2. NF-κB Apoptosis
  3. Keap1-Nrf2 Apoptosis
  4. Coniferaldehyde

Coniferaldehyde  (Synonyms: 4-Hydroxy-3-methoxycinnamaldehyde)

Cat. No.: HY-N2535 Purity: 99.94%
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Coniferaldehyde (4-Hydroxy-3-methoxycinnamaldehyde) is an effective inducer of heme oxygenase-1 (HO-1). Coniferaldehyde inhibits LPS-induced apoptosis through the PKCα/β II/Nrf-2/HO-1 dependent pathway in RAW264.7 macrophage cells. Coniferaldehyde has antioxidant and anti-inflammatory activities.

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Coniferaldehyde Chemical Structure

Coniferaldehyde Chemical Structure

CAS No. : 458-36-6

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Coniferaldehyde:

Coniferaldehyde Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Coniferaldehyde

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Coniferaldehyde (4-Hydroxy-3-methoxycinnamaldehyde) is an effective inducer of heme oxygenase-1 (HO-1). Coniferaldehyde inhibits LPS-induced apoptosis through the PKCα/β II/Nrf-2/HO-1 dependent pathway in RAW264.7 macrophage cells. Coniferaldehyde has antioxidant and anti-inflammatory activities[1].

In Vitro

Coniferaldehyde (0.1-5 μM; Pretreatment with 1 h; and then treated with 24 h) exerts cytoprotective function against LPS-induced cell death and dramatically inhibits LPS-induced NO production as assessed by the Griess reaction in a dose dependent manner[1].
Coniferaldehyde (0.5-5 μM; 4-24 h) dramatically increases the Nrf-2 nuclear translocation and HO-1 expression. Furthermore, Coniferaldehyde specifically enhances the phosphorylation of PKCα/PKCβ II[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Coniferaldehyde Related Antibodies

Cell Viability Assay[1]

Cell Line: Raw264.7 cells inudced with LPS
Concentration: 0.1 μM, 0.5 μM, 1 μM, 2 μM, 5 μM
Incubation Time: Pretreatment with 1 h, and then treated with 24 h
Result: Inhibited LPS-induced NO production and cell death.

Western Blot Analysis[1]

Cell Line: Raw264.7 cells
Concentration: 0.5 μM, 1 μM, 2 μM, 5 μM
Incubation Time: 4 h, 8 h, 12 h, 24 h
Result: Increased HO-1 protein level in a dose- and time-dependent manner.
In Vivo

Coniferaldehyde (0.05 mmol kg/day; i.p; for 6 weeks) activates the Nrf2 signaling pathway in primary chondrocytes and articular cartilage from the knee joints. And Coniferaldehyde alleviates cartilage destruction in OA mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: B6 male mice (26 g; 8-10 weeks old), surgical-induced osteoarthritis (OA)[2]
Dosage: 0.05 mmol kg/day (approx. 8.9 mg/kg)
Administration: i.p; for 6 weeks
Result: Evidently alleviated the medial meniscus cartilage damage in mice subjected to the destabilization.
Molecular Weight

178.18

Formula

C10H10O3
CAS No.
Appearance

Solid

Color

Light yellow to brown

SMILES

O=C/C=C/C1=CC=C(O)C(OC)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (561.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.6123 mL 28.0615 mL 56.1230 mL
5 mM 1.1225 mL 5.6123 mL 11.2246 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (5.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1 mg/mL (5.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.94%

SDS (393 KB)

COA (245 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.6123 mL 28.0615 mL 56.1230 mL 140.3076 mL
5 mM 1.1225 mL 5.6123 mL 11.2246 mL 28.0615 mL
10 mM 0.5612 mL 2.8062 mL 5.6123 mL 14.0308 mL
15 mM 0.3742 mL 1.8708 mL 3.7415 mL 9.3538 mL
20 mM 0.2806 mL 1.4031 mL 2.8062 mL 7.0154 mL
25 mM 0.2245 mL 1.1225 mL 2.2449 mL 5.6123 mL
30 mM 0.1871 mL 0.9354 mL 1.8708 mL 4.6769 mL
40 mM 0.1403 mL 0.7015 mL 1.4031 mL 3.5077 mL
50 mM 0.1122 mL 0.5612 mL 1.1225 mL 2.8062 mL
60 mM 0.0935 mL 0.4677 mL 0.9354 mL 2.3385 mL
80 mM 0.0702 mL 0.3508 mL 0.7015 mL 1.7538 mL
100 mM 0.0561 mL 0.2806 mL 0.5612 mL 1.4031 mL
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Coniferaldehyde Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coniferaldehyde458-36-64-Hydroxy-3-methoxycinnamaldehydeKeap1-Nrf2ApoptosisInhibitorinhibitorinhibit

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