].
cis-4-Hydroxy-L-proline (3200-32000 μg/ml; 24 h) significantly reduces the production of albumin and urea by hepatocytes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

cis-4-Hydroxy-L-proline (285-903 mg/kg; p.o.; 28 days) causes signs of liver damage in rats, showing elevated alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT)^[3].
cis-4-Hydroxy-L-proline (200 mg/kg; s.c.; twice daily; 30 days) partially prevents the accumulation of collagen in the lungs and partially improves the decrease in lung compliance in the Bleomycin (HY-108345)-induced pulmonary fibrosis model experiment of hamsters^[4].
cis-4-Hydroxy-L-proline (200 mg/kg; s.c.; injected alone for 3 days first and then co-injected with dihydrotestosterone for 10 days) significantly reduces the wet weight of the prostate, inhibits the proliferation and differentiation of prostatic glandular cells in response to androgen stimulation in Androgen-induced prostate growth model in prepubertal castrated guinea pigs^[5].
CHOP-PEG (polymeric prodrug of cis-4-Hydroxy-L-proline, 0.8 mg by continuous s.c.; 10 mg by single s.c.; 40 mg by single i.v.; 90 mg by single intratracheal injection; 7 days) can prevent and reverse chronic hypoxic pulmonary hypertension in the rat hypoxic pulmonary hypertension model^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rats^[3]
Dosage:	285 mg/kg, 507 mg/kg, 903 mg/kg
Administration:	Oral gavage (p.o.), 28 days
Result:	Increased ALAT and ASAT at a dose of 903 mg/kg. Increased albumin at 507 mg/kg. Increased blood urea nitrogen at 903 mg/kg CHP. Revealed a pale appearance of the liver due to drug-induced anemia.

Molecular Weight

131.13

Formula

C₅H₉NO₃

CAS No.

618-27-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@H](C1)NC[C@H]1O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (762.60 mM; Need ultrasonic)

DMSO : 1 mg/mL (7.63 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.6260 mL	38.1301 mL	76.2602 mL
5 mM	1.5252 mL	7.6260 mL	15.2520 mL
10 mM	0.7626 mL	3.8130 mL	7.6260 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (762.60 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (251 KB) HNMR (115 KB) OR (83 KB)

Handling Instructions (2659 KB)

References

[1]. Eldridge CF, et al. Effects of cis-4-hydroxy-L-proline, and inhibitor of Schwann cell differentiation, on the secretion of collagenous and noncollagenous proteins by Schwann cells. Exp Cell Res. 1988 Feb;174(2):491-501. [Content Brief]

[2]. Neblock DS, et al. The effect of cis-4-hydroxy-L-proline on intracellular degradation of newly synthesized collagen by freshly isolated chick tendon fibroblasts. Connect Tissue Res. 1982;10(3-4):297-301. [Content Brief]

[3]. Dickens H, et al. Anticancer drug cis-4-hydroxy-L-proline: Correlation of preclinical toxicology with clinical parameters of liver function. Mol Med Rep. 2008 Jul-Aug;1(4):459-64. [Content Brief]

[4]. Riley DJ, et al. Effect of cis-4-hydroxy-L-proline on lung mechanics and collagen content of bleomycin-treated hamsters. Chest. 1981 Jul;80(1 Suppl):49-50. [Content Brief]

[5]. Wong YC, et al. Effects of cis-4-hydroxy-L-proline on the androgen-induced growth of the lateral prostate of the prepubertally castrated guinea pig. Prostate. 1993;23(4):337-54. [Content Brief]

[6]. Simon PM, et al. Prodrug of proline analogue reduces hypoxic pulmonary hypertension in rats. Pulm Pharmacol Ther. 2006;19(4):242-50. [Content Brief]

[7]. Tan EM, et, al. Proline analogues inhibit human skin fibroblast growth and collagen production in culture. J Invest Dermatol. 1983 Apr;80(4):261-7. [Content Brief]

[8]. Riley DJ, et, al. Prevention of bleomycin-induced pulmonary fibrosis in the hamster by cis-4-hydroxy-l-proline. Am Rev Respir Dis. 1981 Apr;123(4 Pt 1):388-93. [Content Brief]

[9]. Lewko WM, et, al. Sensitivity of N-nitrosomethylurea-induced rat mammary tumors to cis-hydroxyproline, an inhibitor of collagen production. Cancer Res. 1981 Jul;41(7):2855-62. [Content Brief]

[10]. Kao WW, et, al. Proline analogue removes fibroblasts from cultured mixed cell populations. Nature. 1977 Mar 3;266(5597):63-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	7.6260 mL	38.1301 mL	76.2602 mL	190.6505 mL
DMSO / H₂O	5 mM	1.5252 mL	7.6260 mL	15.2520 mL	38.1301 mL
H₂O	10 mM	0.7626 mL	3.8130 mL	7.6260 mL	19.0650 mL
	15 mM	0.5084 mL	2.5420 mL	5.0840 mL	12.7100 mL
	20 mM	0.3813 mL	1.9065 mL	3.8130 mL	9.5325 mL
	25 mM	0.3050 mL	1.5252 mL	3.0504 mL	7.6260 mL
	30 mM	0.2542 mL	1.2710 mL	2.5420 mL	6.3550 mL
	40 mM	0.1907 mL	0.9533 mL	1.9065 mL	4.7663 mL
	50 mM	0.1525 mL	0.7626 mL	1.5252 mL	3.8130 mL
	60 mM	0.1271 mL	0.6355 mL	1.2710 mL	3.1775 mL
	80 mM	0.0953 mL	0.4766 mL	0.9533 mL	2.3831 mL
	100 mM	0.0763 mL	0.3813 mL	0.7626 mL	1.9065 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.