ML-792

Name: ML-792
Brand: MedChemExpress
SKU: HY-108702
Rating: 5.0 (24 reviews)

Cat. No.: HY-108702 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ML-792 is a potent and selective inhibitor of SAE/SUMO1 and SAE/SUMO2 in enzymatic assays (IC₅₀ values of 3 and 11 nM, respectively) compared with NAE/NEDD8 and UAE/ubiquitin (IC₅₀> values of 32 μM and >100 μM, respectively).

For research use only. We do not sell to patients.

ML-792 Chemical Structure

CAS No. : 1644342-14-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 303	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 303	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 24 publication(s) in Google Scholar

23 Publications Citing Use of MCE ML-792

: ML-792 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2020 Jul 2;79(1):54-67.e7. [Abstract]; Treatment of cells with the SUMO inhibitor ML-792 for 4 or 24 h causes a dramatic recruitment of FUSP525L in G3BP1-positive SGs. By contrast, wild-type FUS does not accumulate in these structures upon inhibition of SUMOylation.

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Description

IC₅₀ & Target

IC50: 3 nM (SAE/SUMO1), 11 nM (SAE/SUMO2)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	EC₅₀	0.09 μM Compound: ML-792	Cytotoxicity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 33631934]
OCI-Ly10	EC₅₀	0.055 μM Compound: ML-792	Cytotoxicity against human OCI-LY10 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human OCI-LY10 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 33631934]

In Vitro

ML-792 (0.0007-5 μM; 4 hours) inhibits SAE and SUMO-pathway activities in HCT116 cells^[1].
ML-792 (0.001-10 μM; 72 hours ) inhibits cell proliferation and decreases cancer cell viability in MDA-MB-468, MDA-MB-231, HCT116, Colo-205, and A375^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Human breast cancer cells MDA-MB-468 and MDA-MB-231; human colon carcinoma cells HCT116 and Colo-205; human melanoma cell line A375
Concentration:	0.001, 0.01, 0.1, 1, 10 μM
Incubation Time:	72 hours
Result:	Demonstrated a dose-dependent viability effect with EC₅₀ values of 0.06 μM in MDA-MB-468 cells to 0.45 μM in A375 cells.

Western Blot Analysis^[1]

Cell Line:	HCT116 cells
Concentration:	0, 0.0007, 0.002, 0.007, 0.02, 0.06, 0.19, 0.56, 1.7, 5 μM
Incubation Time:	4 hours
Result:	Revealed a dose-dependent decrease in the SAE and UBC9 thioester levels.

Molecular Weight

551.41

Formula

C₂₁H₂₃BrN₆O₅S

CAS No.

1644342-14-2

Appearance

Solid

Color

White to off-white

SMILES

O=S(OC[C@@H]1[C@@H](O)C[C@H](NC2=NC=NC=C2C(C3=NN(CC4=CC=CC(Br)=C4)C=C3)=O)C1)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (181.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8135 mL	9.0677 mL	18.1353 mL
5 mM	0.3627 mL	1.8135 mL	3.6271 mL
10 mM	0.1814 mL	0.9068 mL	1.8135 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) LCMS (296 KB)

Handling Instructions (2659 KB)

References

[1]. He X, et al. Probing the roles of SUMOylation in cancer cell biology by using a selective SAE inhibitor. Nat Chem Biol. 2017 Nov;13(11):1164-1171. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8135 mL	9.0677 mL	18.1353 mL	45.3383 mL
	5 mM	0.3627 mL	1.8135 mL	3.6271 mL	9.0677 mL
	10 mM	0.1814 mL	0.9068 mL	1.8135 mL	4.5338 mL
	15 mM	0.1209 mL	0.6045 mL	1.2090 mL	3.0226 mL
	20 mM	0.0907 mL	0.4534 mL	0.9068 mL	2.2669 mL
	25 mM	0.0725 mL	0.3627 mL	0.7254 mL	1.8135 mL
	30 mM	0.0605 mL	0.3023 mL	0.6045 mL	1.5113 mL
	40 mM	0.0453 mL	0.2267 mL	0.4534 mL	1.1335 mL
	50 mM	0.0363 mL	0.1814 mL	0.3627 mL	0.9068 mL
	60 mM	0.0302 mL	0.1511 mL	0.3023 mL	0.7556 mL
	80 mM	0.0227 mL	0.1133 mL	0.2267 mL	0.5667 mL
	100 mM	0.0181 mL	0.0907 mL	0.1814 mL	0.4534 mL

ML-792 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML-7921644342-14-2ML792ML 792E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

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ML-792

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ML-792 Related Antibodies

23 Publications Citing Use of MCE ML-792

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Complete Stock Solution Preparation Table

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