2. Stem Cell/Wnt Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Wnt Reactive Oxygen Species
  4. Echinacoside

Echinacoside, one of the phenylethanoids isolated from the stems of Cistanche deserticola, effectively inhibits Wnt/β-catenin signaling. Echinacoside elicits neuroprotection by activating Trk receptors and their downstream signal pathways. Antiosteoporotic activity.

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Echinacoside Chemical Structure

Echinacoside Chemical Structure

CAS No. : 82854-37-3

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10 mM * 1 mL in DMSO
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50 mg USD 240 In-stock
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Echinacoside:

Echinacoside Related Antibodies

Top Publications Citing Use of Products

    Echinacoside purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2020 May 21;526(1):170-175.  [Abstract]

    Echinacoside inhibits the expression of key Wnt/b-catenin proteins in breast cancer cell lines. MDA-MB-231 and MDA-MB-468 cells are treated with the indicated concentrations of Echinacoside for 24 h. LEF1, CD44, and cyclin D1 protein expression levels are detected by western blot.

    Echinacoside purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2020 May 21;526(1):170-175.  [Abstract]

    Echinacoside inhibits the expression of key Wnt/b-catenin proteins in breast cancer cell lines. MDA-MB-231 and MDA-MB-468 cells are treated with the indicated concentrations of Echinacoside for 24 h. Cells are harvested, and proteins are extracted using RIPA cell lysis buffer.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Echinacoside, one of the phenylethanoids isolated from the stems of Cistanche deserticola, effectively inhibits Wnt/β-catenin signaling. Echinacoside elicits neuroprotection by activating Trk receptors and their downstream signal pathways. Antiosteoporotic activity[1][2][3].

    In Vivo

    Echinacoside (30-270 mg/kg body weight; p.o.; daily for 12 weeks) significantly reverses the increases of body weight, serum hydroxyproline (HOP) levels, and the decreases of uterus wet weight and bone mineral density (BMD) in In ovariectomized (OVX) rats[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Fifty-six aged 6 months female Sprague-Dawley rats (OVX rat model)[3]
    Dosage: 30, 90, 270 mg/kg body weight
    Administration: p.o.; daily for 12 weeks
    Result: The increases of body weight, serum hydroxyproline (HOP) levels, and the decreases of uterus wet weight and BMD were significantly reversed.
    Molecular Weight

    786.73

    Formula

    C35H46O20
    CAS No.
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    OC1=CC(/C=C/C(O[C@H]2[C@H](O[C@H]3[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O3)[C@@H](O)[C@H](OCCC4=CC(O)=C(O)C=C4)O[C@@H]2CO[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)=O)=CC=C1O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (63.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 35.71 mg/mL (45.39 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.2711 mL 6.3554 mL 12.7108 mL
    5 mM 0.2542 mL 1.2711 mL 2.5422 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.18 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.18 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (127.11 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.88%

    COA (255 KB) HNMR (304 KB) RP-HPLC (241 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.2711 mL 6.3554 mL 12.7108 mL 31.7771 mL
    5 mM 0.2542 mL 1.2711 mL 2.5422 mL 6.3554 mL
    10 mM 0.1271 mL 0.6355 mL 1.2711 mL 3.1777 mL
    15 mM 0.0847 mL 0.4237 mL 0.8474 mL 2.1185 mL
    20 mM 0.0636 mL 0.3178 mL 0.6355 mL 1.5889 mL
    25 mM 0.0508 mL 0.2542 mL 0.5084 mL 1.2711 mL
    30 mM 0.0424 mL 0.2118 mL 0.4237 mL 1.0592 mL
    40 mM 0.0318 mL 0.1589 mL 0.3178 mL 0.7944 mL
    DMSO 50 mM 0.0254 mL 0.1271 mL 0.2542 mL 0.6355 mL
    60 mM 0.0212 mL 0.1059 mL 0.2118 mL 0.5296 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Echinacoside Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Echinacoside82854-37-3WntReactive Oxygen SpeciesInhibitorinhibitorinhibit

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