2. Metabolic Enzyme/Protease
  3. Mitochondrial Metabolism
  4. Diphenyl Phosphate

Diphenyl Phosphate inhibits growth and energy metabolism in zebrafish and mice in a sex-specific manner. Diphenyl Phosphate can inhibit the activity of SDH (respiratory complex II), reduce the expression of CPT1 and disrupts the integrity of the mitochondrial membrane. Diphenyl Phosphate may be used in research on metabolic diseases.

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Diphenyl Phosphate Chemical Structure

Diphenyl Phosphate Chemical Structure

CAS No. : 838-85-7

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Other Forms of Diphenyl Phosphate:

Diphenyl Phosphate Related Antibodies

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Description

Diphenyl Phosphate inhibits growth and energy metabolism in zebrafish and mice in a sex-specific manner. Diphenyl Phosphate can inhibit the activity of SDH (respiratory complex II), reduce the expression of CPT1 and disrupts the integrity of the mitochondrial membrane. Diphenyl Phosphate may be used in research on metabolic diseases[1][2].

IC50 & Target

Succinate Dehydrogenase (SDH); Carnitine Palmitoyltransferase 1 (CPT1)
In Vivo

Diphenyl Phosphate (2 or 200 mg/kg, s.c., once daily for 10 days) has no adverse effects on uterine weight and serum estradiol levels in ICR mice, but causes disturbances in lipid metabolism characteristics[2].
Chronic exposure to DPhP (0.8-35.6 μg/L, exposure for 120 days) can retard growth in male zebrafish by inhibiting energy output[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Primigravida ICR mice (days 1-10 post-birth)[2]
Dosage: 2 or 200 mg
Administration: Subcutaneous injection (s.c.), once daily for 10 days
Result: Exposure increased the body weight of male offspring and led to an increase in lipid, taurine, unsaturated fatty acids, polyunsaturated fatty acids, and phosphatidylcholine in male metabolic profiles at a low dose, while having no significant effect on female mice.
Animal Model: Zebrafish[1]
Dosage: 0.8, 3.9, 35.6 μg/L
Administration: Exposure (in the aquatic environment of zebrafish), 120 days
Result: Caused significant decreases in body mass and body length of male zebrafish (at the dose of 35.6 μg/L), whereas no significantly effects were observed for female zebrafish.
Molecular Weight

250.19

Formula

C12H11O4P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=P(OC1=CC=CC=C1)(OC2=CC=CC=C2)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (399.70 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9970 mL 19.9848 mL 39.9696 mL
5 mM 0.7994 mL 3.9970 mL 7.9939 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.96%

COA (248 KB) HNMR (124 KB) RP-HPLC (258 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.9970 mL 19.9848 mL 39.9696 mL 99.9241 mL
5 mM 0.7994 mL 3.9970 mL 7.9939 mL 19.9848 mL
10 mM 0.3997 mL 1.9985 mL 3.9970 mL 9.9924 mL
15 mM 0.2665 mL 1.3323 mL 2.6646 mL 6.6616 mL
20 mM 0.1998 mL 0.9992 mL 1.9985 mL 4.9962 mL
25 mM 0.1599 mL 0.7994 mL 1.5988 mL 3.9970 mL
30 mM 0.1332 mL 0.6662 mL 1.3323 mL 3.3308 mL
40 mM 0.0999 mL 0.4996 mL 0.9992 mL 2.4981 mL
50 mM 0.0799 mL 0.3997 mL 0.7994 mL 1.9985 mL
60 mM 0.0666 mL 0.3331 mL 0.6662 mL 1.6654 mL
80 mM 0.0500 mL 0.2498 mL 0.4996 mL 1.2491 mL
100 mM 0.0400 mL 0.1998 mL 0.3997 mL 0.9992 mL
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Diphenyl Phosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Diphenyl838-85-7Mitochondrial MetabolismICR miceGrowth inhibitoryMetabolic diseasesInhibitorinhibitorinhibit

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