2. Metabolic Enzyme/Protease
  3. E1/E2/E3 Enzyme
  4. ML-792

ML-792 is a potent and selective inhibitor of SAE/SUMO1 and SAE/SUMO2 in enzymatic assays (IC50 values of 3 and 11 nM, respectively) compared with NAE/NEDD8 and UAE/ubiquitin (IC50> values of 32 μM and >100 μM, respectively).

For research use only. We do not sell to patients.

ML-792 Chemical Structure

ML-792 Chemical Structure

CAS No. : 1644342-14-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 303 In-stock
Solution
10 mM * 1 mL in DMSO USD 303 In-stock
Solid
5 mg USD 250 In-stock
10 mg USD 350 In-stock
25 mg USD 550 In-stock
50 mg USD 750 In-stock
100 mg USD 1250 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 24 publication(s) in Google Scholar

ML-792 Related Antibodies

Top Publications Citing Use of Products

    ML-792 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2020 Jul 2;79(1):54-67.e7.  [Abstract]

    Treatment of cells with the SUMO inhibitor ML-792 for 4 or 24 h causes a dramatic recruitment of FUSP525L in G3BP1-positive SGs. By contrast, wild-type FUS does not accumulate in these structures upon inhibition of SUMOylation.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ML-792 is a potent and selective inhibitor of SAE/SUMO1 and SAE/SUMO2 in enzymatic assays (IC50 values of 3 and 11 nM, respectively) compared with NAE/NEDD8 and UAE/ubiquitin (IC50> values of 32 μM and >100 μM, respectively)[1].

    IC50 & Target

    IC50: 3 nM (SAE/SUMO1), 11 nM (SAE/SUMO2)[1]
    In Vitro

    ML-792 (0.0007-5 μM; 4 hours) inhibits SAE and SUMO-pathway activities in HCT116 cells[1].
    ML-792 (0.001-10 μM; 72 hours ) inhibits cell proliferation and decreases cancer cell viability in MDA-MB-468, MDA-MB-231, HCT116, Colo-205, and A375[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ML-792 Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Human breast cancer cells MDA-MB-468 and MDA-MB-231; human colon carcinoma cells HCT116 and Colo-205; human melanoma cell line A375
    Concentration: 0.001, 0.01, 0.1, 1, 10 μM
    Incubation Time: 72 hours
    Result: Demonstrated a dose-dependent viability effect with EC50 values of 0.06 μM in MDA-MB-468 cells to 0.45 μM in A375 cells.

    Western Blot Analysis[1]

    Cell Line: HCT116 cells
    Concentration: 0, 0.0007, 0.002, 0.007, 0.02, 0.06, 0.19, 0.56, 1.7, 5 μM
    Incubation Time: 4 hours
    Result: Revealed a dose-dependent decrease in the SAE and UBC9 thioester levels.
    Molecular Weight

    551.41

    Formula

    C21H23BrN6O5S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=S(OC[C@@H]1[C@@H](O)C[C@H](NC2=NC=NC=C2C(C3=NN(CC4=CC=CC(Br)=C4)C=C3)=O)C1)(N)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (181.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8135 mL 9.0677 mL 18.1353 mL
    5 mM 0.3627 mL 1.8135 mL 3.6271 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (3.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.90%

    COA (248 KB) LCMS (296 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8135 mL 9.0677 mL 18.1353 mL 45.3383 mL
    5 mM 0.3627 mL 1.8135 mL 3.6271 mL 9.0677 mL
    10 mM 0.1814 mL 0.9068 mL 1.8135 mL 4.5338 mL
    15 mM 0.1209 mL 0.6045 mL 1.2090 mL 3.0226 mL
    20 mM 0.0907 mL 0.4534 mL 0.9068 mL 2.2669 mL
    25 mM 0.0725 mL 0.3627 mL 0.7254 mL 1.8135 mL
    30 mM 0.0605 mL 0.3023 mL 0.6045 mL 1.5113 mL
    40 mM 0.0453 mL 0.2267 mL 0.4534 mL 1.1335 mL
    50 mM 0.0363 mL 0.1814 mL 0.3627 mL 0.9068 mL
    60 mM 0.0302 mL 0.1511 mL 0.3023 mL 0.7556 mL
    80 mM 0.0227 mL 0.1133 mL 0.2267 mL 0.5667 mL
    100 mM 0.0181 mL 0.0907 mL 0.1814 mL 0.4534 mL
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    ML-792 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ML-7921644342-14-2ML792ML 792E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

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