2. MAPK/ERK Pathway Stem Cell/Wnt
  3. JNK p38 MAPK ERK
  4. Citropten

Citropten  (Synonyms: 5,7-Dimethoxycoumarin; Limettin)

Cat. No.: HY-N7085 Purity: 99.28%
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Citropten (5, 7-dimethoxycoumarin) is one of the coumarin derivatives. Citropten is an effective oral anticancer agent. Citropten has anti-proliferative activity against A2058 and B16 melanoma cells. Citropten exerts anti-inflammatory effects through the NFκB and MAPK signaling pathways. Citropten acts as an antidepressant through heat shock protein-70, monoamine oxidase-A and inhibition of apoptosis .

For research use only. We do not sell to patients.

Citropten Chemical Structure

Citropten Chemical Structure

CAS No. : 487-06-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 In-stock
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10 mM * 1 mL in DMSO USD 33 In-stock
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50 mg USD 30 In-stock
100 mg USD 50 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Citropten:

Citropten Related Antibodies

Top Publications Citing Use of Products

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JNK1 JNK2 JNK3 JNK

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

Citropten (5, 7-dimethoxycoumarin) is one of the coumarin derivatives. Citropten is an effective oral anticancer agent. Citropten has anti-proliferative activity against A2058 and B16 melanoma cells. Citropten exerts anti-inflammatory effects through the NFκB and MAPK signaling pathways. Citropten acts as an antidepressant through heat shock protein-70, monoamine oxidase-A and inhibition of apoptosis[1] [2] [3].

In Vitro

Citropten (10-500 mM; 72 h) inhibits the proliferation in A2058 human melanoma cell line[1].
Citropten (0-40 μM; 24 h) reduces NFκB and MAPK signaling pathway in activated Jurkat and HT-29 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Citropten Related Antibodies

Cell Proliferation Assay[1]

Cell Line: A2058 human melanoma cell
Concentration: 10, 50, 100, 150 and 500 mM
Incubation Time: 72 h
Result: Inhibited cell proliferation in a concentration‐dependent manner.

Cell Proliferation Assay[2]

Cell Line: Activated Jurkat and HT-29 cells
Concentration: 0, 10, 20 and 40 μM
Incubation Time: 24 h
Result: Inhibited the phosphorylation levels of IκBα, ERK, p38 and JNK.
In Vivo

Citropten (10 mg/kg; Oral administration; Every day; 7 days) has anti-inflammatory effects and modulates T cell and intestinal epithelial cell activity in mouse models of colitis induced by DSS (HY-116282)[2].
Citropten (10 mg/kg; Intraperitoneal injection; Single injection) exerts an antidepressant effect by increasing the level of heat shock protein-70 and inhibiting the level of monoamine oxidase-A in rat models of chronic mild stress depression[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DSS (HY-116282) treated C57BL/6J female mice aged 6-8 weeks old[2]
Dosage: 10 mg/kg
Administration: Oral administration (p.o.); every day; 7 days
Result: Ameliorated the severity of inflammation on colonic tissues.
Reduced cytokine (TNFα, IL-1β and IL-8) production on colonic tissues.
Attenuated the activity of effector T cells.
Animal Model: Chronic mild stress treated male adult Sprague-Dawley rats (around 190 g)[3]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection (i.p.), Single injection
Result: Prevented the chronic mild stress induced damage to locomotor activity.
Inhibited chronic mild stress induced increase in level of monoamine oxidase-A.
Inhibited the chronic mild stress induced increase in level ofcaspase-3.
Increased the level of heat shock protein-70 in the rat hippocampus.
Molecular Weight

206.20

Formula

C11H10O4
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1OC2=C(C(OC)=CC(OC)=C2)C=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (121.24 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8497 mL 24.2487 mL 48.4973 mL
5 mM 0.9699 mL 4.8497 mL 9.6995 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (10.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (10.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

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Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.41%

SDS (393 KB)

COA (249 KB) HNMR (241 KB) LCMS (269 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.8497 mL 24.2487 mL 48.4973 mL 121.2433 mL
5 mM 0.9699 mL 4.8497 mL 9.6995 mL 24.2487 mL
10 mM 0.4850 mL 2.4249 mL 4.8497 mL 12.1243 mL
15 mM 0.3233 mL 1.6166 mL 3.2332 mL 8.0829 mL
20 mM 0.2425 mL 1.2124 mL 2.4249 mL 6.0622 mL
25 mM 0.1940 mL 0.9699 mL 1.9399 mL 4.8497 mL
30 mM 0.1617 mL 0.8083 mL 1.6166 mL 4.0414 mL
40 mM 0.1212 mL 0.6062 mL 1.2124 mL 3.0311 mL
50 mM 0.0970 mL 0.4850 mL 0.9699 mL 2.4249 mL
60 mM 0.0808 mL 0.4041 mL 0.8083 mL 2.0207 mL
80 mM 0.0606 mL 0.3031 mL 0.6062 mL 1.5155 mL
100 mM 0.0485 mL 0.2425 mL 0.4850 mL 1.2124 mL
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Citropten Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Citropten487-06-95,7-Dimethoxycoumarin LimettinJNKp38 MAPKERKc-Jun N-terminal kinaseExtracellular signal regulated kinasesA2058 and B16 melanoma cellsColitis modelDepressive disorderJurkat cellHT-29cellInhibitorinhibitorinhibit

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