2. Apoptosis
  3. MDM-2/p53
  4. MB710

MB710, an aminobenzothiazole derivative, is a stabilizer of oncogenic p53 mutation Y220C. MB710 binds tightly to the Y220C pocket and stabilizes p53-Y220C, with a Kd of 4.1 μM. MB710 shows anticancer activity in p53-Y220C cell lines.

For research use only. We do not sell to patients.

MB710 Chemical Structure

MB710 Chemical Structure

CAS No. : 2230044-57-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 660 In-stock
Solution
10 mM * 1 mL in DMSO USD 660 In-stock
Solid
5 mg USD 600 In-stock
10 mg USD 1050 In-stock
25 mg USD 2250 In-stock
50 mg USD 3600 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

MB710 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MB710, an aminobenzothiazole derivative, is a stabilizer of oncogenic p53 mutation Y220C. MB710 binds tightly to the Y220C pocket and stabilizes p53-Y220C, with a Kd of 4.1 μM. MB710 shows anticancer activity in p53-Y220C cell lines[1].

IC50 & Target

Kd: 4 μM (p53-Y220C)
Cellular Effect
Cell Line Type Value Description References
NUGC-3 IC50
90 μM
Compound: MB710; 70
Antiproliferative activity against human NUGC3 cells harboring p53 Y220C mutant after 72 hrs by CellTiter-Glo assay
Antiproliferative activity against human NUGC3 cells harboring p53 Y220C mutant after 72 hrs by CellTiter-Glo assay
[PMID: 29702446]
NUGC-4 IC50
120 μM
Compound: MB710; 70
Antiproliferative activity against human NUGC4 cells harboring wild-type p53 after 72 hrs by CellTiter-Glo assay
Antiproliferative activity against human NUGC4 cells harboring wild-type p53 after 72 hrs by CellTiter-Glo assay
[PMID: 29702446]
SW1088 IC50
> 120 μM
Compound: MB710; 70
Antiproliferative activity against human SW1088 cells harboring p53 Y220C mutant after 72 hrs by CellTiter-Glo assay
Antiproliferative activity against human SW1088 cells harboring p53 Y220C mutant after 72 hrs by CellTiter-Glo assay
[PMID: 29702446]
WI-38 IC50
> 120 μM
Compound: MB710; 70
Antiproliferative activity against human WI38 cells after 72 hrs by CellTiter-Glo assay
Antiproliferative activity against human WI38 cells after 72 hrs by CellTiter-Glo assay
[PMID: 29702446]
In Vitro

MB710 (0-200 μM; 72 hours) shows relatively low toxicity against all cell lines tested at concentrations up to 60 μM, while showing initial selective viability reduction at higher concentrations[1].
MB710 (72 hours) treats cancer cell lines NUGC3, NUGC4, WI38 and SW1088, with IC50s of 90, 120, >120, >120 μM, respectively[1].
MB710 (0-120 μM; 72 hours; HUH-7 cells) shows stronger cytotoxic effects in presence of p53-Y220C[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MB710 Related Antibodies

Cell Viability Assay[1]

Cell Line: NUGC3 (mutant p53 Y220C), HUH-7 (mutant p53 Y220C), NUGC4 (p53 WT), HUH-6 cells (p53 WT)
Concentration: 0-200 μM
Incubation Time: 72 hours
Result: Showed relatively low toxicity against all cell lines tested at concentrations up to 60 μM. NUGC3 was the most sensitive cell line.
Molecular Weight

457.29

Formula

C16H16IN3O3S
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C(C1=C2N=C(N(CC)CC)SC2=C(N3C=CC=C3)C(I)=C1O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (36.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1868 mL 10.9340 mL 21.8680 mL
5 mM 0.4374 mL 2.1868 mL 4.3736 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1.25 mg/mL (2.73 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 1.25 mg/mL (2.73 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1868 mL 10.9340 mL 21.8680 mL 54.6699 mL
5 mM 0.4374 mL 2.1868 mL 4.3736 mL 10.9340 mL
10 mM 0.2187 mL 1.0934 mL 2.1868 mL 5.4670 mL
15 mM 0.1458 mL 0.7289 mL 1.4579 mL 3.6447 mL
20 mM 0.1093 mL 0.5467 mL 1.0934 mL 2.7335 mL
25 mM 0.0875 mL 0.4374 mL 0.8747 mL 2.1868 mL
30 mM 0.0729 mL 0.3645 mL 0.7289 mL 1.8223 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

MB710 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MB7102230044-57-0MB 710MB-710MDM-2/p53oncogenicaminobenzothiazoleNUGC4HUH-6NUGC3HUH-7cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
MB710
Cat. No.:
HY-120373
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.