2. Antibody-drug Conjugate/ADC Related
  3. ADC Cytotoxin
  4. Hemiasterlin

Hemiasterlin  (Synonyms: (-)-Hemiasterlin; Milnamide B)

Cat. No.: HY-117371 Purity: 99.77%
COA Handling Instructions

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Hemiasterlin ((-)-Hemiasterlin) is an antimitotic marine natural product with potent anticancer effcts. Hemiasterlin can be used as a cytotoxic payload (ADC Cytotoxin) in antibody-drug conjugates (ADCs).

For research use only. We do not sell to patients.

Hemiasterlin Chemical Structure

Hemiasterlin Chemical Structure

CAS No. : 157207-90-4

Size Price Stock Quantity
1 mg USD 300 In-stock
5 mg USD 765 In-stock
10 mg USD 1230 In-stock
25 mg USD 2460 In-stock
50 mg USD 3950 In-stock
100 mg USD 6320 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Hemiasterlin Related Antibodies

Top Publications Citing Use of Products

View All ADC Cytotoxin Isoform Specific Products:

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Hemiasterlin ((-)-Hemiasterlin) is an antimitotic marine natural product with potent anticancer effcts. Hemiasterlin can be used as a cytotoxic payload (ADC Cytotoxin) in antibody-drug conjugates (ADCs)[1].

IC50 & Target[1]

Pyrrolobenzodiazepines

 

In Vitro

Hemiasterlin is an antimitotic agent by disrupting microtubule dynamics causing mitotic arrest and cell death with low- to sub-nanomolar potencies against several cancer cell lines[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hemiasterlin Related Antibodies
Molecular Weight

526.71

Formula

C30H46N4O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC(/C(C)=C/[C@H](C(C)C)N(C)C([C@H](C(C)(C)C)NC([C@@H](NC)C(C)(C1=CN(C)C2=CC=CC=C12)C)=O)=O)=O
Structure Classification
Initial Source

marine sponges
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (189.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8986 mL 9.4929 mL 18.9858 mL
5 mM 0.3797 mL 1.8986 mL 3.7972 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.77%

COA (248 KB) HNMR (396 KB) LCMS (131 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8986 mL 9.4929 mL 18.9858 mL 47.4644 mL
5 mM 0.3797 mL 1.8986 mL 3.7972 mL 9.4929 mL
10 mM 0.1899 mL 0.9493 mL 1.8986 mL 4.7464 mL
15 mM 0.1266 mL 0.6329 mL 1.2657 mL 3.1643 mL
20 mM 0.0949 mL 0.4746 mL 0.9493 mL 2.3732 mL
25 mM 0.0759 mL 0.3797 mL 0.7594 mL 1.8986 mL
30 mM 0.0633 mL 0.3164 mL 0.6329 mL 1.5821 mL
40 mM 0.0475 mL 0.2373 mL 0.4746 mL 1.1866 mL
50 mM 0.0380 mL 0.1899 mL 0.3797 mL 0.9493 mL
60 mM 0.0316 mL 0.1582 mL 0.3164 mL 0.7911 mL
80 mM 0.0237 mL 0.1187 mL 0.2373 mL 0.5933 mL
100 mM 0.0190 mL 0.0949 mL 0.1899 mL 0.4746 mL
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Hemiasterlin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hemiasterlin157207-90-4(-)-Hemiasterlin Milnamide BADC CytotoxinADC Payloadantimitoticanticancer effctsADCInhibitorinhibitorinhibit

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