DMPO

Cat. No.: HY-107690 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

DMPO is a cell permeable hydrophillic spin trap agent for superoxide detection.

For research use only. We do not sell to patients.

DMPO Chemical Structure

CAS No. : 3317-61-1

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

DMPO is a cell permeable hydrophillic spin trap agent for superoxide detection^[1].

In Vitro

DMPO (100 μM, 24 h) attenuates SIN-1 (3-Morpholinosydnonimine (HY-126849)) (500 μM, 2 h)-mediated cytotoxicity and ROS generation in BAEC or HEK293 cells^[3].
DMPO (100 μM, 24 h) increases NO levels via increasing eNOS activity and phospho-eNOS levels in BAEC cells^[3].
DMPO (1-100 mM) inhibits FMLP and concanavalin A (HY-P2149) induced ·O₂^- secretion in neutrophils^[4].
DMPO (50 mM, 24 h) inhibits Lipopolysaccharides (HY-D1056)-triggered M1-linked pro-inflammatory cytokine (IL-1β, IL-6 and TNF-α) and NO production in RAW 264,6 cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	BAEC cells
Concentration:	100 μM
Incubation Time:	12 and 24 h
Result:	Increased p-eNOS and p-Akt level.

In Vivo

DMPO (10-100 mg/kg, i.p.) shows antinociceptive effect in rats subjected to Formalin induced hyperalgesia^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

113.16

Formula

C₆H₁₁NO

CAS No.

3317-61-1

Appearance

<25°C Solid,>29°C Liquid

Color

Colorless to light yellow

SMILES

CC1(C)[N+]([O-])=CCC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (883.70 mM)

DMSO : 100 mg/mL (883.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.8370 mL	44.1852 mL	88.3704 mL
5 mM	1.7674 mL	8.8370 mL	17.6741 mL
10 mM	0.8837 mL	4.4185 mL	8.8370 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (22.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (22.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (256 KB) HNMR (218 KB) RP-HPLC (261 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Ranguelova K, Mason RP. The fidelity of spin trapping with DMPO in biological systems. Magn Reson Chem. 2011;49(4):152-158. [Content Brief]

[2]. Konaka R, Kawai M, Noda H, Kohno M, Niwa R. Synthesis and evaluation of DMPO-type spin traps. Free Radic Res. 1995;23(1):15-25. [Content Brief]

[3]. Das A, et al. Reversal of SIN-1-induced eNOS dysfunction by the spin trap, DMPO, in bovine aortic endothelial cells via eNOS phosphorylation. Br J Pharmacol. 2014 May;171(9):2321-34. [Content Brief]

[4]. Britigan BE, Hamill DR. Effect of the spin trap 5,5 dimethyl-1-pyrroline-N-oxide (DMPO) on human neutrophil function: novel inhibition of neutrophil stimulus-response coupling? Free Radic Biol Med. 1990;8(5):459-70. [Content Brief]

[5]. Zhai Z, et al. The spin trap 5,5-dimethyl-1-pyrroline N-oxide inhibits lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Life Sci. 2012 Mar 10;90(11-12):432-9. [Content Brief]

[6]. Woo suk Chung, et al. Antinociceptive Effect of Intraperitoneally Administered 5,5-dimethyl-1-pyrroline N-oxide on Formalin Induced Nociception in Rats. Korean Journal of Anesthesiology 2008;54(3):S35-S39.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.8370 mL	44.1852 mL	88.3704 mL	220.9261 mL
	5 mM	1.7674 mL	8.8370 mL	17.6741 mL	44.1852 mL
	10 mM	0.8837 mL	4.4185 mL	8.8370 mL	22.0926 mL
	15 mM	0.5891 mL	2.9457 mL	5.8914 mL	14.7284 mL
	20 mM	0.4419 mL	2.2093 mL	4.4185 mL	11.0463 mL
	25 mM	0.3535 mL	1.7674 mL	3.5348 mL	8.8370 mL
	30 mM	0.2946 mL	1.4728 mL	2.9457 mL	7.3642 mL
	40 mM	0.2209 mL	1.1046 mL	2.2093 mL	5.5232 mL
	50 mM	0.1767 mL	0.8837 mL	1.7674 mL	4.4185 mL
	60 mM	0.1473 mL	0.7364 mL	1.4728 mL	3.6821 mL
	80 mM	0.1105 mL	0.5523 mL	1.1046 mL	2.7616 mL
	100 mM	0.0884 mL	0.4419 mL	0.8837 mL	2.2093 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

DMPO Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DMPO3317-61-1Reactive Oxygen SpeciesNO SynthaseNitric oxide synthasesNOSantinociceptiveeNOSROSBAEC cellInhibitorinhibitorinhibit

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