Imipramine

Name: Imipramine
Brand: MedChemExpress
SKU: HY-B1490A
Rating: 5.0 (5 reviews)

Cat. No.: HY-B1490A Purity: 98.78%: FAQs
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Imipramine is an orally active tertiary amine tricyclic antidepressant. Imipramine is a Fascin1 inhibitor with antitumor activities. Imipramine also inhibits serotonin transporter with an IC₅₀ value of 32 nM. Imipramine stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine shows neuroprotective and immunomodulatory effects.

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Imipramine Chemical Structure

CAS No. : 50-49-7

Size	Price	Stock	Quantity
5 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 105	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 325	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
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Based on 5 publication(s) in Google Scholar

Other Forms of Imipramine:

5 Publications Citing Use of MCE Imipramine

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

Fascin1, Serotonin Transporter, Autophagy, Apoptosis^[1]^[2]^[3]^[5]
IC₅₀: 32 nM (human placental serotonin transporter)^[5]

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	5.1 nM Compound: Imipramine	Inhibition of human SERT expressed in CHO cell membranes assessed as reduction in [3H]serotonin uptake preincubated for 10 mins followed by [3H]serotonin addition measured after 20 mins by liquid scintillation counting method Inhibition of human SERT expressed in CHO cell membranes assessed as reduction in [3H]serotonin uptake preincubated for 10 mins followed by [3H]serotonin addition measured after 20 mins by liquid scintillation counting method	[PMID: 27865645]
CHO	IC₅₀	8.8 x 10^-10 M Compound: Imipramine	Displacement of [3H]imipramine from human recombinant 5HT transporter expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [3H]imipramine from human recombinant 5HT transporter expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
DG-75	IC₅₀	> 50 μM Compound: Imipramine	Reduction in cell viability of human DG75 cells after 24 hrs by Alamar blue viability assay Reduction in cell viability of human DG75 cells after 24 hrs by Alamar blue viability assay	[PMID: 23385211]
HEK293	IC₅₀	17.1 μM Compound: Imipramine	Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy	[PMID: 18788725]
HEK293	IC₅₀	2.6 nM Compound: 1	Inhibition of human SERT W103A mutant expressed in HEK293 MSR cells assessed as [3H]-5-HT uptake preincubated for 30 mins followed addition of [3H]-5HT and compound for 10 mins by micro-scintillation counter Inhibition of human SERT W103A mutant expressed in HEK293 MSR cells assessed as [3H]-5-HT uptake preincubated for 30 mins followed addition of [3H]-5HT and compound for 10 mins by micro-scintillation counter	[PMID: 27160055]
HEK293	IC₅₀	7.95 μM Compound: Imipramine	Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of ASP+ after 2 mins by fluorescence assay Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of ASP+ after 2 mins by fluorescence assay	[PMID: 28230985]
HeLa	IC₅₀	40.74 μM Compound: Imipramine	Reduction in cell viability of human HeLa cells after 24 hrs by Alamar blue viability assay Reduction in cell viability of human HeLa cells after 24 hrs by Alamar blue viability assay	[PMID: 23385211]
Ventricular myocyte	IC₅₀	4.05 μM Compound: Imipramine	Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes	[PMID: 22761000]
Ventricular myocyte	IC₅₀	8.3 μM Compound: Imipramine	Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes	[PMID: 22761000]

In Vitro

Imipramine (0.5-300 μM, 3 days) inhibits HCT-116 cell viability^[1].
Imipramine (20 μM) inhibits cell migration (7 h) and invasion (48 h)^[1].
Imipramine (50 μM, 0-240 min) inhibites the PI3K/Akt/mTOR signaling pathway in U-87MG glioma cells^[2].
Imipramine (60 μM, 24 h) stimulates U-87MG glioma cells autophagy^[2].
Imipramine (80 μM, 24 h) induces HL-60 cell apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	DLD-1, HCT-116, and SW-480
Concentration:	0.5-300 μM
Incubation Time:	3 days
Result:	Inhibited cell viability and HCT-116 was more sensitive than DLD-1 and SW-480.

Cell Migration Assay ^[1]

Cell Line:	DLD-1, HCT-116, and SW-480
Concentration:	20 μM
Incubation Time:	7 h
Result:	Produced a remarkable inhibition of migration in all assayed cell lines.

Cell Invasion Assay^[1]

Cell Line:	HCT-116
Concentration:	20 μM
Incubation Time:	48 h
Result:	Inhibited cell invasion through Matrigel.

Western Blot Analysis^[2]

Cell Line:	U-87MG
Concentration:	50 μM
Incubation Time:	0, 15, 30, 60, 120 and 240 min
Result:	Markedly inhibited the phosphorylation of both Akt (Ser473) and mTOR (Ser2481) in a time-dependent manner. Also dephosphorylated p70 S6K, a downstream target of mTOR.

Cell Autophagy Assay^[2]

Cell Line:	U-87MG
Concentration:	60 μM
Incubation Time:	24 h
Result:	Stimulated the induction of autophagy through the redistribution of LC3 in U-87MG glioma cells.

Apoptosis Analysis^[3]

Cell Line:	HL-60
Concentration:	80 μM
Incubation Time:	24 h
Result:	Induced cell apoptosis.

In Vivo

Imipramine (20 mg/kg, i.p. or 15 mg/kg, p.o.; daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice (6–8 weeks old) subjected to RSD (repeated social defeat) and HCC (home cage control)^[4]
Dosage:	20 mg/kg or 15 mg/kg
Administration:	Intraperitoneal injection or oral administration, daily for 24 days
Result:	Reversed RSD-induced social avoidance behavior, significantly increasing the interaction time, significantly decreased stress-induced mRNA levels for IL-6 in brain microglia.

Clinical Trial

Molecular Weight

280.41

Formula

C₁₉H₂₄N₂

CAS No.

50-49-7

Appearance

Liquid (Density: 1.041±0.06 g/cm³)

Color

Colorless to light yellow

SMILES

CN(C)CCCN1C2=CC=CC=C2CCC3=CC=CC=C31

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (891.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5662 mL	17.8310 mL	35.6621 mL
5 mM	0.7132 mL	3.5662 mL	7.1324 mL
10 mM	0.3566 mL	1.7831 mL	3.5662 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.78%

Select Batch:

Data Sheet (285 KB) SDS (584 KB)

COA (255 KB) HNMR (255 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Alburquerque-González B, et al. New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells. Exp Mol Med. 2020 Feb;52(2):281-292. [Content Brief]

[2]. Jeon SH, et al. The tricyclic antidepressant imipramine induces autophagic cell death in U-87MG glioma cells. Biochem Biophys Res Commun. 2011 Sep 23;413(2):311-7. [Content Brief]

[3]. Xia Z, et al. The antidepressants imipramine, clomipramine, and citalopram induce apoptosis in human acute myeloid leukemia HL-60 cells via caspase-3 activation. J Biochem Mol Toxicol. 1999;13(6):338-47. [Content Brief]

[4]. Ramirez K, et al. Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Brain Behav Immun. 2015 May;46:212-20. [Content Brief]

[5]. Balkovetz DF, et al. Evidence for an imipramine-sensitive serotonin transporter in human placental brush-border membranes. J Biol Chem. 1989 Feb 5;264(4):2195-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5662 mL	17.8310 mL	35.6621 mL	89.1552 mL
	5 mM	0.7132 mL	3.5662 mL	7.1324 mL	17.8310 mL
	10 mM	0.3566 mL	1.7831 mL	3.5662 mL	8.9155 mL
	15 mM	0.2377 mL	1.1887 mL	2.3775 mL	5.9437 mL
	20 mM	0.1783 mL	0.8916 mL	1.7831 mL	4.4578 mL
	25 mM	0.1426 mL	0.7132 mL	1.4265 mL	3.5662 mL
	30 mM	0.1189 mL	0.5944 mL	1.1887 mL	2.9718 mL
	40 mM	0.0892 mL	0.4458 mL	0.8916 mL	2.2289 mL
	50 mM	0.0713 mL	0.3566 mL	0.7132 mL	1.7831 mL
	60 mM	0.0594 mL	0.2972 mL	0.5944 mL	1.4859 mL
	80 mM	0.0446 mL	0.2229 mL	0.4458 mL	1.1144 mL
	100 mM	0.0357 mL	0.1783 mL	0.3566 mL	0.8916 mL

Imipramine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Imipramine50-49-7Serotonin TransporterAutophagyApoptosis5-HTTSERTSLC6A4colorectal cancer cellsevere chronic depressionimmunomodulatoryneuroprotectiveInhibitorinhibitorinhibit

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Imipramine

Customer Review

Other Forms of Imipramine:

Imipramine Related Antibodies

5 Publications Citing Use of MCE Imipramine

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•Related Screening Libraries:

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•Related Proteins:

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Purity & Documentation

References

Customer Review

Imipramine Related Antibodies

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Complete Stock Solution Preparation Table

Imipramine Related Classifications

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