Flocoumafen (Synonyms: WL 108366)

Cat. No.: HY-111186 Purity: 99.17%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Flocoumafen (WL 108366) is an orally active vitamin K epoxide reductase inhibitor and a multi-target ligand, which includes prostaglandin F synthase, serum albumin, glucocorticoid receptor 2, and MMP-9. Flocoumafen is a second-generation anticoagulant rodenticide (ARs) with a half-life of 177.4 hours and has deadly anticoagulant effects.

For research use only. We do not sell to patients.

Flocoumafen Chemical Structure

CAS No. : 90035-08-8

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Other Forms of Flocoumafen:

Flocoumafen (Standard) Get quote

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•Related Proteins:

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MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

MMP-9

Glucocorticoid Receptor 2

In Vitro

Flocoumafen best compound is prostaglandin F synthase and serum albumin (binding affinity ≥ 14.0 kcal/mol), followed by glucocorticoid receptor 2, matrix metalloproteinase-9, nuclear receptor ROR-alpha, and activin receptor type-1, with a binding affinity of ≥ 13.5 kcal/mol^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Flocoumafen (0.02-0.1 mg/kg, once a week for 14 weeks) accumulates residues in the liver of rats and cannot be completely metabolized, showing anticoagulant toxicity at high doses^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Fischer rats^[3]
Dosage:	0.02 and 0.1 mg/kg; once a week; for 14 weeks
Administration:	Oral
Result:	Caused noticeable cell accumulation in the liver, with residuals increasing as the dose rises at low doses, while at high doses, it stabilized after a while. The lethal anticoagulant effected only occurs when the binding sites were saturated. Showed about 30% of the cumulative dosage disappeared from the feces within 3 days after each administration at low doses,for high doses, this value ranges from 18% after the first dose to 59% after the tenth dose, and anticoagulant toxicity appeared after six weeks.

Molecular Weight

542.54

Formula

C₃₃H₂₅F₃O₄

CAS No.

90035-08-8

Appearance

Solid

Color

White to off-white

SMILES

O=C1C(C2CC(C3=CC=C(OCC4=CC=C(C(F)(F)F)C=C4)C=C3)CC5=C2C=CC=C5)=C(O)C6=CC=CC=C6O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (184.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8432 mL	9.2159 mL	18.4318 mL
5 mM	0.3686 mL	1.8432 mL	3.6864 mL
10 mM	0.1843 mL	0.9216 mL	1.8432 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.17%

Select Batch:

Data Sheet (273 KB) SDS (788 KB)

COA (247 KB) HNMR (296 KB) RP-HPLC (253 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Nadia Coronado-Posada, et al. In Silico Analysis to Identify Molecular Targets for Chemicals of Concern: The Case Study of Flocoumafen, an Anticoagulant Pesticide. Environ Toxicol Chem. 2021 Jul;40(7):2034-2043. [Content Brief]

[2]. Sébastien Lefebvre, et al. Poisoning by Anticoagulant Rodenticides in Humans and Animals: Causes and Consequences.

[3]. Huckle KR, et al. Elimination and accumulation of the rodenticide flocoumafen in rats following repeated oral administration. Xenobiotica. 1988 Dec;18(12):1465-79. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8432 mL	9.2159 mL	18.4318 mL	46.0796 mL
	5 mM	0.3686 mL	1.8432 mL	3.6864 mL	9.2159 mL
	10 mM	0.1843 mL	0.9216 mL	1.8432 mL	4.6080 mL
	15 mM	0.1229 mL	0.6144 mL	1.2288 mL	3.0720 mL
	20 mM	0.0922 mL	0.4608 mL	0.9216 mL	2.3040 mL
	25 mM	0.0737 mL	0.3686 mL	0.7373 mL	1.8432 mL
	30 mM	0.0614 mL	0.3072 mL	0.6144 mL	1.5360 mL
	40 mM	0.0461 mL	0.2304 mL	0.4608 mL	1.1520 mL
	50 mM	0.0369 mL	0.1843 mL	0.3686 mL	0.9216 mL
	60 mM	0.0307 mL	0.1536 mL	0.3072 mL	0.7680 mL
	80 mM	0.0230 mL	0.1152 mL	0.2304 mL	0.5760 mL
	100 mM	0.0184 mL	0.0922 mL	0.1843 mL	0.4608 mL

Flocoumafen Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flocoumafen90035-08-8WL 108366WL108366WL-108366Glucocorticoid ReceptorMMPMatrix metalloproteinasesSecond-generation anticoagulant rodenticideVitamin K antagonistVKAInhibitorinhibitorinhibit

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