VH032-cyclopropane-F (Synonyms: VHL ligand 3; E3 ligase Ligand 19)

Name: VH032-cyclopropane-F
Brand: MedChemExpress
SKU: HY-125905
Rating: 4.5 (1 reviews)

Cat. No.: HY-125905 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

VH032-cyclopropane-F is the VH032-based VHL ligand. VH032-cyclopropane-F can be connected to the ligand for protein (e.g., SMARCA BD ligand) by a linker to form PROTACs (e.g., PROTAC 1). PROTAC 1 is a partial degrader of SMARCA2 and SMARCA4.

For research use only. We do not sell to patients.

VH032-cyclopropane-F Chemical Structure

CAS No. : 2306193-99-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 253	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 253	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 230	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 640	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE VH032-cyclopropane-F

•Exp Cell Res. 12 October 2021, 112868.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Ligands for E3 Ligase Isoform Specific Products:

View All Isoforms

von Hippel-Lindau (VHL) MDM2 Cereblon IAP KLHDC2 Fbxo3 RNF4 DCAF11 DCAF1 UHRF1

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

VHL

In Vitro

VH032-cyclopropane-F (5 μM, 24 h) increases FAK expression levels in A427 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

532.63

Formula

C₂₆H₃₃FN₄O₅S

CAS No.

2306193-99-5

Appearance

Solid

Color

White to yellow

SMILES

O=C(NCC1=CC=C(C=C1O)C2=C(N=CS2)C)[C@H]3N(C[C@@H](C3)O)C([C@H](C(C)(C)C)NC(C4(CC4)F)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (187.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8775 mL	9.3874 mL	18.7748 mL
5 mM	0.3755 mL	1.8775 mL	3.7550 mL
10 mM	0.1877 mL	0.9387 mL	1.8775 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (260 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Farnaby W, et al. BAF complex vulnerabilities in cancer demonstrated via structure-based PROTAC design. Nat Chem Biol. 2019 Jul;15(7):672-680. [Content Brief]

[2]. Liu J, et al. FAK-targeting PROTAC demonstrates enhanced antitumor activity against KRAS mutant non-small cell lung cancer. Exp Cell Res. 2021 Nov 15;408(2):112868. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8775 mL	9.3874 mL	18.7748 mL	46.9369 mL
	5 mM	0.3755 mL	1.8775 mL	3.7550 mL	9.3874 mL
	10 mM	0.1877 mL	0.9387 mL	1.8775 mL	4.6937 mL
	15 mM	0.1252 mL	0.6258 mL	1.2517 mL	3.1291 mL
	20 mM	0.0939 mL	0.4694 mL	0.9387 mL	2.3468 mL
	25 mM	0.0751 mL	0.3755 mL	0.7510 mL	1.8775 mL
	30 mM	0.0626 mL	0.3129 mL	0.6258 mL	1.5646 mL
	40 mM	0.0469 mL	0.2347 mL	0.4694 mL	1.1734 mL
	50 mM	0.0375 mL	0.1877 mL	0.3755 mL	0.9387 mL
	60 mM	0.0313 mL	0.1565 mL	0.3129 mL	0.7823 mL
	80 mM	0.0235 mL	0.1173 mL	0.2347 mL	0.5867 mL
	100 mM	0.0188 mL	0.0939 mL	0.1877 mL	0.4694 mL

VH032-cyclopropane-F Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VH032-cyclopropane-F2306193-99-5VHL ligand 3 E3 ligase Ligand 19VHL ligand3VHL ligand-3Ligands for E3 LigaseE3 ligase-recruiting MoietyA427 cellsFAKVHL ligandlinkerPROTACSMARCA2SMARCA4Inhibitorinhibitorinhibit

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VH032-cyclopropane-F (Synonyms: VHL ligand 3; E3 ligase Ligand 19)

Customer Review

VH032-cyclopropane-F Related Antibodies

1 Publications Citing Use of MCE VH032-cyclopropane-F

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Ligands for E3 Ligase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

VH032-cyclopropane-F Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

VH032-cyclopropane-F Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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