Exatecan Intermediate 2

Cat. No.: HY-W021377 Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Exatecan Intermediate 2 (compound B) is an intermediate of Exatecan (DX-8951, HY-13631). Exatecan is an anticancer agent belonging to the class of camptothecin analogs. Exatecan interferes with the proliferation and division of tumor cells by interacting with DNA, thereby inhibiting tumor growth. Exatecan is primarily used for research of a variety of cancers including ovarian, lung and breast cancers.

For research use only. We do not sell to patients.

Exatecan Intermediate 2 Chemical Structure

CAS No. : 182182-31-6

Size	Price	Stock	Quantity
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500 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
1 g	USD 97	In-stock	Estimated Time of Arrival: December 31
5 g	USD 357	In-stock	Estimated Time of Arrival: December 31
10 g	USD 571	In-stock	Estimated Time of Arrival: December 31
25 g	USD 1143	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Other Forms of Exatecan Intermediate 2:

Exatecan Intermediate 2 hydrochloride Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Camptothecins

Molecular Weight

250.27

Formula

C₁₃H₁₅FN₂O₂

CAS No.

182182-31-6

Appearance

Solid

Color

Off-white to gray

SMILES

CC(NC(CCC1=C2C(N)=CC(F)=C1C)C2=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (399.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9957 mL	19.9784 mL	39.9568 mL
5 mM	0.7991 mL	3.9957 mL	7.9914 mL
10 mM	0.3996 mL	1.9978 mL	3.9957 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (19.98 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (19.98 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (248 KB) RP-HPLC (5555 KB)

Handling Instructions (2659 KB)

References

[1]. Weng W, et al. Antibody-Exatecan Conjugates with a Novel Self-immolative Moiety Overcome Resistance in Colon and Lung Cancer. Cancer Discov. 2023 Apr 3;13(4):950-973. [Content Brief]

[2]. Lv Xianyu, et al. Preparation of camptothecin derivative intermediate. China, CN115197088. 2022-10-18.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9957 mL	19.9784 mL	39.9568 mL	99.8921 mL
	5 mM	0.7991 mL	3.9957 mL	7.9914 mL	19.9784 mL
	10 mM	0.3996 mL	1.9978 mL	3.9957 mL	9.9892 mL
	15 mM	0.2664 mL	1.3319 mL	2.6638 mL	6.6595 mL
	20 mM	0.1998 mL	0.9989 mL	1.9978 mL	4.9946 mL
	25 mM	0.1598 mL	0.7991 mL	1.5983 mL	3.9957 mL
	30 mM	0.1332 mL	0.6659 mL	1.3319 mL	3.3297 mL
	40 mM	0.0999 mL	0.4995 mL	0.9989 mL	2.4973 mL
	50 mM	0.0799 mL	0.3996 mL	0.7991 mL	1.9978 mL
	60 mM	0.0666 mL	0.3330 mL	0.6659 mL	1.6649 mL
	80 mM	0.0499 mL	0.2497 mL	0.4995 mL	1.2487 mL
	100 mM	0.0400 mL	0.1998 mL	0.3996 mL	0.9989 mL